FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R1_H09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R1_H09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATGTAATCTTAGCCAGGAACATTCGATGATTTTGATTTTCATTCCAATT11.3513513513513513No Hit
TCAATGAATCAACAAAGAAGAAAATTGAGAAAATAAGGCCTCTTCTAATA11.3513513513513513No Hit
GTCCCTAAGTGACTGAACAATGAGAAGAAATTTTCTAGATTCAGCCGAAA11.3513513513513513No Hit
CGATAAATACATTACAAAAAAAGAAGATCAGTAATCAACAATATCAGATG11.3513513513513513No Hit
CCAACACCATGTCAAGCTTTCAGGTAGCCTGTTTCATTTCGCATATCCGC11.3513513513513513No Hit
GTTGTTATATTTAAGTGTTGAACTTAACACACGGCACGTGCTGGGATCAG11.3513513513513513No Hit
GAATCAAAGACCGAAATTTCTGGAGGGGTGAAAATGGAGGAAGGACAAGG11.3513513513513513No Hit
ATAAAGAACAACATGATAAACAATGACCTTGGACCTGCAACGGCCCAGAT11.3513513513513513No Hit
GGAGGTAATGGAATCAAGGAACAAATAGGAAATAGAGCTAACAACCCACT11.3513513513513513No Hit
ATTCAGCCGAAAACCCCTCAAGTTGTGGAGACGCATATAGACTGTTGAAT11.3513513513513513No Hit
GAATGGAGGAGTAAGGATGGTGGACAACCTTAGACAGAATCCAACTGAGG11.3513513513513513No Hit
CAACAGTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCTA11.3513513513513513No Hit
CAATAGGGTTGAGGATTAGCTCATCTTTGAGTATTGGTTGGTTAACTATC11.3513513513513513No Hit
TTCCTGACTCATTAGCTGTTAGGCCATTCGATCTAAAGACTTCTATGGTG11.3513513513513513No Hit
TCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCGTTGCCTTTTCGTC11.3513513513513513No Hit
TCATGGATTGGTGAAATCACTATGAGAGCAAAATCGTCGGATCAAAGGAG11.3513513513513513No Hit
GGACAACACTAAGTGGAATGAAAATCAAAATCCTCGAATGTTCCTGGCGA11.3513513513513513No Hit
CAAGAGAATAATGGACATGATTCCAGAGAGGAATGAACAAGGACAAACCC11.3513513513513513No Hit
ACTTATGATTGGACATTAAACAGAAATCAACCGGCAGCAACTGCATTGGC11.3513513513513513No Hit
ACTCATGTCAAAGGAAGGCACGATCGGGTTCGTTGCCTTTTCGTCCGAGA11.3513513513513513No Hit
AGGGGGAGGGATTACAACTATATGATGCAATCAAATGCATGAAGACATTC11.3513513513513513No Hit
GTGGTAATGCCAGCCCATGGTCCAGCCAAAAGCATGGAATATGATGCCGT11.3513513513513513No Hit
TTATAAAAGGAAGGTCTCATTTGAGAAATGATACTTATGTGGTGAACTTT11.3513513513513513No Hit
TGTCAAAGGAAGGCACGATCGGGTTCGTTGCCTTTTCGTCCGAGAGCTCG11.3513513513513513No Hit
CTTCATTTGGGAAAACAACTTCAATAATCACATCCTGTGCCTCCTTGGCA11.3513513513513513No Hit
GTATCAGATGGAGGGCCAAACTTATACAATATACGGAATCTTCACATTCC11.3513513513513513No Hit
GTTCACTTTCAAAAGGACAAGCGGATCATCAGTCAAGAAAGAAGAAGAAG11.3513513513513513No Hit
GGCATATCCGCAAGCGATTTGCAGACAATGGATTGGGTGATGACCCATTC11.3513513513513513No Hit
ACTCGGGTTGATTTCTGGTGATATATGTAATCATCGCCAGGAACAGTCGA11.3513513513513513No Hit
TAATGTCAGTGCTCTTATTAGATAGCCTCTATTATTCAGTCTTTCTTTTT11.3513513513513513No Hit
CTATTCAACTGGTGCACTTCCCAGTTGCATGGGCCTCATATCCAACAGGA11.3513513513513513No Hit
GTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCT11.3513513513513513No Hit
GGAAAAATACTGTGTTCTTGAAATAGGAGACATGCTCTTGAGGACTGCGA11.3513513513513513No Hit
GAGGAAAATTGCATCCTACTCTGTTCTGGTGGGGCAGCAGCAAAGGGGAG11.3513513513513513No Hit
CAGGAAGGCTAATAGATTTCTTAAAGGATGTAATGGAATCAATGAACAAA11.3513513513513513No Hit
GTTCAACAAGCTAAGTACGGTCTTGGGAGTCTCGATACTGAATCTTGGAC11.3513513513513513No Hit
GTGAAAATATGGCACCAGAAAAAGTAGACTTTGATGACTGCAAAGATGTT11.3513513513513513No Hit
GGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGTGTGTGCCACT11.3513513513513513No Hit
ATATAATCATCGCAAGCAACATACCAGGATTTTGATTTTGTTTCCAAATA11.3513513513513513No Hit
CATAGTAAGAAGAGCAGCAGTGTCAGCAGACCCATTAGCATCTCTCTTGG11.3513513513513513No Hit
AACCCAGGAAACGCTGAGATTGAAGACCTCATTTTCCTGGCACGGTCAGC11.3513513513513513No Hit
CCTTCTGGGAGCCGGCACTGTTCTCGCTGGTGGGTTTTGCCGGTAGCTAA11.3513513513513513No Hit
CATTGAAGTACTTCACGTCAATGCTTGCTAGCATATCTGCTGGTATTTGT11.3513513513513513No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT11.3513513513513513No Hit
CCAGAACAGAGTAGGATGCAATTTTCCTCATTGACTGTGAATGTGAGAGG11.3513513513513513No Hit
CACATATATGAGCCATAGGGGAGACACACAAATTCAGACGAGAAGAAAAT11.3513513513513513No Hit
GTCGTGCTCTTTTTGGCGCCAGAGGAGACCCGCTGTCTGGGTGAGTTTGG11.3513513513513513No Hit
TGTTTCCGATTTGTTTTCAAAGAATTCCTTGGTCATGTCTTTCTCTTTGA11.3513513513513513No Hit
GAAGAGGCAACACCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGG11.3513513513513513No Hit
GCGTGATGCCCCATTCCTTGATCGGCGCCGCCGAGATCAAAAGTCCTTAA11.3513513513513513No Hit
TTCACTATGAGAGCAAAATCGTCGGATGATTGGAGCCCATCCCACCAGTA11.3513513513513513No Hit
GTGCAGGAGCAGGTGCTGGAGCCGGAGCAGGGGCCGGAGTCTTTGCGGGG11.3513513513513513No Hit
AACATCCACAGCACTCTGCTGTTCCTGTTGATATTATTCCCTCATGGACT11.3513513513513513No Hit
ATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATCATGCG11.3513513513513513No Hit
ATTTAAAGTTTCAATATTACCAAAAGAACAAAGAGTTAAAAAGTGGGAGA11.3513513513513513No Hit
TGCTGGAACAGTGTCCTTACGAATCCACTGTACCGGCTTCTGGTTGCCTT11.3513513513513513No Hit
GTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCA11.3513513513513513No Hit
GCTGTTCCTGTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCGTA11.3513513513513513No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG11.3513513513513513No Hit
CTGATTCACAGCATCGGTCTCACAGACAGATGGCTACTACCACCAATCCA11.3513513513513513No Hit
GGCTTGCATAAATGTTATTTCTTCGAAAATATTCTCTGTCGCTTTCAATC11.3513513513513513No Hit
TTCCAGGTTCCAGGTTGTCCCTAAGTGCCTGAACAATGAGAAGCAATTTT11.3513513513513513No Hit
GGTCAATGCTTGCTAGCATGTCTGCTGTTATTTGTGTTCGAATCTTCATT11.3513513513513513No Hit
GATCAAGTAAGAGAAAGTCGAAACCCAGGAAACGCTGAGATTGAAGACCT11.3513513513513513No Hit
CTATTAGCCTTCCTGCCTCATTAGCTGTTAGCACATTCGATCTAAAGACT11.3513513513513513No Hit
CGGTCAAAGTTCCTGCTGATGGATGCTCTGAAATTAAGTATTCAAGCCCC11.3513513513513513No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC11.3513513513513513No Hit
GACTAGAGACGCTCACTGCTACCTGCATCTTTCCTGTTTCCCCTCTGCTC11.3513513513513513No Hit
TCCCACCAGTATATTGTCTTGTTGTATTTCTTTTGTACAAGATTAAGTAT11.3513513513513513No Hit
CTTCTCCTCCTGGAGTGTACATCTGCTCCCACCCCGTCCCTTGGGTTAAG11.3513513513513513No Hit
ATACAGTAAAGACAACAGAGTAAGAATCGGTTCCAAGGGTGATGTGTTTG11.3513513513513513No Hit
ATTTTTAAGGGTTAGAGATCAAAGAGGGAACGTACTATTGTCTCCCGAAG11.3513513513513513No Hit
CTTTATCACTGTTACTCCAATACTCATGTCAGCTGATTCATTTACTCCAG11.3513513513513513No Hit
GAGTAAGAGACAACATGACCAAGAAGATGGTCACGCAAAGAACAATAGGG11.3513513513513513No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCACC50.0145.01
CCAGTAT50.0145.06
CAGTATA50.0145.07
TTATCAA50.0145.0145
CCCACCA50.0145.02
GTATATT50.0145.09
AGTATAT50.0145.08
CCACCAG50.0145.03
CACCAGT50.0145.04
ACCAGTA50.0145.05
Produced by FastQC (version 0.11.5)