FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R2_A11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R2_A11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2797
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA50.1787629603146228No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG40.14301036825169824No Hit
GCCATGGGGGGTGTTTGAACAGTGGTATGGGGAAGCTGTGATCTGAGCCA40.14301036825169824No Hit
GGGAATGCAGTGTGTGTTGGGCACAAGGCATTTGCGGTGCTGCCTTCCTC30.10725777618877369No Hit
CCTCTACATCCAACACGGGGCTCCAACTCATGACCCCGAGATCAAGAGCT30.10725777618877369No Hit
ACTTAGGACTGGGCTATACATCCAACAGCAGCAGCACTATGGGTCAAAAT30.10725777618877369No Hit
GTGTAGAGGATGGCTTGGAAAGGGAGGGACTGGCCGTACAAAAAGTTCAC30.10725777618877369No Hit
GTCCTGGAATGGTGACCAGAGCTTAAGGCAAACACTTAATCAACTGAGCA30.10725777618877369No Hit
GCGTTGCTCTGAGCCTTTTATGTAACAACAGTCAGTGTGCAAATCAGTGT30.10725777618877369No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACATCTTAACAATA30.10725777618877369No Hit
TGCCATCCCCTCTCCCCCAAAGACCAGAAAATTGCTCCAGGTCCAACTGC30.10725777618877369No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)