FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R1_F07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R1_F07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2347
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG170.7243289305496379No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC130.5538985939497231No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA110.4686834256497656No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA90.3834682573498083No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA90.3834682573498083No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT80.34086067319982954No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG80.34086067319982954No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG80.34086067319982954No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG80.34086067319982954No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT80.34086067319982954No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC80.34086067319982954No Hit
GCGGAAAAGGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAA80.34086067319982954No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA70.2982530890498509No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG70.2982530890498509No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT60.2556455048998722No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG60.2556455048998722No Hit
CTGTCATACTGTTTAAGGTCTCCAACATCTTTGCAGTCATCAAAGTCTAC60.2556455048998722No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT50.21303792074989347No Hit
ACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTCATATAGCCCCCC50.21303792074989347No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG50.21303792074989347No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC50.21303792074989347No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT50.21303792074989347No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTA50.21303792074989347No Hit
ATGCACACATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCG50.21303792074989347No Hit
ATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGC50.21303792074989347No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG50.21303792074989347No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT50.21303792074989347No Hit
GTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTG50.21303792074989347No Hit
AGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGAT40.17043033659991477No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG40.17043033659991477No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG40.17043033659991477No Hit
ACATAATGGACTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTT40.17043033659991477No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC40.17043033659991477No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC40.17043033659991477No Hit
GTATTTTTCCCATTTGTGTGGCTCCAGTCTCGGGTCAGTGAGTGAGAACT40.17043033659991477No Hit
CACATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCAT40.17043033659991477No Hit
ATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATGCA40.17043033659991477No Hit
CATTAAATACGATGACCAAAGATGCAGAGAGAGGCAAGTTAAAAAGAAGG40.17043033659991477No Hit
GGATGTGATTATGGAAGTTGTTTTCCCAAATGAAGTGGGGGCAAGAATAC40.17043033659991477No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC40.17043033659991477No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC40.17043033659991477No Hit
TCTTCATTCTTTTACTCTCGAACATGTACCCTTTCCCTAGTCTTGCCATT40.17043033659991477No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT40.17043033659991477No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA40.17043033659991477No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG40.17043033659991477No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG40.17043033659991477No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA40.17043033659991477No Hit
GTTCCAGGTTGTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGAT40.17043033659991477No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA40.17043033659991477No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCTACAACTACTTCAG30.1278227524499361No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG30.1278227524499361No Hit
GTCTGTGAGACCGATGCTGTGAATCAGCAATCTGTTCACAAGTGGCACAC30.1278227524499361No Hit
CAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTG30.1278227524499361No Hit
ACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACAT30.1278227524499361No Hit
GTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCC30.1278227524499361No Hit
TTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATG30.1278227524499361No Hit
AACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAG30.1278227524499361No Hit
GTATCAGATGGAGGACCAAACTTATACAATATACGGAATCTTCACATTCC30.1278227524499361No Hit
AAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATC30.1278227524499361No Hit
GCTTTGGAGTGTCTGGAGTAAATGAATCAGCTGACATGAGTATTGGAGTA30.1278227524499361No Hit
ATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAGGGAAAAATTT30.1278227524499361No Hit
CTTCAATACTTAATTTCAGAGCATCCATCAGCAGGAACTTTGACCGCTGA30.1278227524499361No Hit
AGTTAGTGGGAATCAACATGAGCAAAAAGAAGTCCTATATAAATAAGACA30.1278227524499361No Hit
GTATTTATGTACACTCCCTTCATTATGTATTCAGTAGCCCTGCAGTGGGA30.1278227524499361No Hit
ACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGATACCCAATTACA30.1278227524499361No Hit
GAATTCATGCCTTGAAACAATGGAAGTTGTTCAACAAACAAGGGTAGATA30.1278227524499361No Hit
CTTGTGAACAGATTGCTGATTCACAGCATCGGTCTCACAGACAGATGGCT30.1278227524499361No Hit
GAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGATACCCAA30.1278227524499361No Hit
TCATTAATCAGGCACTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAA30.1278227524499361No Hit
GTTTTATTATTTGGACAGTGTCAAATGTCCCAAGCACATCCCGCATTTGC30.1278227524499361No Hit
GTCTTATTTATATAGGACTTCTTTTTGCTCATGTTGATTCCCACTAACTT30.1278227524499361No Hit
GTCCAAAATGCCCTAAATGGGAATGGGGACCCGAACAACATGGATAGAGC30.1278227524499361No Hit
CTTCATGTTCTTTGTCCTTGGGATCTTCTCTTCATTTTCAATGTCCTGTA30.1278227524499361No Hit
GTAAAATAGTTCCTCCTCATGCTTGCGATATGTTCAATCGGGGCAACATC30.1278227524499361No Hit
CTTCAGGTCAATGCTTGCTAGCATTTCTGCTGGTATTTGTGTTCGAATCT30.1278227524499361No Hit
GAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAA30.1278227524499361No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC30.1278227524499361No Hit
CATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT30.1278227524499361No Hit
ACATAGGCTCTAAAGTTTTCAAGGCTGGGGAAGTTCGGTGGGAGACTTTG30.1278227524499361No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTGATTAGCAAC30.1278227524499361No Hit
AGACTGGTTCATGCTCATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCG30.1278227524499361No Hit
GCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAG30.1278227524499361No Hit
CTACCTGAGGATAATGAACCAAGTGGGTATGCACAAACAGACTGTGTTCT30.1278227524499361No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT30.1278227524499361No Hit
GAGTAAATGAATCAGCTGACATGAGTATTGGAGTAACAGTGATAAAGAAC30.1278227524499361No Hit
GTCTTACTCATATCATGATTTGGCATTCCAACCTGAATGATGCCACATAT30.1278227524499361No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC30.1278227524499361No Hit
ATGTTGAACATGCCCATCATCATCCCAGGACTCAGTGATGCTGTGCCATC30.1278227524499361No Hit
CAATATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTCATA30.1278227524499361No Hit
CATTAGCATCTCTCTTGGAAATGTGCCACAGCACACAGATTGGAGGAGTA30.1278227524499361No Hit
GTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAA30.1278227524499361No Hit
ATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCA30.1278227524499361No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG30.1278227524499361No Hit
CACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACTGGTGC30.1278227524499361No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT30.1278227524499361No Hit
GTATGGAGTTCTCACTCACTGACCCGAGACTGGAGCCACACAAATGGGAA30.1278227524499361No Hit
TCCAAGAACTGTAAGTCGTTTGGTTGCCTTGTTGTAATTGAATACTGGAG30.1278227524499361No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA30.1278227524499361No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC30.1278227524499361No Hit
GCATCACTGAGTCCTGGGATGATGATGGGCATGTTCAACATGCTAAGTAC30.1278227524499361No Hit
CTCAATGAGTGACATCGAAGCCATGGCGTCTCAAGGCACCAAACGATCAT30.1278227524499361No Hit
ACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAA30.1278227524499361No Hit
GACTGGTGCACCCCAGCTCAACCCGATTGATGGACCACTACCTGAGGATA30.1278227524499361No Hit
CAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGG30.1278227524499361No Hit
CCTATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATGTT30.1278227524499361No Hit
CAATAGAGCCTTCCTCCACTCCCCTGGGTGACTCTCCGATTGGCCATGTT30.1278227524499361No Hit
ATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATA30.1278227524499361No Hit
CCACTACCTGAGGATAATGAACCAAGTGGGTATGCACAAACAGACTGTGT30.1278227524499361No Hit
CATAATGAAGGGAGTGTACATAAATACGGCCTTGCTCAATGCATCCTGTG30.1278227524499361No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG30.1278227524499361No Hit
CTCCAGCACGGAGAGAGTGGTAGTGAGTATTGACCGATTTTTAAGGGTTA30.1278227524499361No Hit
GACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTATTTAAA30.1278227524499361No Hit
TCGGAATACTGCCCGACATGACCCCAAGCACGGAGATGTCGCTGAGAGGG30.1278227524499361No Hit
CTCATTAGCTGTTAGGCCATTCGATCTAAAGACTTCTATGGTGTTGGCCA30.1278227524499361No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCAGG100.00664057145.02
CTCAGGC100.00664057145.03
Produced by FastQC (version 0.11.5)