FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R1_F09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R1_F09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG11.0309278350515463No Hit
CATATGAACAAATGGAGACTGGTGGGGAGCGCCAGGATGCCACAGAAATC11.0309278350515463No Hit
GTCCTTACGAATCCACTGTACCGGCTTCTGGTTGCCTTAGGGACAAGAGA11.0309278350515463No Hit
GGCTAATAGATTTCTTAAAGGATGTAATGGAATCAATGACCAAAGCGGAA11.0309278350515463No Hit
CTCCTGGAGTGTACATCTGCTCCCAGCACGTCCCTTGGGTTAAGTGCAAC11.0309278350515463No Hit
ACCATGGCCTCCCCACCCAGTACACACGAACCAGCCATCGGTACCATAGC11.0309278350515463No Hit
GCAAATAGTAGCATTGCCACAACTACTTCAGTGGATGTGTGAGGAAGGAG11.0309278350515463No Hit
GTACCCTTCCCTTTCAAAGTCATGCCCACTTGCTACTGCAAGCCCATACA11.0309278350515463No Hit
ATATACATTTGCACCCGCAAATGCAGACACATTATGTATAGGTTATCATG11.0309278350515463No Hit
TTCCACAAGAGGGGTCCAGATTGCTTCAAATGAGAATGTGGAAACCATGG11.0309278350515463No Hit
GTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATTTCAGA11.0309278350515463No Hit
GAACATGAAGAGAACAAGCCAATTGAAGTGGGCACTCGGTGAAAATATGG11.0309278350515463No Hit
GTTTCTAGCAGCGATAATCAAACTTTGGTCAACATCATCATTTCTCACTT11.0309278350515463No Hit
AAGTACAACGGCATAATAACAGACACTATCAAGAGTTGGAGAAACAATAT11.0309278350515463No Hit
GAAATAACGTTCCATTGGGCCAAGGAGGTGTCACTAAGCTATTCAACTGG11.0309278350515463No Hit
ATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATGGGAGCTAATG11.0309278350515463No Hit
CAATTGCTGAGGCAATAATTGTGGCCATGGTATTCTCACAGGAGGATTGC11.0309278350515463No Hit
CTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATGCAAACTAAGA11.0309278350515463No Hit
GAACTTTGTCAATAGGGCAACCCATCGACTGAACCCCATGCACCAACTCT11.0309278350515463No Hit
CCTCAGCAATTGACTGCTCGTCTCTCCCGCTTACTATCAACTGGATCAAT11.0309278350515463No Hit
GTAGATGGATGGTGAATGCCCCATAGCACGACGACTTCTGTCCCGTAATC11.0309278350515463No Hit
CCCTGGAAGGACAAATCTTCTGGCTTTGCACTTTCCATCATTCTTATAAC11.0309278350515463No Hit
CCCAGGGATCATTAATCAGGCACTACTCGATTGCTTCATATAGCCCCCCA11.0309278350515463No Hit
TTTATGTACACTCCATTCATTATGTATTCAGTAGCCCTGCAGTGGGACAC11.0309278350515463No Hit
CTAAGACCACTGTGGACCATATGTCCATAATCAAAAAGTACAAATCAGGA11.0309278350515463No Hit
GCAGAGTGGCATGCCATCCACACCAATTGCCGCTTGTGAGCTGGGTTTTC11.0309278350515463No Hit
GTGCCAAGGAGGCACAGGATGTGATTATGGAAGTTGTTTTCCCAACAAAA11.0309278350515463No Hit
GTGCAGCCATGGATGACTTTCAGCTGATCCCAATGATAAGCAAATGTAGG11.0309278350515463No Hit
GTCTACGGCTTGTTCCTCAGTTGGATTCTGTCTAAGGATGTCCACCATCC11.0309278350515463No Hit
CCTCAACCCTATTGCTGCCTTGCATATGTCTACCTCTTGATCCTCAGTGG11.0309278350515463No Hit
TATCCATTTGAATGTTTGTCATTTAGAAAGGCCCATTTCGTCAAGAACAA11.0309278350515463No Hit
TTTTGTAGAACAACTTCCATTGTTTCAATGCATGAATTATCAAATATTCC11.0309278350515463No Hit
GCACAGGATGTTTCTGAACCACTCGGCTTGATTTATTGTGATATATGTAA11.0309278350515463No Hit
CTGGAGAATTGCCTCTTACCAGTATCCTCAACCCTGATCCTCTCACATTC11.0309278350515463No Hit
GCGGAAAAGGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAA11.0309278350515463No Hit
TCGTCCGAGAGCTCGAAGACTCCCCGCCCCTGGAAGGACAAATCTTCTGG11.0309278350515463No Hit
GAGCAGCAGTGTCAGCAGCCCCATTAGCATCTCTCTTGGAAATGTGCCAC11.0309278350515463No Hit
AGAAGGTACATGCAATGAGAACTATTGGGACTCATCCTACCTCCAGATAT11.0309278350515463No Hit
TCTCCCAACCATTGTGAATTCTTCATACCCTTCATGTACTCTTATTTTCA11.0309278350515463No Hit
CCATCTGCCTAGAATGATTAGCAACCTAAATGCACTCCGCAGCCTGTTCA11.0309278350515463No Hit
GCTGACACTGCTGCTCTTCTTACTATGTTTCTAGCAGCGATAATCAAACT11.0309278350515463No Hit
CACCCATAATGTTCTCAAACAAAATGGCAAGACTAGGGATAGGGTACATG11.0309278350515463No Hit
GGTTGAGAACATTATGGGTGCCATGCTCAGGATGTTTCTGACCCACTCGG11.0309278350515463No Hit
GTGTTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT11.0309278350515463No Hit
ATGTTATACAACAAAATGGAATTTGAACCATTTCAGTCTCTTGTCCCTAA11.0309278350515463No Hit
GTGTGCTCTTCAGGTCGGCTGCATATCCTGACCCCTGCTCATTTTGATGG11.0309278350515463No Hit
GTCAACATCCACAGCACTCTCCTGTTCCTCTTTATATTCTACCCTCATGG11.0309278350515463No Hit
GTAACACGGAGGGAAGTCCACATATATTACCTAGAGAAAGCCAACAAAAT11.0309278350515463No Hit
TTCTGGTGCCATATTTTCACCGAGTGCCCCCTTCAATTGGCTTGTTCTCT11.0309278350515463No Hit
GGGCAACATCTTCTCCTATTTCATCAAGTTCTATCCAGCTTGAATCAGTC11.0309278350515463No Hit
GATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGA11.0309278350515463No Hit
GAATCATGGCCTGGACAGTGGTGAACAGTATATGTAACACAACAGGGGTA11.0309278350515463No Hit
CTTCGGGAGACAATAGTACGTTCCCTCTTTGATCTCTAACCCTTAAAAAT11.0309278350515463No Hit
CAAAGAGGAAATAGAGATAACAACCCACTTTCAAAGAAAAAGGAGAGTAA11.0309278350515463No Hit
ATGGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAG11.0309278350515463No Hit
ATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCACATTTCCAA11.0309278350515463No Hit
GTTCAATTTTGGCGTTCACTTCTCTTGACATTTGGGAAAGCTTGCCCTCA11.0309278350515463No Hit
CTATTGTTCTTTGCGTGACCATCTTCTTGGTCATGTTGTCTCTTACTCTC11.0309278350515463No Hit
TGGAGGACCAAACTTATACAATATACGGAATATTCACATTCCTGAAGTAT11.0309278350515463No Hit
AAATAAGAGTACATGAAGGGTAGGAAGAATTCACAATGGTTGGGAGAAGA11.0309278350515463No Hit
ATCAAGAGTTGGAGAAACAATATATTGATCACACAAGAGTCTGAATGTGC11.0309278350515463No Hit
GAGAAAAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGATACCC11.0309278350515463No Hit
CTCATAGTGAATGCACCAAACCATGAGGGAATACAAGCAGGAGTGGACAG11.0309278350515463No Hit
CCTATATGTGTATCTGTAGTCTTTGATGAACAATTGAAGAGCCATATGGG11.0309278350515463No Hit
ATACAGTAAAGACAACAGTGTAAGAATCGGTTCCAAGGGGGATGTGTTTG11.0309278350515463No Hit
ACTGAATACATAAAGAAGGGAGTGTACATAAATACGGCATAGCTAAATGC11.0309278350515463No Hit
CAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGGGCC11.0309278350515463No Hit
AAACTAGGCTTTTCACTATAAGACAAGAAATGGCCAGTAGGAGTCTATGG11.0309278350515463No Hit
ACGTAGGAATCACTGTAGATTTCAAAGGACTGTCACGTTCTGTGTTGTAA11.0309278350515463No Hit
GACCCCAAGCACGGAGATGTCGCTGAGAGGGATAAGAGTCAGCAAAATGG11.0309278350515463No Hit
CCCTTGTCCTTCGTCCATTTTCACCCCTCCAGAAATATCGGTCAATGATT11.0309278350515463No Hit
GTGCAGCCATGGATGACTTTCAGCTGATCCCAATGATAAGAAAATGTAGG11.0309278350515463No Hit
CTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTATGGCTTGTGTTGA11.0309278350515463No Hit
TACATATTCTACACTGTAGAGACCCAGTAGAGCACATCCAGAAACTGATT11.0309278350515463No Hit
GTGCTGGAGTATTCATCTACTCCCATTTTGCTGCCTCTTATCCCTCTCAG11.0309278350515463No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT11.0309278350515463No Hit
GGGCCCGAGACACCATGGCCTCCACCATGCTTGAAATTCCCCCAGGTCTC11.0309278350515463No Hit
GGGTTATTCAGTCTTTGTTTTTACTGCGCTAGTGGTCTTTTCGTGCGGGT11.0309278350515463No Hit
CTCCAGTGCTGGTCTGAAAGATGACCTTCTTGAAAATTTGCAGGCCTACC11.0309278350515463No Hit
GTGTTGGCCAATGCAGTTGCTGCCGGTTGATTTCTGTTTAATGTCCAATC11.0309278350515463No Hit
GAGGATCACTTGAATCGCTGCATCTGCACTCCCATTCGCTTCTGGTAGGC11.0309278350515463No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT11.0309278350515463No Hit
TTCCTTGGTCATGTCTTTCTCTTTGACAGAAGACTCGGCCTCAATCATGC11.0309278350515463No Hit
GGCCGAACAGCTAAGGAGTCAGGAAGGCTAATAGATTTCTTAAAGGATGT11.0309278350515463No Hit
CAGCAGAAGTGTCCCAATGCAGGGCTACTGAATACATAATGAAGGGAGTG11.0309278350515463No Hit
GAAAATGGAGAGTAAGAGACAACATGACCAAGAAGATGGTCACGCAAAGA11.0309278350515463No Hit
CCTCCAAACACTGAAAATAAGAGTACATGAAGGGTATGAAGAATTCACAA11.0309278350515463No Hit
GCATCCATTCCAACAACTGTAAGTCCTTAGTTTGCCATGTTGAAATTGAA11.0309278350515463No Hit
GGGGTGACTCAGGGCCATTGATCTCCCACATCATTGATGACGAATAAGTT11.0309278350515463No Hit
CATCTTCTCCTATTTCATCAAGTTCTATCCAGCTTGAATCAGTCAATTCA11.0309278350515463No Hit
GGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTTGAGTTA11.0309278350515463No Hit
GTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATAATCCCGTCAGGC11.0309278350515463No Hit
GCACTAACATTAAATACGATGACCAAAGAAGCAGAGAGAGGCAAGTTAAA11.0309278350515463No Hit
AACTTGCAGGTCCTGTAGAATCTGTCCACTCCTGCTTGTATTCCCTCATG11.0309278350515463No Hit
GTATTCTTGCCCCCACTTCATTTGGGAAAACAACTTCCATAATCACATCC11.0309278350515463No Hit
GAGAAGAACTTATATGAAAAGGTAAGAAGCCAGCTAAAAAACAATGCCAA11.0309278350515463No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC11.0309278350515463No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAATA50.0145.02
AATAGAT50.0145.05
CTAATAG50.0145.03
ATAGATT50.0145.06
GATTTCT50.0145.09
TAATAGA50.0145.04
GGCTAAT50.0145.01
AAAGCCT50.0145.0145
AGATTTC50.0145.08
TAGATTT50.0145.07
Produced by FastQC (version 0.11.5)