FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R2_B05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R2_B05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAATAGAGATAACAACCCACTTTCAAAGAAAAAGGAGAGTAAGAGACAA15.263157894736842No Hit
CCACAGCAATGTTTACAGAATCAATCTATTTATGAATGACAAAGGGATTC15.263157894736842No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG15.263157894736842No Hit
GCACTATTGAAGCACCGATTTTAGATAATTGAAGGCAGAGACCGACAGGA15.263157894736842No Hit
GAAATCAGATGGTGAATATACTAAAATATACACTCAAAATTTTTATGAAA15.263157894736842No Hit
CACCCATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATG15.263157894736842No Hit
CGCAGAGACTGGAAAGTGTCTTTGCAGGAAAGAACACAGATCTTGCGGCG15.263157894736842No Hit
GAATCAACATGAGCAAAAAGAAGTCCTATATAAATAAGACAGGGAAATTT15.263157894736842No Hit
TGGTTATCCCATTAGGCTCTGTCTTCACCAGATTACTTTCTGGACTACTA15.263157894736842No Hit
CCGGTCTCCTTTATGCACTGCAATGGAAAAAATTCACGAAAATATTGCAG15.263157894736842No Hit
GGCATACTGATTCGGATATCTACGTGAAGATACCTAAGTGGCTCAGCAAT15.263157894736842No Hit
AAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGT15.263157894736842No Hit
GTGTCAGCAGACCCATTAGCATCTCTCTTGGAAATGTGCCACAGAACACA15.263157894736842No Hit
TATTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG15.263157894736842No Hit
CTCCAGCACGGAGAGAGTGGTAGTGAGTATTGACCGATTTTTAAGGGTTA15.263157894736842No Hit
CTTAATACCCTTAATGTACTATTATTTTAATTGTTTTTAGGTTTACCGTG15.263157894736842No Hit
GTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGAGGAAAAGCCAAA15.263157894736842No Hit
CCTGTGCTTATGCGCACTCGCTGACTTTCTCTCTGTCAAACAAATAAAAT15.263157894736842No Hit
TTTCTCATGTGGTTTGACTATGTTAGTCTCGTTCCAGCCAAAGAAGGTCG15.263157894736842No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)