FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R2_A01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R2_A01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT14.761904761904762No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG14.761904761904762No Hit
CAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGTGTGTG14.761904761904762No Hit
ACCGTACTTAGCATGTTGAACATGCCCATCATCATCCCAGGACTCAGTGA14.761904761904762No Hit
TCTCCTGTCTTCTTTGACCAAAATGAGAAATCCGCTCAGGCCAGCAGACT14.761904761904762No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG14.761904761904762No Hit
TAGTTTTGATTCTTGCCCTGCTCTCTTCGTCAAGGGTGTAGTCCGCTTTG14.761904761904762No Hit
GAGTCTATGGGATTCCTTTCGTCAGTCCGAAAGAGGCGAAGAGACAATTG14.761904761904762No Hit
TCTTGACTGATGATCCGCTTGTCCTTTTGAAAGTGAACCCACCAAAACTG14.761904761904762No Hit
GACAAGAAATGGCCAGTAGGAGTCTATGGGATTCCTTTCGTCAGTCCGAA14.761904761904762No Hit
ATGATAAACAATGACCTTGGACCTGCAACGGCCCAGATGGCTCTTCAATT14.761904761904762No Hit
AAACTATTCTCTGTCGCTTTCAATCTGTGCCGCATTTCTTCAATTAACCA14.761904761904762No Hit
GGTCCTGTAGAATCTGTCCACTCCTGCTTGTATTCCCTCATGGTTTGGTG14.761904761904762No Hit
GGATATGCAGCCGACCTGAAGAGCACACAGAATGCCATTGACGAGATTAC14.761904761904762No Hit
AGGAAAATGAGGTCTTCAATCTCAGCGTTTCCTGGGTTTCGACTTTCTCT14.761904761904762No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT14.761904761904762No Hit
ACCTCATGGCTTGGAAGCAGGTGCTAGCAGAGCTACAGGACATTGAAAAT14.761904761904762No Hit
GAAGAAGTCAGTGAAACGCAAGGAACTGAGAAGTTGACAATAACTTATTC14.761904761904762No Hit
CAAAGAGGGAACGTACTATTGTCTCCCGAAGAAGTCAGTGAAACGCAAGG14.761904761904762No Hit
GTGCCGCATTTCTTCAATTAACCACCTTATTTCCTCAAATTTCTGTCCCA14.761904761904762No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG14.761904761904762No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)