FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R2_A02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R2_A02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1248
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT40.3205128205128205No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG40.3205128205128205No Hit
GTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATTTCAGA30.2403846153846154No Hit
GTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCA30.2403846153846154No Hit
GGTGCTAGCAGAGCTACAGGACATTGAAAATGAAGAGAAGATCCCAAGGA30.2403846153846154No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT30.2403846153846154No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT30.2403846153846154No Hit
TCTTTGCAGTCATCAAAGTCTACTTTTTCTGGTGCCATATTTTCACCGAG20.16025641025641024No Hit
GAATACCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGG20.16025641025641024No Hit
GAGAAATAACGTTCCATGGGGCCAAGGAGGTGTCACTAAGCTATTCAACT20.16025641025641024No Hit
ACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACAT20.16025641025641024No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG20.16025641025641024No Hit
GTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTTGA20.16025641025641024No Hit
CAGTGATAAAGAACAACATGATAAACAATGACCTTGGACCTGCAACGGCC20.16025641025641024No Hit
GTCCTCAAGAGCATGTCTCCTATTTCAAGAACACAGTATTTTTCCCATTT20.16025641025641024No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG20.16025641025641024No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG20.16025641025641024No Hit
GTATGGGTTCATTATAAAAGGAAGGTCTCATTTGAGAAATGATACTGATG20.16025641025641024No Hit
GTGTTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT20.16025641025641024No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT20.16025641025641024No Hit
CTACAGGACCTGCAAGTTAGTGGGAATCAACATGAGCAAAAAGAAGTCCT20.16025641025641024No Hit
GGGCCAAGGAGGTGTCACTAAGCTATTCAACTGGTGCACTTGCCAGTTGC20.16025641025641024No Hit
GTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATCA20.16025641025641024No Hit
ACTCCAGACACTCCAAAGCTGGGTAGCTCCATGCTAAAATTAGCCACAAA20.16025641025641024No Hit
GGGCAATGAAAAGAAGGCCAAACTGGCAAATGTTGTGAGAAAGATGATGA20.16025641025641024No Hit
GTAAGGTCCTGCACACTTTCCCAATAGAGCCTTCCTCCACTCCCCTGGGT20.16025641025641024No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG20.16025641025641024No Hit
GGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGGAATTTTAGGA20.16025641025641024No Hit
CTCTTGAGGCATTTCCAAAAAGATGCAAAAGTGCTTTTCCAGAACTGGGG20.16025641025641024No Hit
ACACTAAGTGGAATGAAAATCAAAATCCTCGAATGTTCCTGGCGATGATT20.16025641025641024No Hit
GCAGATGTACACTCCAGGAGGAGAAGTGAGAAATGATGATGTTGACCAAA20.16025641025641024No Hit
CTCCCAAGACCGTACTTAGCATGTTGAACATGCCCATCATCATCCCAGGA20.16025641025641024No Hit
GCCATGGATGACTTTCAGCTGATCCCAATGATAAGCAAATGTAGGACCAA20.16025641025641024No Hit
GTAGCCATCTGTCTGTGAGACCGATGCTGTGAATCAGCAATCTGTTCACA20.16025641025641024No Hit
ATGTAATCATCGCCAGGAACATTCGAGGATTTTGATTTTCATTCCACTTA20.16025641025641024No Hit
AGAAAGCTCTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGC20.16025641025641024No Hit
CTATCAACTGGATCAATCTCCTGGTTGCCTTTCTGAGAATAGCTGTTGCT20.16025641025641024No Hit
GTTGAGAATAGAACGATTCCTCTTGGGAATCCAGGAATGTGTAGTTGCAA20.16025641025641024No Hit
ACCCTGATCCTCTCACATTCACAGTCAATGAGGAAAATTGCATCCTACTC20.16025641025641024No Hit
AATCCTGCCTGCCTGCTTGTGTGTATGGGCTTGCAGTAGCAAGTGGGCAT20.16025641025641024No Hit
CCTCATGCTTGCGATATGTTCAATCGGGGCAACATCTTCTCCTATTTCAT20.16025641025641024No Hit
GCGGTCAAAGTTCCTGCTGATGGATGCTCTGAAATTAAGTATTGAAGACC20.16025641025641024No Hit
GTCTCGGGTCAGTGAGTGAGAACTCCATACTTACAAAGTTCACCACATCA20.16025641025641024No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT20.16025641025641024No Hit
AGCAAGCATTGACCTGAAGTACTTCAATGAATCAACAAAGAAGAAAATTG20.16025641025641024No Hit
TCTGAGAATAGCTGTTGCTCTTCTCCCAACCATTGTGAATTCTTCATACC20.16025641025641024No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC20.16025641025641024No Hit
ATTTCAGAGCATCCATCAGCAGGAACTTTGCCCGCTGATGGCAAAGAGGC20.16025641025641024No Hit
AAACTATTCTCTGTCGCTTTCAATCTGTGCCGCATTTCTTCAATTAACCA20.16025641025641024No Hit
CTTCAGGTCGGCTGCATATCCTGACCCCTGCTCATTTTGATGGTGATAAC20.16025641025641024No Hit
AGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCATGTTCAACA20.16025641025641024No Hit
CTGAATACATAATGAAGGGAGTGTACATAAATACGGCCTTGCTCAATGCA20.16025641025641024No Hit
TCTTTGCCATCAGCGGTCAAAGTTCCTGCTGATGGATGCTCTGAAATTAA20.16025641025641024No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT20.16025641025641024No Hit
GTGCTAACGGGCAACCTCCAAACACTGAAAATAAGAGTACATGAAGGGTA20.16025641025641024No Hit
CGCTCACCGTGCCCAGTGAGCGAGGACTGCAGCGTAGACGCTTTGTCCAA20.16025641025641024No Hit
GTCCTGGGATGATGATGGGCATGTTCAACATGCTAAGTACGGTCTTGGGA20.16025641025641024No Hit
TGCTGACACTGCTGCTCTTCTTACTATGTTTCTAGCAGCGATAATCAAAC20.16025641025641024No Hit
CAAGTCCTCCGATGAGGACCCCAACTGCATTTTTGACATCCTCATAAGTA20.16025641025641024No Hit
GTGTTTATATTGAAGTGTTGCACTTAACCCAAGGGACGTGCTGGGAGCAG20.16025641025641024No Hit
GCCTTGCATATGTCTACGGCTTGTTCCTCAGTTGGATTCTGTCTAAGGAT20.16025641025641024No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)