FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R1_E09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R1_E09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAGAGTGGTAGTGAGTATTGACCGATTTTTAAGGGTTAGAGATCAAAG22.631578947368421No Hit
CACGGAGGGAAGTCCACATATCTTACCTAGAGAAAGCCAACAAAATAGAA11.3157894736842104No Hit
CAACAGGGGAGAGGTAACAGTAACTATAATAAGATAGTGGTAGTAGGATT11.3157894736842104No Hit
TGGAGAAAACAAAGACCAAAACTAGCAGGAATCAAGGACTGAGAAGAACT11.3157894736842104No Hit
ATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACACAGCACTCAG11.3157894736842104No Hit
AGCTACAGGACATTGAAAATGAAGAGAAGATCCCAAGGACAAAGAACATG11.3157894736842104No Hit
CAAGTAGGCAGTTGCTGCCGGCGGGATCAGATAGAAGGGCAGACAGAAGG11.3157894736842104No Hit
GGGATGGGGGAAGGCCCAGAGATCTGCTACAATCCCCCCCGCAGAATCTT11.3157894736842104No Hit
CACTATGGATGGGAGAAGAGCAACAGCTAATCTCACAAAGCCAACAAGCA11.3157894736842104No Hit
ACCTGAGGATAATGAACCAAGTGGGTATGCACAAACAGACTGTGTTCTAG11.3157894736842104No Hit
CCCATTAGCATCTCTCTTGGAAATGTGCCACAGCACACAGATTGGAGGAG11.3157894736842104No Hit
GCACAGGATGTGATTATGGAAGTTGTTTTCCCAAATGAAGTGGGGGCAAG11.3157894736842104No Hit
TGAGGGAATACAAGCAGGAGTGGACAGATTCTACAGGACCTGCAAGTGAG11.3157894736842104No Hit
ATATAAAACATATTTCGAAAAGGTCGAAAGGTAGAAACATGGTACCTTCG11.3157894736842104No Hit
GTCGCTGAGAGGGATAAGAGTCAGCAAAATGGCAGTAGATGAATACTCCA11.3157894736842104No Hit
GGGGTATACCAACAACTGGAATGGGAACCATTGCAGTCTCTTTGACAGAA11.3157894736842104No Hit
CTTTCCCCTCATGCAGCACGCACAGGGCGTACGACCCGTTGTCGTTCCTG11.3157894736842104No Hit
GAACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTACCCGATTAGAGA11.3157894736842104No Hit
CTATGAAAGCCTGAGTAGGGAACTACATTCTCTTTATACATCGGAGGCAG11.3157894736842104No Hit
GGTGCTTTTCCAGAACTGGGGAATTGAATCCATCGACAATGTGATGGGAA11.3157894736842104No Hit
TTCCATGTTCACATTTGTCTCTTAAAAAACAAAACAGAATGAAAAAACAA11.3157894736842104No Hit
TTCCAGAACTGGGGAATTGAATCCATAGACAATGTGATGGGAATGATCGG11.3157894736842104No Hit
GGGCATCTTCCCTGGGCGGGAGATCATCCAGCCTACGGTGGTCGATCCTG11.3157894736842104No Hit
TTTCTTATCTTTGGTGCCCAATAATAATCAAGGGTTACAGAAAGAGACTT11.3157894736842104No Hit
GGCTAAAACAGAAGAGTCTTTAGAACTAGTACAACTCCAGTGAAATTTTT11.3157894736842104No Hit
TTGTGAATTCTTCATACCCTTCATGTACTCTTATTTTCAGTGTTTGGAGG11.3157894736842104No Hit
ATACTCATGTCATCTGATTCATTTACTCCAGAAACTCCAAAACTGGGTAG11.3157894736842104No Hit
GTGGAATAGGAATGGCCCAACAACAAGTACAGTTCATTAACCTAAGGTAT11.3157894736842104No Hit
GAGCAAGGGGGCTGAGATCTTGTCTCGGTTCATGAAACTGGTGAGGGCTG11.3157894736842104No Hit
GGATTGCATGATCAAGGCAGTTAGGGGCGATCTGAACTTTGTCAATAGGG11.3157894736842104No Hit
GAAGTGAACCCCAAAATTGAACCATTATTGAGGACGACACCACGCCCCCT11.3157894736842104No Hit
GCCTAACATAGTCAAACCACATGAGAAAGGCATAAATCCCAAGTCCCTCA11.3157894736842104No Hit
ACCATGGCCGCCACCATGCTAGAAATTCCACCCGGTCTCCTATATGAACT11.3157894736842104No Hit
GAGGAATATCCCGTCTATTCAATCTAGAGGCCTATTTGGGGCCATTGCCG11.3157894736842104No Hit
CTGTGGCCCACCCGCCACACAGCATTTCCTCGTCAGGAGCTGCCAGGGTC11.3157894736842104No Hit
GAGTAAATGAATCAGCTGACATTAGTATTTTATTAAAATTTAGAAAGACC11.3157894736842104No Hit
GACCAAACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGATTA11.3157894736842104No Hit
ATCTTTAACCGATTAGAGACCTTCATACTACTAAGGGATTTCACTGATGA11.3157894736842104No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTT11.3157894736842104No Hit
TTCTGTGGCCAGGCTTGGGGAAGGAGTACGATTTGATTCTGGAAATTATT11.3157894736842104No Hit
GTATGTTTCCCATTTGTGTGGCTCCAGTCTCGGGGCGGTGAGTGAGAACT11.3157894736842104No Hit
CTCCAACACCATGTCAAGCTTTCAGTTAGCCTGTATCCTTTGGCATATCC11.3157894736842104No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT11.3157894736842104No Hit
CTACCGGATCGACAAAGACAAGACGGGCAAGCTGTCCATCCCCGACGGGA11.3157894736842104No Hit
ACCGATTTGAGATAATTGAAGGAAGAGACCGAATCATGGCCTGGACAGTG11.3157894736842104No Hit
GTCTGGAGTAAATGAATCAGCTGACATGAGTATAGGAGTAAAAGTGATAA11.3157894736842104No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC11.3157894736842104No Hit
GTTAGAACACGATCGGCGATCAAGCATAGTGCATTTTGAAAAGCTGGTGC11.3157894736842104No Hit
ATGCAGCACGCACAGGGCGTACGACCCGTTGTCGTTCCTGTCCCTGATCC11.3157894736842104No Hit
ATTTTGATTGAAGTGCTGCTCTTGCTTTTCTTTTTTTCCTTTCTTTTGGA11.3157894736842104No Hit
CGGCCTTGCTCAATGCATCCGGTGCCGCCTTGGATGCCTGTCAGCTGATC11.3157894736842104No Hit
GACTCGTCCAGCTAGCCCTGCACCAGCTTTTCAAAATGCACTATGCTTGA11.3157894736842104No Hit
GCTAATGGGTCTGCTGACACTGCTGCTCTTCTTACTATGTTTCTAGCAGC11.3157894736842104No Hit
CAGGAGGATTGCATGATCAAGGCAGTTAGGGGCGATCTGAACTTTGTCAA11.3157894736842104No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA11.3157894736842104No Hit
CAGGTAGCGCCATCGTGGAGTTCCAAGCTGCATATGCCTGACCCTTCTGA11.3157894736842104No Hit
CTTATAATCACTTTGGAAGGCTTTTTAAAAACTCATTAACAGCTTGGGGT11.3157894736842104No Hit
CCGTAGACATATGCAAGGCAGCAATAGGGTTGAGGATAAGCTAATCTTTC11.3157894736842104No Hit
AACCCTGGCCATGCAGATCTCAGTGCCAAGGAGGCACAGGATGTGATTAT11.3157894736842104No Hit
GAGCAAGGCCGTATTTATGTACACTCCCTTAATTATGTATTCAGTAGCCC11.3157894736842104No Hit
CAGTAGGTAGGCTAAAACAGAAGAGTCTTTAGAACTAGTGCAACTCCAGT11.3157894736842104No Hit
GACTTAGAGGTTTAGAAATGTTAAATAGCTTGCCAGAGCTCCCCTTTTTT11.3157894736842104No Hit
GTTTTATTTTTGGTCCAGAGTACTTGTTTCCTGACTCGTCCAGCTAGCCC11.3157894736842104No Hit
CCACACAGCATTTCCTCGTCAGGAGCTGCCAGGGTCACCGAGCTGCCAGT11.3157894736842104No Hit
GTAGGCAAGGGAGAAGGGAGTCTCTGGGGCATGAGCAAGGGGGCTGAGAT11.3157894736842104No Hit
GGTTTAAAGTTACTAACAAATAAGAAGAACAAGGAAAATAACTCCTAGAT11.3157894736842104No Hit
GGATGCCGATCATTCCCATCACATTGTCGAGGGAGTCAATGAAACAGTTC11.3157894736842104No Hit
GTGTGAGCGGATGCAGTCGGGCCCTGGGGAGCAGGGCTGCGGTCCGTGGT11.3157894736842104No Hit
CCATCAGTCTTATAACTTCTGTTCGCATGTCGGATGTCCGTCCTTCATTG11.3157894736842104No Hit
GCATCCATCAGCAGGAACTTTGACCGCTGATGGCAAAGAGGCCCATCAGG11.3157894736842104No Hit
CAATTGCATCTGTACCTACTTCGCGCTACCTTTCTGACATGACCCTCGAG11.3157894736842104No Hit
ATAACACACAAGAAAAAAAAAAAATAAAAGAAAAGAAAAAACCAAAATAA11.3157894736842104No Hit
CTGTAAAATAGTTCCTCCACATGCTTGCGATATGTTCAATCGGCGCAACA11.3157894736842104No Hit
TAAAGAAGCTGTGGGATCAAACCCAATCAAAGGTAGGGCTATTAGTATCA11.3157894736842104No Hit
CCATTTGGTTAAGTTCAAAACTTCAATATAAACAATGCCTGTTCATACTT11.3157894736842104No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTTTG50.0145.07
TTTCTTA50.0145.01
TTATCTT50.0145.05
TATCTTT50.0145.06
TTCTTAT50.0145.02
CTTATCT50.0145.04
CTTTGGT50.0145.09
CTGCAGT50.0145.0145
TCTTTGG50.0145.08
TCTTATC50.0145.03
Produced by FastQC (version 0.11.5)