FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R1_G12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R1_G12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGAGATCAAAAGTCCTTAAAAGGAAGAGGCAACACCCTTGGCCTCGAT21.4925373134328357No Hit
GGTTGATTTCTGGTGATATATGGAATCATCGCCAGGAACATTAGAGGATT10.7462686567164178No Hit
CATCAGTATCATTTCTCAAATGAGACCTTCCTTTTATAATGAACCCATAA10.7462686567164178No Hit
AACAAGTTTGCTGCAATATGCACACATTTGGAAGTTTGTTTCATGTATTC10.7462686567164178No Hit
CTATCAACTGGATCAATCTCCTGGTTGCCTTTCTGAGAATAGCTGTTGCT10.7462686567164178No Hit
GTGAAAGGAGTTGGAACAATAGCAATGGAGTTAATCAGAATGATCAAACG10.7462686567164178No Hit
CTATCCAGCTTGAATCAGTCAATTCACATGCCTTATTGAATTCATTTTGG10.7462686567164178No Hit
TTGTATAACATTGTGGGATCTTGTGCCCATTGAATTTTCACAATTTCCCA10.7462686567164178No Hit
GTTTACAGAATCAATCTCTTTATGACTGAAAAAGGTATTCATTGGATTAC10.7462686567164178No Hit
GTCCTGTAGAATCTGTCCACTCCTGCTTGTATTCCCTCATGGTTTGGTGC10.7462686567164178No Hit
ATGTTAGGCTCTTTCCAGCCAAAGAATTTCTTCATGCATTTGATTGGATC10.7462686567164178No Hit
ATTAACCACCTTATTTCCTCAAATTTCTTTCCCAATTGCTCTCGCCACTT10.7462686567164178No Hit
GCTTTGGAGTGTCTGGAGTAAATGAATCAGCTGACATGAGTATTGGAGTA10.7462686567164178No Hit
CATTAATGTAGGATTTGCTGAGCTTTGGGTATGAATTTCCTTTTTTAACT10.7462686567164178No Hit
ATGGATTTGTGGCTAATTTTAGCATGGAGCTACCCAGCTTTGGAGTGTCT10.7462686567164178No Hit
TCCCAATGATATTTGCTGCAATGACGAGAGGATCACTTGAATCGCTGCAT10.7462686567164178No Hit
GAACAACATGATAAACAATGACCTTGGACCTGCAACGGCCCAGATGGCTC10.7462686567164178No Hit
GGGTAGCTCCATGCTAAAATTAGACACAAATCCATAGCGATAAAAAAAGC10.7462686567164178No Hit
AACCCATACAGGTTTGGATTCCGTCGTCCTTCTTTGGTCCTACATTTGCG10.7462686567164178No Hit
AGACAAGACCAATCATGTCACCTCAGACTAACGGAATTTTAGGATTATTC10.7462686567164178No Hit
GAATAAAAGAACTGAGAGATCTAATCTCGCAGTCCCGCACTCGCGAGATA10.7462686567164178No Hit
CTTATTTTCAGTGTGTGGAGGTTGCCCGTTAGCACTTCTTCTTCTTTCTT10.7462686567164178No Hit
CTATGAGAGCAAAATCGTCGGATGATTGGAGCCCATCCCACCAGTATATT10.7462686567164178No Hit
TCTTTATCACTGTTACTCCAATACTCATGTCAGCTGATTCATTTACTCCA10.7462686567164178No Hit
CTATTAGAAGAGGACTTATTTTCTAAATTTTATTCTTTGTTGATTAATTG10.7462686567164178No Hit
CCGCAGGTGCTGCGGGGAAAGGAGTTGGAACAATAGCAATGGAGTTAATC10.7462686567164178No Hit
CACAAGCAGGCAGGCAGGATTTATGTGCAACTGATCCCCTCAGAATGAGT10.7462686567164178No Hit
CCCCGTGGGCTTGTGGGGATAATATAACGATTACTCTTGGGAATCCAGTA10.7462686567164178No Hit
GTGGTGAACAGTATATGTAACACAAAAAGGGTAGAGAAGCCTAATTTTCT10.7462686567164178No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGGCTGGCT10.7462686567164178No Hit
CCAGTAGAATCTGTCCACTCCTGCTTGTATTCCCTCATGGTATGGTGCAT10.7462686567164178No Hit
GGCCCAGATGGCTCTTCAATTGTTCATCAAAGACTACAGATACACATATA10.7462686567164178No Hit
TGACCAAGAAGATGGTCACGCAAAGAACAATAGGGAAGAAAAAACAAAGA10.7462686567164178No Hit
GGCTAATAGATTTCTTAAAGGATGTAATGGAATCAATGAACAAAGACGAA10.7462686567164178No Hit
GCTGCATTGAGGGCAAGCTTTCCCAAATGTCAAAAGAAGTGAACGCCACA10.7462686567164178No Hit
CAATTATTGCCTCAGCAATTGACTGCTCGTCTCTCCCGCTTACTATCAAC10.7462686567164178No Hit
GTCTGGGCTACCAGTAGGGGGCAATGAAAAGAAGGCCAAACTGGCAAATG10.7462686567164178No Hit
GGTGTAGTCCGCTTTGGTGGCCATCTCCTCTCCAGTGAATGAAAAGATGT10.7462686567164178No Hit
CATTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGCTTGC10.7462686567164178No Hit
AGGCAGGATTTATGTGCAACTGATCCCCTCAGAATGAGTGCTGACCGTGC10.7462686567164178No Hit
GTATACGAAACCTCTAATCTGCATCCCAGGTGTTGCGATCGCCCTTCCTG10.7462686567164178No Hit
GGGATGAAGAATTCACAATGGTTGGGAGAAGAGCACCAGCTATTCTCAGA10.7462686567164178No Hit
CTTCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCGTTGCCTTTTCG10.7462686567164178No Hit
CACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGT10.7462686567164178No Hit
CTCCCAACCATTGTGAATTCTTCATACCCTTCATGTACTCTTATTTTCAG10.7462686567164178No Hit
AATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGCCTGTC10.7462686567164178No Hit
CACATTCACAGTCAATGAGGAAAATTGCATCCTACTCTGTTCTGGTCGGG10.7462686567164178No Hit
CTTCTCCTCCTGGAGTGTACATCTGCTCCCAGCACGTCCCTTGGGTTAAG10.7462686567164178No Hit
CATATAGCCTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC10.7462686567164178No Hit
AGGTCTGGTGCCGCAGGTGCTGCGGTGAAAGGAGTTGGAACAATAGCAAT10.7462686567164178No Hit
GTCCTGTAGCTCTGCTAGCACCTGCTTCCAAGCCATGAGGTAATTGGGAT10.7462686567164178No Hit
CCAGTATATTGTCTGGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGC10.7462686567164178No Hit
GGGATCTTTTGACCATTGAATTTTCACAATTTCCCAGTTCCTGATTATCC10.7462686567164178No Hit
GAACACAGATCTTGAGGCTCTCATGGAATGGCTAAAGACAAGACCAATCT10.7462686567164178No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT10.7462686567164178No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC10.7462686567164178No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA10.7462686567164178No Hit
GTTGGAGATATGTCTTGTGAAAATCTTTTTTCCTCCAGTGGGTCAACTTT10.7462686567164178No Hit
ACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACAT10.7462686567164178No Hit
GTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAA10.7462686567164178No Hit
CTCATGGTTTGGTGCATTCACTATGAGAGCAAAATCGTCGGATGATTGGA10.7462686567164178No Hit
GTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCA10.7462686567164178No Hit
AAATCTCCCTGTCTTATTTATATAGGACTTCTTTTTGCTCATGTTGATTC10.7462686567164178No Hit
GAGTGGTAGTGAGTATTGACCGATTTTTAAGGGTTAGAGATCAAAGAGGG10.7462686567164178No Hit
GTCCTGGGATGATGATGGGCATTTTCAACATCCTAATTACGGTCTTGGGA10.7462686567164178No Hit
CATTATCACTGACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGATAA10.7462686567164178No Hit
AAGTATGCTAGAGTCCCGTTTTAGTTTCATTACACACACAACGTCCCCTT10.7462686567164178No Hit
GGAATGAACAAGGACAAACAATCTGGAGCAAAACAAACGATGCTGGATCA10.7462686567164178No Hit
GTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAA10.7462686567164178No Hit
CGCATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTT10.7462686567164178No Hit
GGAAAATTGCATCCTACTCTGTTCTGTTGGGGCAGCAGCAAAGTTTAGAA10.7462686567164178No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTAAACATCAGGA10.7462686567164178No Hit
GTAAGGTCCTGCACACTTTCCCAATAGAGCCTTCCTCCACTCCCCTGGGT10.7462686567164178No Hit
CTTCAGGTCAATGCTTGCTAGCATTTCTGCTGGTATTTGTGTTCGAATCT10.7462686567164178No Hit
GTGTCCTTACGAATCCACTGTACCGGCTTCTGGTTGCCTTAGGGACAAGA10.7462686567164178No Hit
GTCTTGCCATTTTGTTTGAGAACATTATGGGTGCCATGCTCAGGATGTTT10.7462686567164178No Hit
GAATAGAACGATTCCTCTTGGGAATCCAGGAATGTGTAGTTGCAACGGCA10.7462686567164178No Hit
GAACAGGCAGCGGAGGCCATTGAGGTTGCTAATCAGACTAGGCAGATGGT10.7462686567164178No Hit
AGATAGCCTCTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTG10.7462686567164178No Hit
CCATTAGCTCCCATTTTAAGCAGACTTCAGGAATGTGAAGATTCCGTATA10.7462686567164178No Hit
GTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTG10.7462686567164178No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA10.7462686567164178No Hit
CTTTAGAGCGAATGTCCTACCAGCGAATGCGTCAGGAAGGCTAATAGCGG10.7462686567164178No Hit
TCACATGCCTTATTGAATTCATTTTGGACCCAGCTTGCTAGAGATCTGGG10.7462686567164178No Hit
GATTCTACAGGACCTGCAAGTTAGTGGGAATCAACATGAGCAAAAAGAAG10.7462686567164178No Hit
AGCGAGGACTGCAGCGTAGACGCTTTGTCCAAAATGCCCTAAATGGGAAT10.7462686567164178No Hit
TGTACCTACTTCGCGCTACCTTTCTGACATGACCCTCGAGGAAATGTCAC10.7462686567164178No Hit
TTTAGCAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACTTT10.7462686567164178No Hit
TTCATGTACTCTTATTTTCAGTGTTTGGAGGTTGCCCGTTAGCACTTCTT10.7462686567164178No Hit
GAGGCAGAGGGGAGCATGCGGTGGGGAGGCTGTCTCTGCGCCAGGTGGCT10.7462686567164178No Hit
GATAAATACATTTGAAAAAAAGACGATCAGTAATCCACAATATCAGGTGC10.7462686567164178No Hit
CATAATCTCAATATGGATTAGCCACTCAATTCAACTTGGGAATCAAAATC10.7462686567164178No Hit
TTATATACCTTAGGGTAATGAACTGTACTTGTTGTTGGGCCATTCCTATT10.7462686567164178No Hit
GTCCTACATTGTGGAAACACCTAGTTCAGACAATGGAACGTGTTACCCAG10.7462686567164178No Hit
ACCCATTTGAATGGATGTCAATACGCCTCTACTTTTCCTAAAAATTCCCG10.7462686567164178No Hit
ACCTAGAAGAGCATCCCAGTGCTGGGAAGGACCCTAAGAAAACAGGAGGA10.7462686567164178No Hit
ACAATATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTCAT10.7462686567164178No Hit
GATTAGTGGATTGGTGGTAGTAGCCATCTGTCTGTGAGACCGATGCTGTG10.7462686567164178No Hit
GTCCACTCCTGCTTGTATTCCCTCATGGTTTGGTGCATTCACTATGAGAG10.7462686567164178No Hit
GAGTAACAGTGATAAAGAACAACATGATAAACAATGACCTTGGACCTGCA10.7462686567164178No Hit
CAAGAGGATAATGGACATGATTCCAGAGAGGAATGAACAAGGACAAACCC10.7462686567164178No Hit
ACACTAAGTGGAATGAAAATCAAAATCCTCGAATGTTCCTGCCGATGATT10.7462686567164178No Hit
CTATATGTGTATCTGTAGTCTTTGATGAACAATTGAAGAGCCATCTGGGC10.7462686567164178No Hit
CAATCAAGGAATTCTTTGAAAACAAATCCGAAACATCGCCAATCGGAGAG10.7462686567164178No Hit
GCCTTGCATATGTCTACGGCTTGTTCCTCAGTTGGATTCTGTCTAAGGAT10.7462686567164178No Hit
ATTCTCTGTCGCTTTCAATCTGTGCCGCATTTCTTCAATTAACCACCTTA10.7462686567164178No Hit
CAGGCAATCTGAGGGGGCGTGGTGTCGTCCTCAAGAATGGTTCAATTTTG10.7462686567164178No Hit
GGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTCCTTAAA10.7462686567164178No Hit
TGGCCAGGGTTTGTATCAACTCTCCTCCTTATTTTAACTTGAATTCTGAA10.7462686567164178No Hit
TGCTCTCATAGTGAATGCACCAAACCATGAGGGAATACAAGCAGGAGTGG10.7462686567164178No Hit
AGATTCAGCCGAAAACCCCTCAAGTTGTGGAGACGCATATAGACTGTTGA10.7462686567164178No Hit
ACACTAAGTGGAATGAAAATCAAAATCCTCGAATGTTCCTGGCGATGATT10.7462686567164178No Hit
GGGTACATGTTCGAGAGTAAAAGAATGAAGATTCGAACACAAATACCAGC10.7462686567164178No Hit
ACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATT10.7462686567164178No Hit
ACCAAGATGCATTAAGCAAAACCCACGCAACATTAATCAGGCACTCCTCG10.7462686567164178No Hit
CAGATGGAATGCCACACCACCTTAGTCCCTCACAGAATGATTAAACATCG10.7462686567164178No Hit
CTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATGGG10.7462686567164178No Hit
TCTTCAGTCTCTTCAGCAGATTGAGAGCATGATTGAGGCCGAGTCTTCTG10.7462686567164178No Hit
ATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTC10.7462686567164178No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC10.7462686567164178No Hit
GCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAGCACACAC10.7462686567164178No Hit
GTTCTCTTCATGTTCTTTGTCCTTGGGATCTTCTCTTCATTTTCAATGTA10.7462686567164178No Hit
ATCCGCAAGCGATTTGCAGACAATGGATTGGGTGATGCCCCATTCCTTGA10.7462686567164178No Hit
ATGATCGCCTGGTCCAATCGCACGCAAAGAGGGCCTATTATCTTTTGCCT10.7462686567164178No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC10.7462686567164178No Hit
CAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTCTCAATTTTCT10.7462686567164178No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTGATTAGCAAC10.7462686567164178No Hit
CCCTGGCATCAATCCGGGCCCTAGACACCATGGCCTCCACCATGCTAGAA10.7462686567164178No Hit
GGATACACCATGGCCACAGTAAACAGAACACACCAATACTCAGAAAAGGG10.7462686567164178No Hit
GGCTTGGGAGTCTCGGTACTGATGCTTGGCCTAACGAAATAGCCCAAGAC10.7462686567164178No Hit
GAATAAAAGAACAGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA10.7462686567164178No Hit
ACCTATATGTGTATCTGTAGTCTTTGATGAACAATTGAAGAGCCATCTGG10.7462686567164178No Hit
GGTGTATCTGAAATGATAATACCAGATCCAGCATTTCTTTCCATTTCGAA10.7462686567164178No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers