FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R1_H05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R1_H05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGA17.6923076923076925No Hit
GCACACAGTATTTTTCCCATTTGTGTGGCTCCAGTCTCGGGTCAGTGAGT17.6923076923076925No Hit
GTTTCCTCATCCACCTTAGAATAGAGATGTTCAATTTTCAAAAGCAAACA17.6923076923076925No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG17.6923076923076925No Hit
TGCCCATCATCATCCCAGGACTCACTGATGCTGTGACATCTATTAGAAGA17.6923076923076925No Hit
CTATGTTGACAAAATGACCATCGTCAACATCCACCGCACTATGCTGTTCC17.6923076923076925No Hit
ATACAACAAAATGGAATTTGAACCATTTCAGTCTCTTGTCCCTAAGGCAA17.6923076923076925No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT17.6923076923076925No Hit
CAATAGTGCATTCGGGTCACCAGATTCTACAATTATTGATTCACCCCGTT17.6923076923076925No Hit
GAGTCAGGAAGGCTAATAGATTTCTTAAAGGATGTAATGGAATCAATGAA17.6923076923076925No Hit
ATATGTGTATCTGTAGTCTTTGATGAACAATTGAAGAGCCATCTGGGCCG17.6923076923076925No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT17.6923076923076925No Hit
GGATCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTT17.6923076923076925No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)