FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R1_G01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R1_G01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTCAGGTAGACTGTTTACTTTGTAATATCCGCAAGAGAGTTGCAGACA14.545454545454546No Hit
GCAACAGAGCTGCGAGTCCGGGAGTCCCAGTCAGCCAGGTTTTATAGGAC14.545454545454546No Hit
GATAATAGGCCCTCTTTGCGTGCGATTGGACCAGGCGATCATGGAAAAGA14.545454545454546No Hit
AAATACCAATCAACAAAAGGCATCCGCAGGCCAGATCAGTGTGCAGCCTA14.545454545454546No Hit
CTTTCAGTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAA14.545454545454546No Hit
ATCGAGAAGGGAGAATGAACTATTACTGGACACTAGTAGAGCCGGGAGAC14.545454545454546No Hit
GTCAGACTCATATGCCTTGACTCCGAATAAAAAATCCCCAACCTAACGAA14.545454545454546No Hit
CTCTTCCATACTGTTGGTAGCGGTTACCATTGCTACCGATGCTCCCAAGG14.545454545454546No Hit
ATTCAAATGAAAAGAACTTATAAGAAAAGGTAATAATCCATCTAAAAAAA14.545454545454546No Hit
GCTTTAGTCTTTACCATGGGAGCTCAATCGGACAGGATTAGCTCAACATT14.545454545454546No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG14.545454545454546No Hit
ATTCCCCAGTGGTCCATTGCTTCATCAAGGCCCAGCACTGGTATTACTAC14.545454545454546No Hit
AATATGAACTGCTAGCGAAAAATTTCTAGAATACATAGCCGCCCTTCAGG14.545454545454546No Hit
TCCATAGCCTTTGCCGTAGTGCTAGCCAGCAACATTCTGTTTTCATGCCT14.545454545454546No Hit
ACACTAAGTGGAATGAAAATCAAAATCCTCGAATGTTCCTGGCGATGATT14.545454545454546No Hit
CACCAATTGACTCTTGTGAGCTGGGTTTTAATTTTGTCTCATCAGGATGC14.545454545454546No Hit
AGTTTTTTACTCTAGCTCTATGTTGACAAAATGACATTCGTCAACATCCA14.545454545454546No Hit
CTTTCAAAATATATAATTCCTCCAATCCCCTTGTGGCCAATCCTATTTCA14.545454545454546No Hit
TGTTACATGTATTCGGATTTCCATTTCATCGACGAACGGGGTGGATCAAT14.545454545454546No Hit
CTGTGTTGTGTGAATTAGTCCTCATCTTTATCACAACATTTGCCAGTTTG14.545454545454546No Hit
CTTGTTGGGAAAAAAATCGTGTAATGGATATTTAAAGATGAATACAGCGA14.545454545454546No Hit
GGTCATATACTGTTCACCACGGTCCAGGCCATGATTCGGTCTCTTCCTTC14.545454545454546No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)