FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R2_A04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R2_A04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA21.6129032258064515No Hit
CAGCAAAACTTAACACTGACTTAAGTTGCCTCAAGGGAAGACCGTCAAAG10.8064516129032258No Hit
GCGGCCCGCATGCTCTCAATCTGCTGGAGCGACTGAAGACGGAAGCGCCT10.8064516129032258No Hit
CAACAAACAAGGGTAGATAAACTAACTCAAGGTCGCCAGACTTATGATTG10.8064516129032258No Hit
GTCCACATATATTACCTAGGGAAAGCCAACAAAATAAAATCTGAGAAGAC10.8064516129032258No Hit
ATAAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGG10.8064516129032258No Hit
GTGAATGCCCCATAGCACGAGGACTTCTTTCCCTTTATCATTAATGTAGG10.8064516129032258No Hit
GGGGAGGAGGTGGTGTGGGAGACGAGTTTGAGTGAAGGGCGCCCAGGAAG10.8064516129032258No Hit
GTAATCCACAATATCAGGTGCAAGATCCCAATGATATTTGCTGCAATGAC10.8064516129032258No Hit
GGCCTGGACAGTGGTGAACAGTATATGTAACACAACAGGGGTAGAGAAGC10.8064516129032258No Hit
GTATAAATGCAGATTCCCTGGCCTCCTGAGCCACACGTGAGGGACGGAGT10.8064516129032258No Hit
ATTCTGTACCCCCTGTGGTTTGTCTTTGCGCCCTCATCACAGTGGAGGTT10.8064516129032258No Hit
GGAGGAGATAGGGCGAAGGGAGGGAGAGACTCTGAGGCAGAGTACTCGGT10.8064516129032258No Hit
TTATGGGCTTGTGGCAAGTCCAGAGGTCGCTTCTTTTATTTTAACGAAAT10.8064516129032258No Hit
GAGAAAGCCCCAGGGACTCACATTCCAGAGCTTTCTCCTGACTCACCCTG10.8064516129032258No Hit
GCCTGCCTCTCTGCCTACTTGTGATCTCTGTCAAATAAATTTAAAAAAAA10.8064516129032258No Hit
AGGGGAGACACACAAATTCAGACGAGAAGATCATTTGAGTTAAAGAAGCT10.8064516129032258No Hit
GAAGCAGGCTCTCCACTAAGTGGGGAGCCTGACGAGGGGCTCATTCCCAG10.8064516129032258No Hit
CCTGCGGGTGATGTGAAGCCTGTCGACAGCCCGCCTGTGTGCGGACCGTG10.8064516129032258No Hit
GAAGAGAAGGTCGTTTCAATCCCTGGATTCCAATTTCTGTTCTGCTCTGG10.8064516129032258No Hit
AGGAGACAGAGTGGCATTTTGTACAATATTAAATTCAGGTTCTTAATAAT10.8064516129032258No Hit
GAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTT10.8064516129032258No Hit
CTGGCTGGCTGTCAGTAAGTATGCTAGAGTCCCGGTTTCGTGTACTTTCC10.8064516129032258No Hit
GTTAGAAGGAGAGCAAATTCTTATTGAACTTTTGCATGTAAACTGGGCTG10.8064516129032258No Hit
CCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAGGGAAA10.8064516129032258No Hit
AATGAAGGGAGTGTACATAAATACGGCCTTGCTCAATGCATCCTGTGCAG10.8064516129032258No Hit
GCCCTGCAGTGGGACACTTCTGCTGTAAAATAGTTCCTCCTCATGCTTGC10.8064516129032258No Hit
GTCTCTAACAGTAGCAGGAAGCAGTAACGGTCTCAGGACTTGGGGTCCTA10.8064516129032258No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC10.8064516129032258No Hit
GGCCACAATTATTGCCTCAGCAATTGACTGCTAGTCTCTCCCGCGTAAGA10.8064516129032258No Hit
GGCGACGAGAGCATGAAGGCATTTGAGATCACCTGTGGGTTTAAAAAAAG10.8064516129032258No Hit
CAGCTTCTAAATCAACAAAGCAGAAACCTGATAAAATTCAAGGGAGCAAC10.8064516129032258No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC10.8064516129032258No Hit
GTCCTGGGCAAGCTCTTTCTCTGTTAATGCCTCAGTTTCCACACCTCAAA10.8064516129032258No Hit
AACATTGTCGATGGATTCAATTCCCCAGTTCTGGAAAATCACATTTTCAT10.8064516129032258No Hit
GTCCAGTGGGAATTTCCCAGTGGGAATCTTGTACTGCTTGGGTCTCCAGA10.8064516129032258No Hit
CTCTGGACCCTCTGGACCTAATACTCCCTGTTCTTTGTTTGGCTTGGGAG10.8064516129032258No Hit
ATTTCAAGAACACAGTATTTTTCCCATTTGTGTGGCTCCAGTCTCGGGTC10.8064516129032258No Hit
CCCCTAGATAACAGAAAATCATCTGTCATGTACCACCAAAAGTAACTTGC10.8064516129032258No Hit
CAACTGAAAGAACCCTAGAGAAGCGCTGACATCTTCAGGTTGTGGATTTG10.8064516129032258No Hit
GTCTTGCGGTGGAAAATACCACCAGGCCAGCTGGGGAGCTCTAACTCCCA10.8064516129032258No Hit
TTTCACAGAAAAGCTGATGCTGAAATTGTATTTAACATCTTTACTAATGA10.8064516129032258No Hit
GAGCAAGGCCGTATTTATGTACACTCCCTTCATTATGTATTCAGTAGCCC10.8064516129032258No Hit
GGCACAGACACAGGGCTCTCCCCAGCCACAGGGAAAGGCCACCAAAGGCA10.8064516129032258No Hit
CTCGTGACTCGGGTCTTCAATACTTAATTTCAGAGCATCCAACAGCAGGA10.8064516129032258No Hit
GCCCAGGATACACCATGGACCCAGTAAACAGCCCACACCAATCCTCAGCA10.8064516129032258No Hit
CTCATGCAGAGATAGCACTGTTTTGTATAGTTTTCCATTACATATGTCTA10.8064516129032258No Hit
ATGTATAGGATGTCCCCCCAAAAGGAGTTGGGTAGTCAGAATATTGTCAT10.8064516129032258No Hit
GTTCTGGTCCCATTCACACTCACGAGTACCCGTGCATTTTATGCAATTCC10.8064516129032258No Hit
ACATTATGTATTCAGTAGCACTGAAGTGGGACACTTCTGCTGTAAAATAG10.8064516129032258No Hit
CACTTGGGCTAACCCCCACGTCAGCAAACCCAGACTGAACAGCTAACCGA10.8064516129032258No Hit
GGAGGCTCTGAAATTAAGTATTGAAGACCCGAGTCACGAGGGGGAGGGCA10.8064516129032258No Hit
GTCTTGTTCTGCTCATGTAGGAGCATATTCTGGCTGTTAGGAAATGTCCA10.8064516129032258No Hit
TGAGATAACAAATCGGAAACATGGCCAATCGGAGAGTCACCCAGGGGAGT10.8064516129032258No Hit
GGAGTATGTTTCTGCTCGCTTAAGTAACCTCTACATCCAACACGGGGCTC10.8064516129032258No Hit
GTCAAGTTTTCTGCCCTCAAGGGGCTCAGAGTCCTACTAGGGTACTGCTC10.8064516129032258No Hit
GAATATACATGATTTTATTTTTGGATCAATCAAGTTGCAGACCTCACGAC10.8064516129032258No Hit
ACAGGAGATTGTACCCATGAAAAAGGAGCAGTCAGACATCAGGAAAGACT10.8064516129032258No Hit
GCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATC10.8064516129032258No Hit
GGGTCATCCACAACCCTAGGCAAAACAATAATGGCCGTGTTAGCGAGCCA10.8064516129032258No Hit
GCTTGGTCCATGAGCATGTCTGGTTTTACAACTCTTGTCAGAGGCTCAGA10.8064516129032258No Hit
CTACAAGGACAGTCATGAGGACAGACTGCAGGAGGCCTGGGACTACGTGC10.8064516129032258No Hit
TAACTAAGCTAATAAATTTCAGGCTTAGTTCTCTCATAGAAACATTTCTT10.8064516129032258No Hit
CTATTGAAGCACCGATTTGAGATAATTGAAGGAAGAGACCGAATCATGGC10.8064516129032258No Hit
GAAGCAATTTTCTAGATTCAGCCGAAAACCCCTCAAGTTGTGGAGACGCA10.8064516129032258No Hit
TCCCCTCACAGGGCATGGTAGGGGAGAACAACTAAGAAAAGGCGAAAGGG10.8064516129032258No Hit
CACTGTAGGGTGATACCAAAGGTCTATGTGATAATTTTTGTTACGATAAG10.8064516129032258No Hit
CCCTCAGATTGCCTGATGGGCCTCTTTGCCATCAGCGGTCAAAGTTCCTG10.8064516129032258No Hit
ACTTAGGTGTGACTTCAATGCTTGTGTCTAAGCAAGGCTAACTGGGGAAG10.8064516129032258No Hit
ATTTCGCAAATTTAGAACGGGTGCCCCATTGTAAAGTAGGCACTGTCCCA10.8064516129032258No Hit
GAGTAAAAGAATGAAGATTCGAACACAAATACCAGCAGAAATGCTAGCAA10.8064516129032258No Hit
TTCTAGTTCTGTGAGAAGTGTTATTGGTATTTTGATAGGTATTACATCAA10.8064516129032258No Hit
ATATTAACTTTACTTGATTTTGCTGTCTCGTTAACTTGTATAGCCAAGAG10.8064516129032258No Hit
GGCCACTGCTGCGGTAAAATAGTTCCTCCTCATGCTGTCGAGATGTTCCA10.8064516129032258No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG10.8064516129032258No Hit
AAGTAGGGCTCTGCTTTCCATTTCTGACCTGGGAAGAACCAACAGGGGAG10.8064516129032258No Hit
GAGGAATGATGATATCCGACCACATGGCAGAGCGGAGATGGGCTGTGGGC10.8064516129032258No Hit
CTACCACTCTCTCCGTGCTGGAGTATTCATCTACTCCCATTTTGCTGACT10.8064516129032258No Hit
ATTGAAATTGGAGTAACACGGAGGGAAGTCCACATATATTACCTAGAGAA10.8064516129032258No Hit
GTAATCTCAAATTTTTCTTCAATTGTCTATTCGCCTCTTTCTGACTGACG10.8064516129032258No Hit
GCAGGAATAGGAGCAGGAGCAGGTGCAGGTGCTGGAACAGGTGCAAGAGC10.8064516129032258No Hit
ACATAAAATCGTAACCATTTTTAAATGTGCAGTTCCATGGCATTAAGGAT10.8064516129032258No Hit
GCTTCAGGACAGGGGAGGCGGTCCCCTGCTGTAGACTCTGGTTTCTGCTC10.8064516129032258No Hit
ATTGTTGGCTTCGAGTCCCGTCTCCCAGTGGGGCTTGCTGGAGGTGTTAA10.8064516129032258No Hit
CTATTCCTAGACTATATAGTGTTTTTATTATGAAAGTGTTAGATTTTGTC10.8064516129032258No Hit
CCACAAAGCAGAGCCCAAAAATAGTGACACTCAGGGAAGCTCCACGGGCA10.8064516129032258No Hit
CTCCAAAGCTGGGTAGCTCCATGCTAAAATTAGCCACAAATCCATAGCGA10.8064516129032258No Hit
AAAAAAAGGAAGACACGGTTAATAGAGTCTATTTTACACTTGGGCCTGTG10.8064516129032258No Hit
GCGTAATGCTGGGCCATATCTCTGGGCTTATTTGCCCAAAATGAGAAATC10.8064516129032258No Hit
CAATTGAAGAGCCATCTGGGCCGTTGCAGGTCCAAGGTCATTGTTTATCA10.8064516129032258No Hit
AGTTAGATTTCCTGTCTTTGCATGTCTGAAAAGATCTTTATTCCATCCTT10.8064516129032258No Hit
CCCCAATACTCAGCAGCAGGGAAATGAGGATCTTGGAAACAGACAGCACC10.8064516129032258No Hit
TCTGGAAGCAGGTCCAGATAACACATGGCGTTAGCAGACATCTTCTGTCG10.8064516129032258No Hit
TCTTTGGTCCTACATTTGCTTATCATTGGGATCAGCTGAAAGTCATCCAT10.8064516129032258No Hit
CTGGTCAGATGACGGCTCTGGTGGGGCCCAATGGGTCCGGGAAGACCAGA10.8064516129032258No Hit
GGTTAGGTCCAAGTGGAATAGTGGCAGGGTGAGTCCCCAGGTGGGGATGG10.8064516129032258No Hit
GCCATGGAACAGGAACAGGATACACCATGGACACAGAGACTGGTGCCCCC10.8064516129032258No Hit
CTCTACATTCATTCTGTCCCCTGTGCACAGAACCTTTGTAGAAGCTCTTT10.8064516129032258No Hit
AAAGCAGGATGGGAAATCAGGATGCAGACACTGGCCAAACACAGTGTGGG10.8064516129032258No Hit
CAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGA10.8064516129032258No Hit
GATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACA10.8064516129032258No Hit
GTACATCACAAATGACGTGTGAAACTAAGGAGTTCAGCCGCGGCGATACC10.8064516129032258No Hit
GATCCTGGGATCATGACCTGAGCCAAAGACAGAAGCTTAATGACTGAGTC10.8064516129032258No Hit
ACGTTCCATGGGGCCAAGGAGGTGTCACTAAGCTATTCAACTGGTGCACT10.8064516129032258No Hit
TGCAAGGTGAGATTCTGGAAGGCCCAACCAACAGCGGTGCCCGGAGGGTT10.8064516129032258No Hit
GTCAAAACACTGCTTACAACTTAGGACTGGGCTATACATCCAACAGCAGC10.8064516129032258No Hit
CGATTACTGTGTGTGTCGTGCTACATTAAATGTTCCCTCATTCGATGTGT10.8064516129032258No Hit
GATGCAGAGAGAGGCAAGTTAAAAAGAAGGGCTATCGCAACCCCGGGGAT10.8064516129032258No Hit
GCCGCGAAGTCGACCCTGGCATCAATCCGGGCACGCGACAACATGGCCTA10.8064516129032258No Hit
CTGGAAGTTAAGGTGATGACCCAACTACTTTGGGCTTCTCATGCCTCTAA10.8064516129032258No Hit
GCCCATGGCTTCATACAGGAGTTGGAGCACGGACTGAGTACAGATGTAGG10.8064516129032258No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC10.8064516129032258No Hit
CCCTGGAAGTGCCACCCTCCTCAGGAAAGACAGGGTCACCCACAAGTGTC10.8064516129032258No Hit
AGGGTGTAGTCCGCTTTGGTGGCCATCTCCTCTCCAGTGAATGAAAAGAT10.8064516129032258No Hit
CATTGTCGATGGATTCAATTCCCCAGTTCTGGAAAAGCACTTTTGCATCT10.8064516129032258No Hit
ATTTTACAGCAGAAGTGTCCCACTGCAGGGCTACTGAATACATAATGAAG10.8064516129032258No Hit
GAGTGGGAGAGGGAGACGCAGGCTTCACTCTGAGCAGGCAGCCGGATGCA10.8064516129032258No Hit
GTTTTGGTGGGTTCACTTTCAAAAGGACAAGCGGATCATCAGTCAAGAAA10.8064516129032258No Hit
CTGCCCGACATGACCCCAAGCACGGAGCTGTCGCTGAGAGGGATAAGAGT10.8064516129032258No Hit
GATCTGACGTGTCTCCCTTCCCAGTATCACCCCCACCATGCAGGTCCTTC10.8064516129032258No Hit
GCCCTAGACACCATGGCCTCCACATCTGTTCATCCTCAAGAATTCCCCTT10.8064516129032258No Hit
GACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGAC10.8064516129032258No Hit
GTTATTAAGTTCCGAAGCTCCTCCTTTACCTGGAAAGCTTTGATATATCT10.8064516129032258No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers