FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD_S3_R1_F04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R1_F04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATGTGATTATGGAAGTTGTTTTCCCAAATGAAGTGGGGGCAAGAATAC13.7037037037037033No Hit
GTCCTGCACACTTTCCCAATAGAGCCTTCCTCCACTCCCCTGGGTGACTC13.7037037037037033No Hit
ATCGCATTTAATGTCAGTGCTCTTATTAGATAGCCTCTCTTATTCAGTCT13.7037037037037033No Hit
GTTCTTTGCGTGACCATCTTCTTGGTCATGTTGTCTCTTACTCTCCTTTT13.7037037037037033No Hit
CAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAA13.7037037037037033No Hit
GTCCTGTAGAATCTGTCCACTCCTGCTTGTATTCCCTCATGGTTTGGTGC13.7037037037037033No Hit
CAATAAGGCATGTGAATTGACTGATTCAAGCTGGATAGAACTTGATGAAA13.7037037037037033No Hit
TATATGTAATCATCGCCAGGAACATTCGAGGATTTTGATTTTCATTCCAC13.7037037037037033No Hit
ACTAACAAGTTTGCTGCAATATGCACACATTTGGAAGTTTGTTTCATGTA13.7037037037037033No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA13.7037037037037033No Hit
GTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTACTGAGTTG13.7037037037037033No Hit
ATCCAAAATGGAAGACTTTGTGCGACAATGCTACTATCCAATGATCGTCG13.7037037037037033No Hit
GAGGAAAGCTTTCCACAAGAGGGGTCCAGATTGATTCAAATGAGAATGTG13.7037037037037033No Hit
GGAGTGGACAGATTCTACAGGACCTGCAAGTTAGTGGGAATCAACATGAG13.7037037037037033No Hit
TCCTACATCTGTTCCTGCATGCTGTAGTAGGTGGTGTCCGCACATCTGGC13.7037037037037033No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGGTTCATTACC13.7037037037037033No Hit
AACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGAATTAAG13.7037037037037033No Hit
CTGTAGAATCTGTCCACTCCTGCTTGTATTCCCTCATGGTTTGGTGCATT13.7037037037037033No Hit
CACATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCAT13.7037037037037033No Hit
CTTCATCACCAATCAGGACATCAGCCGTGCCACCCATGGCAGCTGGTGGC13.7037037037037033No Hit
CCCCAGCTCAACCCGATTGATGGACCACTACCTGAGGATAATGAACCAAG13.7037037037037033No Hit
CCTATATGTGTATCTGTAGTCTTTGATGAACAATTGAAGAGCCATCTGGG13.7037037037037033No Hit
AGGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTTGAGAATTCA13.7037037037037033No Hit
GAGAATGAACACATTCACAGAGATAGAACCACATTTGCCAAGAGATCACA13.7037037037037033No Hit
GTTATTGGGAGAACTCATGGATTACCTGAAGTTTCAGTATTTACAAAATA13.7037037037037033No Hit
GGCTTGTGTTGAGAATAGAACGATTCCTCTTGGGAATCCAGGAATGTGTA13.7037037037037033No Hit
GGTATGCACAAACAGACTGTGTTCTAGAGGCTATGGCTTTCCTTGAAGAA13.7037037037037033No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)