FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R1_F01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R1_F01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAAGATAAGAGTGCATGAGGGATATGAAGAGTTCACAATGGTTGGGAG12.857142857142857No Hit
ATAAGAAACCGATCATGAGACAATAACACTGATAAATGCTTCAATAATAT12.857142857142857No Hit
GGGGGTCTCAGTGCCAAGGAGGCACAGTATGTAATCATGGAAGTTGGTTT12.857142857142857No Hit
AGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAATGCTG12.857142857142857No Hit
GGCAAGGAGACGTGGTGTTGGTAATGAAACGGAAACGGGACTCTATCATA12.857142857142857No Hit
GAATAAAAGAACTAAGAAATCTAATGTCCCACTCTCGCACCCGCGAGATA12.857142857142857No Hit
CTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGG12.857142857142857No Hit
TGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATC12.857142857142857No Hit
ACTATAACAAGGCAACGAAGAGACTCCCAGTTCTAGGAAAGGATGCTGGA12.857142857142857No Hit
GGGCTGCAGGTCGAGGGATCTCCATAAGAGAAGAGGGACATCTATGACTG12.857142857142857No Hit
GTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATA12.857142857142857No Hit
GAAAAATGCTTCAATAATATTGAAAAAGGAAGAGAATGAGTATTCAACAT12.857142857142857No Hit
AATAAAACTTCTTCCCTTCGCAGCCGCTCCACCAAAGCAATGTAGAATGC12.857142857142857No Hit
GGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAG12.857142857142857No Hit
ACTGAAGGATGAGCTAATTCTCAGTCCCATTGCCGCCTTGCATATATCCA12.857142857142857No Hit
GCATTAATGAATCAGTGGATAACGCAGTAAAGAACATGTTAGCAAAAGGC12.857142857142857No Hit
CATGCTCACCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCTGTCCTCAG12.857142857142857No Hit
GGGAAAAAGATCTGCTAGCTAGAGCATGCACTGGAACCATGCAGGAAAAT12.857142857142857No Hit
CCCGGGCCACACGCAGGGGCCGGCCCGTCGAGAATTGACCTGCAGGGTCC12.857142857142857No Hit
ATTATTGCTTCGGCAATCGACTGTTCGTATCTCCAACTAACTATACTCTG12.857142857142857No Hit
GCAATAAACCCGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAAC12.857142857142857No Hit
TGTTGTGAGAAAGATGATGACTAATTCACAAGACACAGAGATTTCTTTCA12.857142857142857No Hit
GTGTACATTGAAGTGTTGCATTTGACTCAAGGAACATGCTGGGAACAGAT12.857142857142857No Hit
GATGCAGAGAGAGGCAAGTTAAAAAGAAGGGCTATCGCAACACCTGGGAT12.857142857142857No Hit
CCCAAATCACATAACGTGAGTTTTCGCTACACTGAGCGACATCCCCCGAA12.857142857142857No Hit
CTTGATGGTGGCGTACATGCTAGAAAGAGAATTGGTCCGTAAAACAAGGT12.857142857142857No Hit
GCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGT12.857142857142857No Hit
CCCCTCAGAACCGCGGACTCCAATACCGCTTTGCCGTCATATGGGTATGC12.857142857142857No Hit
GAAATGAATCAGTGTTAGTCAATAACGATAAATGGATAATCAGAAACTTT12.857142857142857No Hit
CAGCAAAATGGGTGTAGATGAGTACTCCAGCACGGAGAGGGTAGTGGTGA12.857142857142857No Hit
CCTCGGGCGACAGTAGTACATTTCCGCGTTGGTCCCTGCTTCGCGACCAC12.857142857142857No Hit
ATTGATAGGTATTGACCAACACTTATTCAGGTAAATTTATCTCCCACAGC12.857142857142857No Hit
ACCAAAGACATTGCAGTCTCAAAACAGCTCCCACCATTAGTGTGAAATCG12.857142857142857No Hit
GCCGGGGCAGGGCGGGGTTCGGCTTCTGGCGTGTGACCGGCGGCTATAGC12.857142857142857No Hit
TCCTCATTCTGGGCAAAGAAGACAAGAGATATGGGCCAGCACTAAGCATC12.857142857142857No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)