FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD_S3_R2_B10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R2_B10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTTAGCATGTTGAACATGCCCATCATCATCCCAGGACTCAGTGATGC23.7037037037037033No Hit
GATCCAGGTTCCAGGTTGTCCCTAAGTGCCTGAACAATCAGAACCAATTT11.8518518518518516No Hit
GAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATACGTCCATA11.8518518518518516No Hit
CATCGACGAACGGGGTGAATCAATAATTGTAGAATCTGGTGCCCCGAATG11.8518518518518516No Hit
GTATGGATAGCAAATAGTAGCATTGACACAACTACTTCAGTGCATGTGTG11.8518518518518516No Hit
CTCAGAGAACTCTTCTTTCTTGATCCGACCAGACTCGAAGTCGCCCCTGG11.8518518518518516No Hit
CCATTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA11.8518518518518516No Hit
TATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCTGG11.8518518518518516No Hit
GTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGCCGCATTTCTTCAATT11.8518518518518516No Hit
CTATAAGACAAGAAATGGCCAGTAGGAGACAATGGGATACCTAACCACAG11.8518518518518516No Hit
GGAACAGGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGA11.8518518518518516No Hit
ATGGAAGACTTTGTGCGACAATGCTACAATCCAATGATCCTCGAGCTTGC11.8518518518518516No Hit
AGCAGGAACTGATCCAAAATGGAAGACATTCTGCGACAATGCTTCAATCA11.8518518518518516No Hit
AATATATTCAAAATGGAGAGAATAAAAGAACTGAGAGATCTAATGACGCA11.8518518518518516No Hit
GGAGAAGAGCAACAGCTATTCTCAGAAAGGCAACCACGAGATTGATCCAG11.8518518518518516No Hit
CTATTGTTCTTTGCGTGACCATCTTCTTGGTCATGTTGTCTCTTACTCTC11.8518518518518516No Hit
TCGTGGAACAGATCTTCAAGATCACAGACAACTCATCTGTCTTTATCAGT11.8518518518518516No Hit
GTGTAGTTTCAACGGCATCATATTCCCTGCTTTTGGCTGGACCATGTGCT11.8518518518518516No Hit
ATATAATGGTATTCCCTCCCCCTGGTGGCTCGGGTCGAGAATACTTAATT11.8518518518518516No Hit
ATCATATTCCATGCTTTTGCCTGGACCATGGGCTGGCATTACCACAGCAT11.8518518518518516No Hit
GATGCAGAGAGAGGCAAGTTAAAAAGAAGGGCTATCGCAACACCTGGGAT11.8518518518518516No Hit
GGCTGGATCGAGTGAACAGGCAGCGGAGGCCATGGAGGTTGCTAATCAGA11.8518518518518516No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA11.8518518518518516No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT11.8518518518518516No Hit
ACATAGTACTGAAAGAGAACTTCAGTGTAAACTTTAACCGATTAGAGACC11.8518518518518516No Hit
CAGGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAG11.8518518518518516No Hit
GAGAAAAGCTGTTGCACAACTCAAAACCATACAGAATTAATCATACACTT11.8518518518518516No Hit
GAATAGAACGATACCTCTTGGGAATCCAGGAAAGTGTAGTTGCAACGGCA11.8518518518518516No Hit
CTCGTGACTCGGGTCTTCAATACTTAATTTCAGAGCATCCATCAGCAGGA11.8518518518518516No Hit
CATCTGATCATCCTCAAGAATTCCCCTTAGGCGTGTGTTGAGAATAGAAC11.8518518518518516No Hit
CACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACTGGTGC11.8518518518518516No Hit
ATTCGAGACTAATTGATGGCCATCCGAATTCATTTGGTCGCTTTCTGGCT11.8518518518518516No Hit
CCCAGGGATCATTAATCAGGCACTCCTAGATTGCTTCATATAGCCCCACC11.8518518518518516No Hit
CAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGA11.8518518518518516No Hit
GCCTTCTTCAGTCTCTTCAGCAGATTGAGAGCATGATTGAGGCCGAGTCT11.8518518518518516No Hit
GGCCAAGTTAAATTCATTATATTTGCCGTCTGAGTACTTCAATGGTGGAA11.8518518518518516No Hit
CTCTTTGCGTGCGATTGGACCAGGCGATCATGGAAAAGAACATAGTACTG11.8518518518518516No Hit
AATGTGCAATATCCTCAAAGGAAAATTTCAAACAGCTGCCCAGAGGGCAA11.8518518518518516No Hit
GTGTACATAAATACGGCCTTGCTCAATGCATCCTGTGCAGCCATGGATGA11.8518518518518516No Hit
GGCAGTGGCTGTGTTAAAGTACAACGGCATAATAACAGACACTATCAACA11.8518518518518516No Hit
GAGATACCCAATTACAGCAGACAAGAGAATAATGATTCCAGAGAGGAATG11.8518518518518516No Hit
CCTTATTTTAACTTGATTTCTGAAGTGGACAGGGCCGAAGGTACAATGTT11.8518518518518516No Hit
GCTTGTTTTACGGACCAATTCTCTTTCTAGCATGGCCGCCACCATCAAGG11.8518518518518516No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA11.8518518518518516No Hit
CTAGCAAGCTGGCTCCAAAATGAATTCAATAAGGCATGTGAAATGACTGA11.8518518518518516No Hit
GTCCCCTTCTCAGTTCCTTGCGTTTCACTGACTTCTTCGGGAGACACTAG11.8518518518518516No Hit
TCTCAACACAAGCCAAAGGGGAATTCTTGAGGATGAACAGATGTACCAGA11.8518518518518516No Hit
GATTTAAGAATATCAAGTTTCATAAGAGGAAAGAAAGAGATTCCAAGAGG11.8518518518518516No Hit
GGGAGATTCCGCTGCACTGAGAATGTAGGCTGCACACTGATCTGGCCTGC11.8518518518518516No Hit
GAACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGA11.8518518518518516No Hit
TCATTATGTATTCAGTAGCCCTGCAGTGGGACACTTCTGCTGTAAAATAG11.8518518518518516No Hit
ATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAAAATGCACA11.8518518518518516No Hit
GTCTATATGCGTCTCCACAACTTCAGGGGTTTTCGGCTGAATCTAGAAAA11.8518518518518516No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAAAGA50.0145.04
AGAACTG50.0145.08
AAAAGAA50.0145.05
GAACTGA50.0145.09
ATAAAAG50.0145.03
AAGAACT50.0145.07
GAATAAA50.0145.01
AATAAAA50.0145.02
AAAGAAC50.0145.06
TGGCCCA50.0145.0145
Produced by FastQC (version 0.11.5)