FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R2_A05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R2_A05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAATAATTGTGGCCATGGTATTCTCACAGGAGGATTGCATGATCAAGG25.0No Hit
ATTACATCCTTTAAGAAATCTATTAGCCTTCCTGACTCATTAGCTGTTAG12.5No Hit
TACCAGCAGAAATGCTAGCAAGCATTGACCTGAAGTACTTAAATGAATCA12.5No Hit
TACAGATACACATATAGGTGCCATAGGGGAGCCACACAAATTCAGACGAG12.5No Hit
GCCAGCATCCCCACCGGACCGTCTACTGTCGATCCTCCCTACAGAACGGT12.5No Hit
GTATGGATAGCAAATAGAAGCATTGCCACACCTACTTCAGTGCATGTGTG12.5No Hit
CTCTAATTCCTCTTTGTTAATTCAATACAAAAAATCCTTAAACATATCTA12.5No Hit
AGCTAAAGTTTCAACAAAGTATACGAAACCTCTAATCTGCATCCCAGGTG12.5No Hit
GTACTATTGTCTCCCGAAGAAGTCAGTGAAACGCAAGGAACTGAGAAGTT12.5No Hit
CTGTATATTTTCAGAGACTCGAACCGTGTTACCATTCCATTCAAGTCATC12.5No Hit
TTAGATAGCCTCTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTT12.5No Hit
CTAGCAAGCTGGGTCCAAAATGAATTCAATAAGGCATGTGAATTGACTGA12.5No Hit
ACACTAAGTGGAATGAAAATCAAAATCCTCGAATGTTCCTGGCGATGATT12.5No Hit
TCATTTACTCTTAATTTCAGTGTTTGGAGGTTGCCCGTTAGCACTTCTTC12.5No Hit
ATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTCCC12.5No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGGTTTCGTTTCATTACCAA12.5No Hit
CTGGTGCACCCCAGCTCAACCCGATTGATGGACCACTACCTGAGGATAAT12.5No Hit
GAAAGAATGTGCAATATCCTCAAAGGAAAATTTCAAACAGCTGCCCAGAG12.5No Hit
GTTCCCGCAAATGCGGGATGTGCTTGGGACATTTGACACTGTCCAAATAA12.5No Hit
CAGAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGA12.5No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG12.5No Hit
ATAATATACAATTAAAAAAGTTAATTTAGAAATATCACCAAATATATATC12.5No Hit
GGACAAACCCTCTGGAGCAAAACAAACGATGCTGGATCAGACCGAGTGAT12.5No Hit
CGGTTCCTCATACTTAAGGCAGGTTCCGCTGCAGTCTCTTTGATCCGTGG12.5No Hit
CCTCTCGACCGATTAATAACAACTGGCACAATCTCCATGTACCCAATCAG12.5No Hit
GTCATACTCATAAGAATTGTCACAGAAGAAAAAACACACTTCATTAATAA12.5No Hit
CCATTGCATTTGGGTAAATGTAACATTGCTGGCTGGATCCTGGGAAATCC12.5No Hit
AAATTAGAATGATATGACTATATAACCCTTATATAATCTAAATAGGGTTT12.5No Hit
GTTCAGATCGCCCCTAACTGCCTTGATCATGCAATCCTCCTGTGAGAATA12.5No Hit
ACCTAGAGAAAGCCAACAAAATAAAATCTGAGAAGACACACATTCACATC12.5No Hit
CTAACAAGTTTGCTGCAATATGCACACATTTGGAAGTTTGTTTCATGTAT12.5No Hit
GTTCAGTCAACATCACATTTACTTTTACCAGTTGTTTCTACTGGGTGTTC12.5No Hit
GCTAATTTTAGCATGGAGCTACCCAGCTTTGGACTGGCTGGAGTAAATGA12.5No Hit
TATTTAATGTCAGTGCTCTTATTAGATAGCCTCTCTTATTCAGTATTTGT12.5No Hit
TTCCAGCATTACCCCATCAATTACATCTCAGTATAATTTTGCTTCCACTG12.5No Hit
CTCAAATACCAGAAGAAAAGCTAGCAAACATTTACCCGAAGTACTTCAAT12.5No Hit
CCTCTGACTAAGGGAATTTTAGGATTTGTGTTCACGCTCACCGTGCCCAG12.5No Hit
TCGGAATACTGCCCGACATGACCCCAAGCACGGAGATGTCGCTGAGAGGG12.5No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA12.5No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers