FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R1_E01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R1_E01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCACATAAAATGGCCACAATTATTGCGTCGGCAATCGACGTTTCGTCGC15.263157894736842No Hit
ACTCCAGCTGTGCCTTCATCTGGGTCTTCAGTTAAAGTGCCAGCATCCTT15.263157894736842No Hit
CATTGCCGCCTTGCATATATCCACGGCTTGCTCTTCTGTTGGGTTCTGCC15.263157894736842No Hit
ACAAAGGGATACAGGGCATTACAAAGTCTACCCCGATAATCAACCTCCAT15.263157894736842No Hit
GGATGAAATGGATGATGGCAATGAAATATCCAATTACAGCAGACAAGAGG15.263157894736842No Hit
GGATGATGGCAATTTAACTGAAGACCCAGATGAAGGCAAAGCTGGAGTGG15.263157894736842No Hit
GTCAAGAGAGAGGAAGAGGTGCTTACGGGCAATCTTAAAACATTGAAGAT15.263157894736842No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG15.263157894736842No Hit
CCTCATTTACTGTGAATTTGAGGGGATCAGTAATTAGAATACTTGTAAGG15.263157894736842No Hit
TGGGACATAAGAAAAAAAGGCACAGAAAAAAAATACTTTAAATTATCCCG15.263157894736842No Hit
ATCACAGGGTCCTGGGAACGAGTCCCGCATCAGCATCTCAGATCAGCGCG15.263157894736842No Hit
CATCTGACTGCTTTTATCATACATTCCTCTTGTGAAAATACCCAGGCCAC15.263157894736842No Hit
TGATACTGCAGCTCTTCTCACTATGTTCCTAGCAGCAATAATCATTCTTT15.263157894736842No Hit
TCTGTGTGCTGTGGCACATCTCCAATAAAGATGCAATTGGATCATCTGAA15.263157894736842No Hit
TTACCAACACCCCGTCTCCTTGCCCAATTAGCACATTAGCCGTCTCTCCT15.263157894736842No Hit
GCCCAGAACCCTACAATGCTATACAATAAAATGGAATTTGACCCATTTCA15.263157894736842No Hit
CCGTGGGCGGCGTCTCACGCCTCTAATTTACATATCTAGGCTTGGAGGCA15.263157894736842No Hit
CCGTAATTCTGCATGCCCCTGTCTGGGGACCTGACTGCCTGGTGACAAAC15.263157894736842No Hit
GCATTGTAGGGTTATGGGACCACTGAATTTTAACAGTTTCCAAGTTTATT15.263157894736842No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)