FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R2_A07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R2_A07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2483
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG120.4832863471606927No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC110.44301248489730166No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG90.3624647603705195No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA80.3221908981071285No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG70.2819170358437374No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA70.2819170358437374No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT70.2819170358437374No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT60.24164317358034634No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT60.24164317358034634No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG60.24164317358034634No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG60.24164317358034634No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT60.24164317358034634No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT60.24164317358034634No Hit
ACTTAGTGTTGTCCCCAGTGATTGTGAAAGAAATCTCTGTGTCTTGTGAA50.20136931131695532No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG50.20136931131695532No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC50.20136931131695532No Hit
ATATAAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTAT50.20136931131695532No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG50.20136931131695532No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT40.16109544905356424No Hit
GTGTTGGCCAATGCAGTTGCTGCCGGTTGATTTCTGTTTAATGTCCAATC40.16109544905356424No Hit
GGGGAGGGAATACCACTATATGATGCAATCAAATGCATGAAGACATTCTT40.16109544905356424No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG40.16109544905356424No Hit
GACTGAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATG40.16109544905356424No Hit
GTACTATTGTCTCCCGAAGAAGTCAGTGAAACGCAAGGAACTGAGAAGTT40.16109544905356424No Hit
CCCTAAGGCAACCAGAAGCCGGTACAGTGGATTCGTAAGGACACTGTTCC40.16109544905356424No Hit
CTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGA40.16109544905356424No Hit
AAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAG40.16109544905356424No Hit
GAATACCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGG40.16109544905356424No Hit
GTCTGGGCTCCCAGTAGGGGGCAATGAAAAGAAGGCCAAACTGGCAAATG40.16109544905356424No Hit
GGATGTGATTATGGAAGTTGTTTTCCCAAATGAAGTGGGGGCAAGAATAC40.16109544905356424No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG40.16109544905356424No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT40.16109544905356424No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG40.16109544905356424No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC40.16109544905356424No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT40.16109544905356424No Hit
GTATAACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGT40.16109544905356424No Hit
CAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTCTCAATTTTCT40.16109544905356424No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA40.16109544905356424No Hit
ATCTGAGGGGGCGTGGTGTCGTCCTCAAGAATGGTTCAATTTTGGCGTTC30.12082158679017317No Hit
CATCTACATAGGCTCTAAAGTTTTCAAGGCTGGGGAAGTTCGGTGGGAGA30.12082158679017317No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA30.12082158679017317No Hit
GGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGA30.12082158679017317No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA30.12082158679017317No Hit
GTCTTGGGAGTCTCGATACTGAATCTTGGACAAAAGAAATACACCAAGAC30.12082158679017317No Hit
GTCTTATTTATATAGGACTTCTTTTTGCTCATGTTGATTCCCACTAACTT30.12082158679017317No Hit
CCTCAAGAGCATGTCTCCTATTTCAAGAACACAGTATTTTTCCCATTTGT30.12082158679017317No Hit
ATCTAATAAGAGCACTGACATTAAATACGATGACCAAAGATGCAGAGAGA30.12082158679017317No Hit
CTTTTGACATTTGGGAAAGCTTGCCCTCAATGCAGCCGTTCGGCTCGAAT30.12082158679017317No Hit
CTCAAGAATTCCCCTTTGGCTTGTGTTGAGAATAGAACGATTCCTCTTGG30.12082158679017317No Hit
GCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAG30.12082158679017317No Hit
AGTCAGTGAAACGCAAGGAACTGAGAAGTTGACAATAACTTATTCGTCAT30.12082158679017317No Hit
GCACCAAACCATGAGGGAATACAAGCAGGAGTGGACAGATTCTACAGGAC30.12082158679017317No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA30.12082158679017317No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG30.12082158679017317No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT30.12082158679017317No Hit
CATTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTC30.12082158679017317No Hit
GGGTGAATCAATAATTGTAGAATCTGGTGACCCGAATGCACTATTGAAGC30.12082158679017317No Hit
CTCTTGAGGACTGCGATAGGCCAAGTGTCGAGGCCCATGTTCCTATATGT30.12082158679017317No Hit
GTACTTAGCATGTTGAACATGCCCATCATCATCCCAGGACTCAGTGATGC30.12082158679017317No Hit
CTTGTGAATTAGTCATCATCTTTCTCACAACATTTGCCAGTTTGGCCTTC30.12082158679017317No Hit
CTTTAGAGCCTATGTAGATGGATTCGAGCCGAACGGCTGCATTGAGGGCA30.12082158679017317No Hit
GCACCCATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACAT30.12082158679017317No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC30.12082158679017317No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA30.12082158679017317No Hit
GTAAAATTGCTCCCTTGATGGTGGCGTACATGCTAGAAAGAGAATTGGTC30.12082158679017317No Hit
GTTCCAGGTTGTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGAT30.12082158679017317No Hit
CTTTGTAAGTATGGAGTTCTCACTCACTGACCCGAGACTGGAGCCACACA30.12082158679017317No Hit
ATTACATCCTTTAAGAAATCTATTAGCCTTCCTGACTCATTAGCTGTTAG30.12082158679017317No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA30.12082158679017317No Hit
GCGGAAAAGGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAA30.12082158679017317No Hit
ATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCC30.12082158679017317No Hit
GAGTAAGAGACAACATGACCAAGAAGATGGTCACGCAAAGAACAATAGGG30.12082158679017317No Hit
GCACTATTGAAGCACCGATTTGAGATAATTGAAGGAAGAGACCGAATCAT30.12082158679017317No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC30.12082158679017317No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATAA100.0066706426144.979585
Produced by FastQC (version 0.11.5)