FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R2_A03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R2_A03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGAGCAGAGACTGGAAAGTGTATTGGCAGGAAAGAACACAGCTCGT13.4482758620689653No Hit
CTTATCCCTCTCAGCGACATCTCCGTCCTTGGATTCATGTCGGGCAGTAT13.4482758620689653No Hit
GTGTTGCGATAGCCCTTCTTTTTAACTTGCATCGCGCTGCATCTTTGTGC13.4482758620689653No Hit
GTCCCGCACTCGCGAGCTACTCACTAAGACCACTGTGGAGCATATGGCCT13.4482758620689653No Hit
TTTTGGACCCAGCTTGCTAGAGATCTGGGCTCTGGCTCATCACTGTCATA13.4482758620689653No Hit
AAATGTCCCTGTCTTATTTATATAGGACTTCTTTTTGCTCATGTTGCTTC13.4482758620689653No Hit
CGACTGACCACGGGACACCTGCGGTCTCCTGGCCGGGCCTGCAGGGCAGC13.4482758620689653No Hit
TGCCAGCTGTGACTCTGATGTCAGTATTCTTGCACCCACTTCATTTGGGA13.4482758620689653No Hit
TTGCATGGGCCTCATATACAACAGGATGGGAACAGTGACCACAGAAGCTG13.4482758620689653No Hit
GACTTGTATCTGACCCTTTCTTTGGAAAGCAGAACCAGAGACAAGATTAG13.4482758620689653No Hit
TGGTATACTCACAGGAGGATTGCATGATCAAGGCAGTTAGGGGCGATCTG13.4482758620689653No Hit
GGTTAAGAGTCATCAAAATGGTAGTATATGAATACTCCAGCACGGATATA13.4482758620689653No Hit
AACATATACTGTTCACCACTGTCCAGGCAATGATACGGTCTATTCCTACA13.4482758620689653No Hit
GGTCGCATGTGAGACCTGATACTGAGGTGGTGAACTTTGTAAGGCTGGAG13.4482758620689653No Hit
ATTATGATCATATTGATTACCAAAATGATAAAATCAACGTAATCTTAACA13.4482758620689653No Hit
GTATCATTTGAGAAATTATAATTATGTTTTTAAATTTGTAAGTATGTAGT13.4482758620689653No Hit
ATTACATCCATTAAGAAATCTATTAGCCTTCCTGACTCATTAGCTGTTAG13.4482758620689653No Hit
GGAGGCACAGGATGTGATTATGGAAGTTGTTTTCCCAAATGAAGTGTGGG13.4482758620689653No Hit
AAACAAAGCAACCAACGAGATACAAACAAATGCAGCGAACTGCACAGGTC13.4482758620689653No Hit
CAATTATTGCCTCAGCAATTGACTGCTCGTCTCTCCCGCTTACTATCAAC13.4482758620689653No Hit
TTGTAATTGAATACTGGAGAATTGCCTCTTACCAGTATCCTCAACCCTGA13.4482758620689653No Hit
GGCTGAGCCAATCCATACCGGTTAGTTAGTGATTAGTCAAAACACGAAAT13.4482758620689653No Hit
CCTCAGAATGAGTGCTGACCGTGCCAGGAAAATGAGGTCTTCAATCTCAG13.4482758620689653No Hit
AGCGAAAGCAGGGAGGGCAGTCAGGAGGCAGCTCTAGTAATCCATGCAAG13.4482758620689653No Hit
CATCGACATAGGCTCTAAAGTTTTCAAGGCTGGGGAAGTTCGGTGGGAGC13.4482758620689653No Hit
GCTCATCCCTATGCAAAACAAAATAGGCCCTAAATCCGTGAGACTGCACC13.4482758620689653No Hit
ACTTCCCAATGTGATTTGGAATACCTGAATGTAGCTGATTTGGTATGATT13.4482758620689653No Hit
AGTTAAAATAAGGAGGAGAGTTGATACAAACCCTGGACATGCAGATCTCA13.4482758620689653No Hit
CATAATCAACTGAGATACCCAGGTGCTTCAATTCTACCTATTCTTTCATT13.4482758620689653No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)