FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R1_G07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R1_G07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2596
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA90.34668721109399075No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC90.34668721109399075No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC80.30816640986132515No Hit
CTTTTCAAACCGTATTTAAAGCGACGATAAATACATTTGAAAAAAAGACG60.23112480739599386No Hit
GGCTAATAGATTTCTTAAAGGATGTAATGGAATCAATGAACAAAGAGGAA60.23112480739599386No Hit
GTCTGGGCTCCCAGTAGGGGGCAATGAAAAGAAGGCCAAACTGGCAAATG60.23112480739599386No Hit
ATGTTATACAACAAAATGGAATTTGAACCATTTCAGTCTCTTGTCCCTAA60.23112480739599386No Hit
GTCTGGAGTAAATGAATCAGCTGACATGAGTATTGGAGTAACAGTGATAA60.23112480739599386No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG50.1926040061633282No Hit
GTCTTATTTATATAGGACTTCTTTTTGCTCATGTTGATTCCCACTAACTT50.1926040061633282No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG50.1926040061633282No Hit
TATGTATTCAGTAGCCCTGCAGTGGGACACTTCTGCTGTAAAATAGTTCC50.1926040061633282No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG50.1926040061633282No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT50.1926040061633282No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA50.1926040061633282No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG50.1926040061633282No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG50.1926040061633282No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA40.15408320493066258No Hit
CTTGTGAACAGATTGCTGATTCACAGCATCGGTCTCACAGACAGATGGCT40.15408320493066258No Hit
ACACTAAGTGGAATGAAAATCAAAATCCTCGAATGTTCCTGGCGATGATT40.15408320493066258No Hit
CCCCTGGGTGACTCTCCGATTGGCCATGTTTCCGATTTGTTTTCAAAGAA40.15408320493066258No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG40.15408320493066258No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA40.15408320493066258No Hit
CATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTT40.15408320493066258No Hit
CTCCAAAGCTGGGTAGCTCCATGCTAAAATTAGCCACAAATCCATAGCGA40.15408320493066258No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT40.15408320493066258No Hit
GTCCTGGGATGATGATGGGCATGTTCAACATGCTAAGTACGGTCTTGGGA40.15408320493066258No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA40.15408320493066258No Hit
GTCTCTTACTCTCCTTTTTCTTTGAAAGTGGGTTGTTATCTCTATTTCCT40.15408320493066258No Hit
GTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTCCCAAGAC40.15408320493066258No Hit
CTGCAGCGTAGACGCTTTGTCCAAAATGCCCTAAATGGGAATGGGGACCC40.15408320493066258No Hit
CTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCA40.15408320493066258No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC40.15408320493066258No Hit
ACTCCAGACACTCCAAAGCTGGGTAGCTCCATGCTAAAATTAGCCACAAA40.15408320493066258No Hit
CATTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTC40.15408320493066258No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG40.15408320493066258No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA40.15408320493066258No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG40.15408320493066258No Hit
GTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCAC40.15408320493066258No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC40.15408320493066258No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC40.15408320493066258No Hit
CAATAAGGCATGTGAATTGACTGATTCAAGCTGGATAGAACTTGATGAAA40.15408320493066258No Hit
TCCACCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCAT40.15408320493066258No Hit
CATCAGGCTGACCACTTGGCTGTTTTGGAGTAATTTGAATGGGTCTATCC30.11556240369799693No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT30.11556240369799693No Hit
CTTTAGATCGAATGGCCTAACAGCTAATGAGTCAGGAAGGCTAATAGATT30.11556240369799693No Hit
AAGTACTTCAGGTCAATGCTTGCTAGCATTTCTGCTGGTATTTGTGTTCG30.11556240369799693No Hit
TTCAAAAGGGAGATTCCGCTGCACTGAGAATGTAGGCTGCACACTGATCT30.11556240369799693No Hit
GTTCATTACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTT30.11556240369799693No Hit
ATTACAAAGAGAACCGGTTCATTGAAATTGGAGTAACACGGAGGGAAGTC30.11556240369799693No Hit
ACTTAGTGTTGTCCCCAGTGATTGTGAAAGAAATCTCTGTGTCTTGTGAA30.11556240369799693No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG30.11556240369799693No Hit
GATTGGAGGAGTAAGGATGGTGGACATCCTTAGACAGAATCCAACTGAGG30.11556240369799693No Hit
GTCTTGGGAGTCTCGATACTGAATCTTGGACAAAAGAAATACACCAAGAC30.11556240369799693No Hit
GACGAGAGGATCACTTGAATCGCTGCATCTGCACTCCCATTCGCTTCTGG30.11556240369799693No Hit
AAGAAGTCCTATATAAATAAGACAGGGACATTTGAATTCACAAGCTTTTT30.11556240369799693No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT30.11556240369799693No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG30.11556240369799693No Hit
ATCCTACTCTGTTCTGGTGGGGCAGCAGCAAAGGGGAGAAGTTTTATTAT30.11556240369799693No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC30.11556240369799693No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG30.11556240369799693No Hit
AGTCAACACTTATCAATGGATAATCAGGAACTGGGAAATTGTGAAAATTC30.11556240369799693No Hit
GAATCAATCTCTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGTCT30.11556240369799693No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA30.11556240369799693No Hit
CCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCA30.11556240369799693No Hit
CTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGATAA30.11556240369799693No Hit
GCCGAAAACCCCTCAAGTTGTGGAGACGCATATAGACTGTTGAATACAGA30.11556240369799693No Hit
GTAAAATAGTTCCTCCTCATGCTTGCGATATGTTCAATCGGGGCAACATC30.11556240369799693No Hit
CTCTTATTTTCAGTGTTTGGAGGTTGCCCGTTAGCACTTCTTCTTCTTTC30.11556240369799693No Hit
CTATAAGACAAGAAATGGCCAGTAGGAGTCTATGGGATTCCTTTCGTCAG30.11556240369799693No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA30.11556240369799693No Hit
GTGCCAAGGAGGCACAGGATGTGATTATGGAAGTTGTTTTCCCAAATGAA30.11556240369799693No Hit
GTGCTGGCTAGCACTACGGCAAAGGCTATGGAACAGATGGCTGGATCGAG30.11556240369799693No Hit
GTACATAAATACGGCCTTGCTCAATGCATCCTGTGCAGCCATGGATGACT30.11556240369799693No Hit
CTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTT30.11556240369799693No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTGATTAGCAAC30.11556240369799693No Hit
CTACCTGAGGATAATGAACCAAGTGGGTATGCACAAACAGACTGTGTTCT30.11556240369799693No Hit
TCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCTAAG30.11556240369799693No Hit
TCTAATAAGAGCACTGACATTAAATACGATGACCAAAGATGCAGAGAGAG30.11556240369799693No Hit
GAATACCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGG30.11556240369799693No Hit
CTGTTACTCCAATACTCATGTCAGCTGATTCATTTACTCCAGACACTCCA30.11556240369799693No Hit
CATTAAATACGATGACCAAAGATGCAGAGAGAGGCAAGTTAAAAAGAAGG30.11556240369799693No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT30.11556240369799693No Hit
CTACAGGACATTGAAAATGAAGAGAAGATCCCAAGGACAAAGAACATGAA30.11556240369799693No Hit
ATAGTACGTTCCCTCTTTGATCTCTAACCCTTAAAAATCGGTCAATACTC30.11556240369799693No Hit
ATCTCCTGGTTGCCTTTCTGAGAATAGCTGTTGCTCTTCTCCCAACCATT30.11556240369799693No Hit
GTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTTGA30.11556240369799693No Hit
CCTGTGAGAATACCATGGCCACAATTATTGCCTCAGCAATTGACTGCTCG30.11556240369799693No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG30.11556240369799693No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT30.11556240369799693No Hit
GCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCCA30.11556240369799693No Hit
GTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCA30.11556240369799693No Hit
TTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGCGGAAAAGGCA30.11556240369799693No Hit
TCTCAGGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAG30.11556240369799693No Hit
AAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCTTTC30.11556240369799693No Hit
GAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGG30.11556240369799693No Hit
CTACAGGACCTGCAAGTTAGTGGGAATCAACATGAGCAAAAAGAAGTCCT30.11556240369799693No Hit
GATTAGAGGTTTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCG30.11556240369799693No Hit
ATACTGATGTGGTGAACTTTGTAAGTATGGAGTTCTCACTCACTGACCCG30.11556240369799693No Hit
GTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAA30.11556240369799693No Hit
ACATGATAAACAATGACCTTGGACCTGCAACGGCCCAGATGGCTCTTCAA30.11556240369799693No Hit
GTATTGGAGTAACAGTGATAAAGAACAACATGATAAACAATGACCTTGGA30.11556240369799693No Hit
TTCTTTGGCTGGAAAGAGCCTAACATAGTCAAACCACATGAGAAAGGCAT30.11556240369799693No Hit
GTCATACTGTTTAAGGTCTCCAACATCTTTGCAGTCATCAAAGTCTACTT30.11556240369799693No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT30.11556240369799693No Hit
CCCTTAGTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTC30.11556240369799693No Hit
ACCTAGAGAAAGCCAACAAAATAAAATCTGAGAAGACACACATTCACATC30.11556240369799693No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT30.11556240369799693No Hit
ATCCTGACCCCTGCTCATTTTGATGGTGATAACCGTACCATCCATCTACC30.11556240369799693No Hit
CCTTAAACAGTATGACAGTGATGAGCCAGAGCCCAGATCTCTAGCAAGCT30.11556240369799693No Hit
GTTCCAGGTTGTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGAT30.11556240369799693No Hit
GGAGAGGAGATGGCCACCAAAGCGGACTACACCCTTGACGAAGAGAGCAG30.11556240369799693No Hit
GTATAACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGT30.11556240369799693No Hit
TCACATGCCTTATTGAATTCATTTTGGACCCAGCTTGCTAGAGATCTGGG30.11556240369799693No Hit
GGCAAATGTTGTGAGAAAGATGATGACTAATTCACAAGACACAGAGATTT30.11556240369799693No Hit
GTCTATGGGATTCCTTTCGTCAGTCCGAAAGAGGCGAAGAGACAATTGAA30.11556240369799693No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT30.11556240369799693No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA30.11556240369799693No Hit
GCGGAAAAGGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAA30.11556240369799693No Hit
CCTCATTTCCATGCCCCATTTCATCTTGATCTTGGAGGTTCCATTGGTTC30.11556240369799693No Hit
TTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAG30.11556240369799693No Hit
ATGCAGAGAGAGGCAAGTTAAAAAGAAGGGCTATCGCAACACCTGGGATG30.11556240369799693No Hit
CTTCAATTGTTCATCAAAGACTACAGATACACATATAGGTGCCATAGGGG30.11556240369799693No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers