FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R2_B04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R2_B04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAGACTCTGAGGCAGAGGACTCTGTTGTGGGAGGAGGTGGATTGTGGG11.0752688172043012No Hit
TCACTACCACTCTCTCCGTGCTGGAGTATTCATCTACTCCCATTTTGCTG11.0752688172043012No Hit
AGTCTCGGGTCAGTGAGTGAGAACTCCATACTTACAAAGTTCACCACATC11.0752688172043012No Hit
TGTTAGGCTCTTTCCAGCCAAAGAATGTCTTCATGCATTTGATTGCATCA11.0752688172043012No Hit
GGCAGTCACTCCACATTTCCTCCTGCCCCACCTCCAGGGCAACCAGTGAT11.0752688172043012No Hit
CACCGTGCCCAGTGAGCGAGGACTGCAGCGTAGACGCTTTGTCCAAAATG11.0752688172043012No Hit
ACACTCCACACCAAAATGGCTGAAATAAAAAAAAAAATGGACAGCAGCAA11.0752688172043012No Hit
GGCGGGTGGCATAGGAAGGGCCCAACAACATGGACAGGTCAGTCCCCGAA11.0752688172043012No Hit
GAGATGAGGACAGATGGGCTCAGGGAGGAGGCAGCTCCCTCCCTCCTCTG11.0752688172043012No Hit
TATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTCCC11.0752688172043012No Hit
TGCATTGTGTAACTTCTTCCTTCCCTGAGCCTCCATTTCCATGGGTGTAA11.0752688172043012No Hit
CTTGTCAATGGTAAATGGCAACTCAGCACCGTCTGGCCAAGACCAACCCA11.0752688172043012No Hit
GCTGTGAACTGTGTATTCATCTTTTCAATAACAGAATATACTTTGTTAGT11.0752688172043012No Hit
AGATAATTGAAGGAAGAGACCGAATCATGGCCTGGACAGTGGTGAACAGT11.0752688172043012No Hit
GGGTAGAGACCATGGGCTTGTGAGTGGAACCTGTGGTAGTGTGAGTGGCG11.0752688172043012No Hit
CCGAGAGGCTCCAGCCCAAAAGCTGGGAGGTGTGTTCCTTCACCTCCTTT11.0752688172043012No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG11.0752688172043012No Hit
TCCCCGCCCGTCCCGGGGGTTAAGTGCAACCCGTCAATAGAAACACTGCC11.0752688172043012No Hit
CTCAAGAATGGTTCAATTTTGGCGTTCACTTCTTTTGACATTTGGGAAAG11.0752688172043012No Hit
CATAAAAGCCTTGTGACTGCAAGAATTTCCCTCTTCCCAAGCCACAGCCC11.0752688172043012No Hit
CTCTTATAACTTTCTCTTTGTTTCAATACAAAGAAATGCTCAATTAGTTG11.0752688172043012No Hit
GTTGAAGGATATCTGGATTTTTTTTTCCAATTTTTTGCTCTTAGGAATAA11.0752688172043012No Hit
GAATAGAAAGATTCCTCTTGGGAATCCAGGAATGTGTAGTTGCAACGTAA11.0752688172043012No Hit
TCATTAATCAGGCACTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAA11.0752688172043012No Hit
ATGATAACAGCCTCAGCATGGAGGCCTTCCAGCACCGTTCCGTGTCCTGG11.0752688172043012No Hit
AAAGAAGGGAGTGTACATAAATACGGCCTTGCTCAATGCATCCTGTGCAG11.0752688172043012No Hit
GAACTGGGGAATTGAATCCATCGACAATGTGATGGGAATGATCGGAATAC11.0752688172043012No Hit
GCCAATGAGATTGCAACACACAGAGAAAGCTGCACAGTCATGCTAGGTCC11.0752688172043012No Hit
GCACTAAGACCTTCAGGGGCCTAAAAAGAAATTGCCCGCCAAGTGCCTGG11.0752688172043012No Hit
CCTTTCAAATATGGATTAAAGTTACTAACAAATAAGAAGAACAAGGAAAA11.0752688172043012No Hit
ATTCCAGAGAGGAATGAACAAGGACAAACCCTCTGGAGCAAAACAAACGA11.0752688172043012No Hit
AAAAAAAGCTTGTGAATTCAAATGTCCCTGTCTTATTTATATAGGACTTC11.0752688172043012No Hit
GTATAGCATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGT11.0752688172043012No Hit
CAAATGGGAAAAATACTGTGTTCTTGAAATAGGAGACATGCTCTTGAGGA11.0752688172043012No Hit
GGACAGCACAGGGTAAACTGGATTCTGAGGTGGTTTTGCATAAATAAAGG11.0752688172043012No Hit
GAATACCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGG11.0752688172043012No Hit
ACCCTTGGGTGTTTGACAAGTTGTATTGCAATCGTGGACTGGTGTATCTG11.0752688172043012No Hit
GAACAGTGCTGGTGATTGCTCACAGGCTGCAGACGGTGCAGAGTGCGGAC11.0752688172043012No Hit
GGCCTATTTGGGGCCATTGCCGGTTTCATTGAAGGGGGGTGGACAGGGAT11.0752688172043012No Hit
ATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTTTGTCTTGATCCG11.0752688172043012No Hit
TCATTATAGTGGCGGAATTCCAGAAGCAGAATCACGTACTCTGGGAAACC11.0752688172043012No Hit
GCGTGGGGCGGGACCATGGGCTGGCATTACCCCCGCTTTGTGGCCAGCAT11.0752688172043012No Hit
GTCTGCACCATAGCCAAGGCAGAGTTAAGACCACCTTGGAGAGGCAGGCT11.0752688172043012No Hit
CGCCCACCGAACTTCCCCCGCCTTGAAACCTTTCGCGCCGATGTAGATGG11.0752688172043012No Hit
CAGGTGCAAGATCCCAATGATATTTGCTGCAATGACGAGAGGATCACTTG11.0752688172043012No Hit
CTCCAACTCCTGTATGAAGCCATGGGCATTAGCTGCCTGGGCAGCTGCCA11.0752688172043012No Hit
GGCCAAGTCTCCCCCACCCCCCACCTGCGACTGGGACACCGTCACAGGCT11.0752688172043012No Hit
GATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTCC11.0752688172043012No Hit
ACAATGCTTCAATCCAATGATCGTCGAGCTTGCGGAAAAGGCAATGAAAG11.0752688172043012No Hit
GCATGGAGGCCTTCCAGCACCGTTCCGTGTCCTGGTCACAGTTCAATAAG11.0752688172043012No Hit
ATGCATTGAGCAAGGCCGTATTTATGTACACTCCCTTCATTATGTATTCA11.0752688172043012No Hit
GTATACGAAACCTCTAATCTGCATCCCAGGTGTTGCGATAGCCCTTCTTT11.0752688172043012No Hit
GCCCCGAGCCTCTGGGGTCCTGAGGGGCTGCCTGGCTCTTACTTGTGGGC11.0752688172043012No Hit
GGACCAGCCCTGTCTCGAGACCATCAACCGCATCAAGTTGTACAGTAAGT11.0752688172043012No Hit
GCCTGAGCCGAAGGCCGACACTGACCCCACTGAGCCAGCCAGGTGTCCAA11.0752688172043012No Hit
TGGAAAAGAACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGA11.0752688172043012No Hit
GGCTGGCCCTGTCCGGGTCGCCTGGGGAGAGGCAGTAGGCAGGCGTTGTA11.0752688172043012No Hit
GAAATCTCTGTGTCTTGTGAATTAGTCATCATCTTTCTCACAACATTTGC11.0752688172043012No Hit
GTAGAAACAAGGTCCCTTCCAGTTCTCCGTTCCTCTGACTAGCCTAAGGA11.0752688172043012No Hit
CACCATGGCCTCCACCATGCTAGAAATTCCAACCCGTCTCCTATATGAAC11.0752688172043012No Hit
GGCAGCAATAGGGTTGAGGATGAGATCATCTTTCAGTTTTGGTGTGTTCC11.0752688172043012No Hit
GAGTGATCGAATGTAGCAGTCCCGCACTCGCGCGAGCGGCCCTTAGACAA11.0752688172043012No Hit
GGCGGCGAGCATCTGGATTCTAAACGACTTGAGTGTGGGAAGGCCAGACC11.0752688172043012No Hit
AGATTGTGTTCTCTTTGGGTCGCCCTCTGATTAGTTCAACCAAGATGAAA11.0752688172043012No Hit
CGCAAAGAACAATAGGGAAGAAAAAACAAAGACTGAATAAGAGAGGCTAT11.0752688172043012No Hit
CAGCCTCGGGGAGCTGGGGCGGCGGGAGCCTGTATGGTGCCTGGTGTGGG11.0752688172043012No Hit
CTCCAGGTCCAACTGCCCAGATCCTTGGCCCTTATCCCTCCTTTGGTTCC11.0752688172043012No Hit
CAACTGGAAATCTAGTGGTACCGAGATATGCATTCGCAATGGAAAGAAAT11.0752688172043012No Hit
GTCTCCGAAGAAATAAGACCCTTCATTACTCATGTCAAAGGAAGGCACGA11.0752688172043012No Hit
GCTGACACTGCTGCTCTTCTTACTATGTTTCTAGCAGCGATAATCAAACT11.0752688172043012No Hit
GGTTCATGCTCATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGA11.0752688172043012No Hit
CTTTTGCATCTTTTTGGAAATGCCTCAAGAGATGGTGCATGGGGTTCAGT11.0752688172043012No Hit
CCTTCTGCCTCAAGGACTACCCAGAAGACATCGTCTCTTTTGTGATTTCT11.0752688172043012No Hit
GTCTGAATTTGTGTGTCTCCCCTATGGCACCTATATGTGTATCTGTAGTC11.0752688172043012No Hit
CAAGGTAGTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGTCAA11.0752688172043012No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC11.0752688172043012No Hit
GGACAGTGTCAAATGTCCCAAGCACATCCCGCATTTGCTGGAACAGTGTC11.0752688172043012No Hit
GGTCTGGGAAGGCCCTTGGCTTAGATTGCTTTGTTTGATATTAGTGTCCT11.0752688172043012No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTT11.0752688172043012No Hit
CAGTAGCCCTGCAGTGGGACACTTCTGCTGTAAAATAGTTCCTCCTCATG11.0752688172043012No Hit
GGCAGCAATAGGGTTGAGGATTAGCTCATCTTTCAGTTTTGGTGGGTTCA11.0752688172043012No Hit
CCTGCATCCTTTCCAAGAACTGTAAGTCGTTTGGTTGCCTTGTTGTAATT11.0752688172043012No Hit
ACAAAGTCTCTATCAGAATGCAGATGCATATGTTTTTGTGGGGTCATCAA11.0752688172043012No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC11.0752688172043012No Hit
GGATGTGCCTGGAGATGGGGTGGATGAGGAACGGAGAGGCCACGCGGTGG11.0752688172043012No Hit
ATGCACAAACAGACTGTGTTCTAGAGGCTATGGCTTTCCTTGAAGAATCC11.0752688172043012No Hit
CTTTAACACAGCCACTGCCCCATTGTCTGGGCCAGAAATTCCAATTGTTA11.0752688172043012No Hit
CCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGG11.0752688172043012No Hit
TTCTAGCAGCGATAATCAAACTTTGGTCAACATCATCATTTCTCACTTCT11.0752688172043012No Hit
CCATTGACGAGATTACTAACAAAGTAAATTCTGTTATTGAAAAGATGAAT11.0752688172043012No Hit
ATTACTAACAAAGTAAATTCTGTTATTGAAAAGATGAATACACAGTTCAC11.0752688172043012No Hit
GGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGAATTTAAATAAAAA11.0752688172043012No Hit
AATCAGAATGATCAAACGTGGAATCAATGACCGAAATTTCTGGAGGGGTG11.0752688172043012No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGAG50.0145.03
CCAATGA50.0145.02
CGCTCCT50.0145.0145
GATTGCA50.0145.09
GCCAATG50.0145.01
AGATTGC50.0145.08
GAGATTG50.0145.07
AATGAGA50.0145.04
ATGAGAT50.0145.05
TGAGATT50.0145.06