FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R1_H07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R1_H07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1344
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATAGCTAAAGTGCCAGGAGAAATGACTAGGAAGGTGGGAAACCCTC30.2232142857142857No Hit
CCCCTGCACCACGGGGAGGGCCACCCAGCTGCACATAGGGAGAGTGAGTG30.2232142857142857No Hit
AACCTATACAGATTATCAGAACTAATCAGAAACATCAGGAAAACCACTGA30.2232142857142857No Hit
TTTTTATAGCGAACAGAGCTCTCCCATCAGGACTAAGAGATAAGTGGTAG30.2232142857142857No Hit
GTCAGAGGCTGCAAAGTCAGATCGAGAACCTCAAGAAGCAGAATGCCGAC30.2232142857142857No Hit
GGTGTGTACAGAGTCGGGGAGATCGGGGAGAACAGCGGGGTTTTCCAGAG30.2232142857142857No Hit
GATTTATGAGGGGAGGGGACAAGGTCAGCAGCTGCTCGAGAGACTCTTTG20.1488095238095238No Hit
GCCCCAGAGGGAGGCAGATGGCAAGAAACTGAGGGGGCCCAAGAGCGGTC20.1488095238095238No Hit
GGGTCGGGCACTGTGATTTATGAGGGGAGGGGACAAGGTCAGCAGCTGCT20.1488095238095238No Hit
CTTCTGTCCCAGCAAGGACGTCATCCACTGGGCCCTCTGGCATCCTCTGA20.1488095238095238No Hit
TCCCAGGACCCTGGGATCATGACCTGAGCCGGAGGCAGACGCTCAACCGA20.1488095238095238No Hit
GTATTTGGCCAGCTGTGTATGCAGCTCAGGGCTCTCATTCATTAGGTTGA20.1488095238095238No Hit
TTATGAGGGGAGGGGACAAGGTCAGCAGCTGCTCGAGAGACTCTTTGCTT20.1488095238095238No Hit
AACGTATTCACCGCAGCGTTGCTGATCTGCGATTACTAGCGACTCCGACT20.1488095238095238No Hit
GCTTATACACTTGTAAATAAGACTCAAACCCAGATTTTTTTGCCCAAAAT20.1488095238095238No Hit
GGATATATGGAAGACACGACTGGGAAACAGTTCTTGTAAATGACTAAAAT20.1488095238095238No Hit
AGGTAGCGGAGAACCCTTTCCTGCTCCTCTCCAGAGCGGTTCATGAAGTC20.1488095238095238No Hit
AAGCAGGGGAGGGACAGCAAGAGAGGGAGACGCTCCTCGCTGAGCAGGGA20.1488095238095238No Hit
GCTTATAACCCAAGAGCACATTTCATCAACAAACGTTCATGGCGACACCC20.1488095238095238No Hit
GTAAGTACTCAATAAATGTCAGGTCTTCGTATTTTGTAGAAATGGAGACA20.1488095238095238No Hit
ACCCAAACTGTGGTATGGAAGAGTTAATGTTGGCAGAAGGCAGTTCACCG20.1488095238095238No Hit
ATACAATGTCTCCTTGCCTTCTTTTTCTACAGATCTGTCGCTGGGCGGTT20.1488095238095238No Hit
CTTAGATTGATAGTCAGTGCTATCACTATTTCAATGAAATTACTACTACT20.1488095238095238No Hit
GGTCCGCACTGGCTGAGATGAGGTCCATGTACTGGCAGATGGCGTGAAGC20.1488095238095238No Hit
GGCTTGACATGTTCTCGATCGCCGTAGAGATACGGTTTCCCCTTTGGGGC20.1488095238095238No Hit
TACCTAGATAGTGTAAACCCAGAGGCAGCTTCCTGGGAGCCACTGGGAGA20.1488095238095238No Hit
TTATAAGACACTGAATCACTCTTCAATTCTAGTGTAACTTTTCCTGAGAA20.1488095238095238No Hit
AACATGGACTCTGGCCTTCACAAAGCAGCCTTCACTGGCCATGGTCGGAT20.1488095238095238No Hit
GCAGGGAGGCCCCCGCACAGGGGCTGGGACCATGGTTTTAGGATGGGGAG20.1488095238095238No Hit
CAGGAAGGGCGTGTGCTGGTTGTCACCAGAGTCTTCCCCTCTGGTCAAGT20.1488095238095238No Hit
GGCTGAGATGAGGTCCATGTACTGGCAGATGGCGTGAAGCAGGAGCCTCT20.1488095238095238No Hit
ATATTTTCCTGATACATTTTATTCTTCCTCTTCCTCCAAGTATGTTTACT20.1488095238095238No Hit
GTTTATATGAGAAAGGAGGGGAATGAAAGAGAAAATAGAATCAAAACACC20.1488095238095238No Hit
GTCCATGTACTGGCAGATGGCGTGAAGCAGGAGCCTCTCGAAACTGCTGT20.1488095238095238No Hit
GTTCAAAATGGAGCCTCAGAAAAAGAGGTACTAGTTTTCAATCTTTGTTT20.1488095238095238No Hit
GTCCGACCCTGGGCCTCATGGCTGACATTCCAGGCACGTTTCCCAGCCCT20.1488095238095238No Hit
GGGAAAAAGAGAAACATCTCAAATTATATATTTGACAAACAATGATCTGA20.1488095238095238No Hit
GTACCATTACGCACCCCAGGTCCTTCTAGAATCACGCCCTGGAGGAGCCA20.1488095238095238No Hit
GTTGTCCAGAGAAAGAAAACAGGGTTGGGCATTTGTGAGGGACCTGCCCC20.1488095238095238No Hit
TTTCCCAGCTGCATCTCTCAGAAAGTGCGACAAGCATGGAGAACACCCCA20.1488095238095238No Hit
TTCTTGTAGCCGATGGCTACACTTTTGTAAAACAGTGTCGTAGACGCTCT20.1488095238095238No Hit
CCTTCCCGCCATCCAAAAGAAAGGAAAAAAAGAAAAGCAAGAGCAGCACT20.1488095238095238No Hit
CATTATGTTATAAATACCTGAGAAAAGAAAATCAGAGCTGTCCCAAAGAA20.1488095238095238No Hit
GCCCCCGCACAGGGGCTGGGACCATGGTTTTAGGATGGGGAGACCCAGAC20.1488095238095238No Hit
CTGCCCGCCTCCCCCCACCGCTTCCCATTCCTGTGAGGTTCAAGACCCAG20.1488095238095238No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)