FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R1_E07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R1_E07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2186
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA90.41171088746569073No Hit
CATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTT60.2744739249771272No Hit
GTTTGGAGGTTGCCCGTTAGCACTTCTTCTTCTTTCTTGACTGATGATCC50.22872827081427266No Hit
ACACTAAGTGGAATGAAAATCAAAATCCTCGAATGTTCCTGGCGATGATT50.22872827081427266No Hit
GTCTTATTTATATAGGACTTCTTTTTGCTCATGTTGATTCCCACTAACTT50.22872827081427266No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG50.22872827081427266No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA50.22872827081427266No Hit
GTATTGGAGTAACAGTGATAAAGAACAACATGATAAACAATGACCTTGGA50.22872827081427266No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC50.22872827081427266No Hit
CCCCAGATGTGCCTTCATCTGGATCTTCAGTCAATGCACCTGCATCCTTT50.22872827081427266No Hit
GTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTG50.22872827081427266No Hit
GTGTACATAAATACGGCCTTGCTCAATGCATCCTGTGCAGCCATGGATGA40.18298261665141813No Hit
ATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGAGCCTA40.18298261665141813No Hit
TTCACTATGAGAGCAAAATCGTCGGATGATTGGAGCCCATCCCACCAGTA40.18298261665141813No Hit
TCCTATATGTGAGAACCAATGGAACCTCCAAGATCAAGATGAAATGGGGC40.18298261665141813No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGA40.18298261665141813No Hit
GGCCAACACCATAGAAGTCTTTAGATCGAATGGCCTAACAGCTAATGAGT40.18298261665141813No Hit
AGCTTGAACAGTCTGGGCTCCCAGTAGGGGGCAATGAAAAGAAGGCCAAA40.18298261665141813No Hit
CCCATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATGTT40.18298261665141813No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG40.18298261665141813No Hit
GAGTATTGGAGTAACAGTGATAAAGAACAACATGATAAACAATGACCTTG40.18298261665141813No Hit
GTAAGAGACAACATGACCAAGAAGATGGTCACGCAAAGAACAATAGGGAA40.18298261665141813No Hit
GCTAGAAACATAGTAAGAAGAGCAGCAGTGTCAGCAGACCCATTAGCATC40.18298261665141813No Hit
ATACTGATGTGGTGAACTTTGTAAGTATGGAGTTCTCACTCACTGACCCG40.18298261665141813No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC40.18298261665141813No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG40.18298261665141813No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT40.18298261665141813No Hit
TATTTAATGTCAGTGCTCTTATTAGATAGCCTCTCTTATTCAGTCTTTGT40.18298261665141813No Hit
TCTCAGTTCCTTGCGTTTCACTGACTTCTTCGGGAGACAATAGTACGTTC40.18298261665141813No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG40.18298261665141813No Hit
GTGGTGAACAGTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCT40.18298261665141813No Hit
TTCCTGAAGTCTGCTTAAAATGGGAGCTAATGGATGATGATTATCGGGGA40.18298261665141813No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG40.18298261665141813No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA40.18298261665141813No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC40.18298261665141813No Hit
CTATTAGCCTTCCTGACTCATTAGCTGTTAGGCCATTCGATCTAAAGACT40.18298261665141813No Hit
GGATAAGAGTCAGCAAAATGGGAGTAGATGAATACTCCAGCACGGAGAGA40.18298261665141813No Hit
GCATTGGCCAACACCATAGAAGTCTTTAGATCGAATGGCCTAACAGCTAA40.18298261665141813No Hit
GTCTGGAGTAAATGAATCAGCTGACATGAGTATTGGAGTAACAGTGATAA40.18298261665141813No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG30.1372369624885636No Hit
CCCTTCATTATGTATTCAGTAGCCCTGCAGTGGGACACTTCTGCTGTAAA30.1372369624885636No Hit
CTCCAATACTCATGTCAGCTGATTCATTTACTCCAGACACTCCAAAGCTG30.1372369624885636No Hit
GGCAATAATTGTGGCCATGGTATTCTCACAGGAGGATTGCATGATCAAGG30.1372369624885636No Hit
GCTTTGGAGTGTCTGGAGTAAATGAATCAGCTGACATGAGTATTGGAGTA30.1372369624885636No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCATGTCAAAG30.1372369624885636No Hit
GTATACGAAACCTCTAATCTGCATCCCAGGTGTTGCGATAGCCCTTCTTT30.1372369624885636No Hit
GAACATGGGCCTCGACACTTGGCCTATCGCAGTCCTCAAGAGCATGTCTC30.1372369624885636No Hit
TCTTTTTAACTTGCCTCTCTCTGCATCTTTGGTCATCGTATTTAATGTCA30.1372369624885636No Hit
CATTATGGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTC30.1372369624885636No Hit
GAGGAAATAGAGATAACAACCCACTTTCAAAGAAAAAGGAGAGTAAGAGA30.1372369624885636No Hit
ATTATTGCCTCAGCAATTGACTGCTCGTCTCTCCCGCTTACTATCAACTG30.1372369624885636No Hit
AACATAGTCAAACCACATGAGAAAGGCATAAATCCCAATTACCTCATGGC30.1372369624885636No Hit
GCTCAATGCATCCTGTGCAGCCATGGATGACTTTCAGCTGATCCCAATGA30.1372369624885636No Hit
GTGTTGGCCAATGCAGTTGCTGCCGGTTGATTTCTGTTTAATGTCCAATC30.1372369624885636No Hit
TTATGGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG30.1372369624885636No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA30.1372369624885636No Hit
GTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGA30.1372369624885636No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTT30.1372369624885636No Hit
ATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTGTATG30.1372369624885636No Hit
GACAAGAAATGGCCAGTAGGAGTCTATGGGATTCCTTTCGTCAGTCCGAA30.1372369624885636No Hit
CCTTGGACCTGCAACGGCCCAGATGGCTCTTCAATTGTTCATCAAAGACT30.1372369624885636No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA30.1372369624885636No Hit
CTTCAGGTCAATGCTTGCTAGCATTTCTGCTGGTATTTGTGTTCGAATCT30.1372369624885636No Hit
GTCAATTGCTGAGGCAATAATTGTGGCCATGGTATTCTCACAGGAGGATT30.1372369624885636No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC30.1372369624885636No Hit
GGCTAATAGATTTCTTAAAGGATGTAATGGAATCAATGAACAAAGAGGAA30.1372369624885636No Hit
TTGTAGAATCTGGTGACCCGAATGCACTATTGAAGCACCGATTTGAGATA30.1372369624885636No Hit
CACATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCAT30.1372369624885636No Hit
GATTGAACATATCGCAAGCATGAGGAGGAACTATTTTACAGCAGAAGTGT30.1372369624885636No Hit
GAGTTGATACAAACCCTGGCCATGCAGATCTCAGTGCCAAGGAGGCACAG30.1372369624885636No Hit
GTCCTGGGATGATGATGGGCATGTTCAACATGCTAAGTACGGTCTTGGGA30.1372369624885636No Hit
TCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATTTCAGAGCATCCA30.1372369624885636No Hit
GTACATAAATACGGCCTTGCTCAATGCATCCTGTGCAGCCATGGATGACT30.1372369624885636No Hit
TATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCTGG30.1372369624885636No Hit
GAATACCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGG30.1372369624885636No Hit
GTCTGGGCTCCCAGTAGGGGGCAATGAAAAGAAGGCCAAACTGGCAAATG30.1372369624885636No Hit
AAATAAGAGTACATGAAGGGTATGAAGAATTCACAATGGTTGGGAGAAGA30.1372369624885636No Hit
ATCATATTCCATGCTTTTGGCTGGACCATGGGCTGGCATTACCACAGCAT30.1372369624885636No Hit
GTATTGAAGACCCGAGTCACGAGGGGGAGGGAATACCACTATATGATGCA30.1372369624885636No Hit
CTAATAGCCCTACCTTTGATTGGGTTTGATCCCACAGCTTCTTTAACTCA30.1372369624885636No Hit
GTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAA30.1372369624885636No Hit
GCACTGGAGCTAGGATGAGTCCCAATAGTTCTCATTGCATGTACCATCTG30.1372369624885636No Hit
GTGTTCACGCTCACCGTGCCCAGTGAGCGAGGACTGCAGCGTAGACGCTT30.1372369624885636No Hit
CTATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGGGCCAAGGAGGT30.1372369624885636No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG30.1372369624885636No Hit
CATGAAGGGTATGAAGAATTCACAATGGTTGGGAGAAGAGCAACAGCTAT30.1372369624885636No Hit
GCAATAGGGTTGAGGATTAGCTCATCTTTCAGTTTTGGTGGGTTCACTTT30.1372369624885636No Hit
ACATGACCCCAAGCACGGAGATGTCGCTGAGAGGGATAAGAGTCAGCAAA30.1372369624885636No Hit
GTACTTAGCATGTTGAACATGCCCATCATCATCCCAGGACTCAGTGATGC30.1372369624885636No Hit
ATGTAATCATCGCCAGGAACATTCGAGGATTTTGATTTTCATTCCACTTA30.1372369624885636No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC30.1372369624885636No Hit
GGCAACCAGGAGATTGATCCAGTTGATAGTAAGCGGGAGAGACGAGCAGT30.1372369624885636No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG30.1372369624885636No Hit
CTCCAGGAGGAGAAGTGAGAAATGATGATGTTGACCAAAGTTTGATTATC30.1372369624885636No Hit
GAGACAACATGACCAAGAAGATGGTCACGCAAAGAACAATAGGGAAGAAA30.1372369624885636No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG30.1372369624885636No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT30.1372369624885636No Hit
GTTCCAGGTTGTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGAT30.1372369624885636No Hit
GTATAACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGT30.1372369624885636No Hit
CACCCATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATG30.1372369624885636No Hit
GCCGTAGACATATGCAAGGCAGCAATAGGGTTGAGGATTAGCTCATCTTT30.1372369624885636No Hit
GAACAATAGGGAAGAAAAAACAAAGACTGAATAAGAGAGGCTATCTAATA30.1372369624885636No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC30.1372369624885636No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCTCC100.0066095586145.07
AGAACTG100.0066095586145.05
Produced by FastQC (version 0.11.5)