FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD_S3_R1_D11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R1_D11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAGGGAAGTCCACATATATTACATAGAGAAAGCCAACAAAATAAAATC11.0869565217391304No Hit
AATCAACCCGAGTGGTTCAGAAACATCCTGAGCATGGCACCCATAATGTT11.0869565217391304No Hit
GTCTATATGAGTCTCCACAACATGAGGGCTTTTCGGCAGAATCTACAAAA11.0869565217391304No Hit
GCTACTGAATACATAATGAAGGGAGTGTACATAAATACGGCCTTGCTCAA11.0869565217391304No Hit
GGCAAACCATTTGAATCGATGTCAATCCGACTCAACTTTTCCTAAAAATT11.0869565217391304No Hit
ACTTTTTAAAGATAGCTCAGTTCATTGATTCTAAATGCTGGGCCATATCT11.0869565217391304No Hit
CATATAGACTGTAGAATACAGATTTTGCCAGTAAGGTCCTGCCCACTTTC11.0869565217391304No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCAAGCACACTTTC11.0869565217391304No Hit
GCTAGAAACATAGTAAGAAGAGCAGCAGTGTCAGCAGACCCATTAGCATC11.0869565217391304No Hit
TATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCATCTTC11.0869565217391304No Hit
GCTATGGATTTGTGGCTAATTTTAGCATGGAGCTACCCAGCTTTGGAGTG11.0869565217391304No Hit
CACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGACACTCGTGC11.0869565217391304No Hit
GGATGGGAACAGTGACCACAGAAGCTGCTTTTGGACTAGAGTGTGCCACT11.0869565217391304No Hit
ATATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTCATAGT11.0869565217391304No Hit
GGCAGAAAACAGCCTAACATAGTCAAACCACATGAGAAAGGCATAAATCC11.0869565217391304No Hit
TTCAACGCACCTGCATCCTTTCCAAGAACTGTAAGTCCTTTGGTTGACTT11.0869565217391304No Hit
CCTCAAAGCCGAGATCGCGCAGAGACTGGAAAGTGTCTTTGCAGGAAAGA11.0869565217391304No Hit
GTGGTAGTGCGTATTGACCGATTTTTAAGGGTTAGAGATCAAAGAGGGAA11.0869565217391304No Hit
GTACAGTTCATTACCCTAAGGTATATAAAACTTATATCGAAAAGGTCGAA11.0869565217391304No Hit
TCCAAAAAATTCCAGCGCAAAATGCCATAAGCACCACATTCCCTTATACT11.0869565217391304No Hit
GTCTCCCCTATGGCACCTATATGTGTATCTGTAGTCTTTGATGAACAATT11.0869565217391304No Hit
ATATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTCATACT11.0869565217391304No Hit
CCTGAAGTCTGCTTAAAATGGGAGCTAATCGATGATGATTATCGGGGAAG11.0869565217391304No Hit
GCCATGCAGATCTCAGTGCCAAGGAGGCACAGGATGTGATTATGGAAGTT11.0869565217391304No Hit
CCTACTGGCCATATCTAGTCTTATAGTCAAAAGCCTAGTTTTGATTCTTG11.0869565217391304No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC11.0869565217391304No Hit
GGTGCACCCCAGCTCACCCCGAGTGATGGACCCCTACCTGCGGATAAAGC11.0869565217391304No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG11.0869565217391304No Hit
CTATCATCCCGTCAGGCCACCTCAAAGCCGAGATCGCGCAGAGACTGGAA11.0869565217391304No Hit
AACTTATACAATATACGGAATCTTCACATACCTGAAGTCTGCTTAAAATG11.0869565217391304No Hit
GTGCTAGTCAACACTTATCAATGGATAATCAGGAACTGGGAAATTGTGAA11.0869565217391304No Hit
CAAATGTAGCACCAAAGAAGGAACACTGAAAACAAACATGTATTGGTTCA11.0869565217391304No Hit
ACATGACCCCAAGCTCGGAGATGTCGCTGAGAGAGATAAGAGACAGCAAA11.0869565217391304No Hit
GAAGTACTTCAATGAATCAACAAAGAAGAAAATTGAGAAAATAAGGCCTC11.0869565217391304No Hit
GGATAATGAACCAAGTGGGTATGCACAAACAGACAAATGACACCCCATGG11.0869565217391304No Hit
ACTTATTCGTCATCAATGATGTGGGAGCTCAATGTCCCTGAGTCAGTGCT11.0869565217391304No Hit
AACAAGTACAGTTCATTACCCTAAGGTATATAAAACTTATTTCGAAAAGG11.0869565217391304No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT11.0869565217391304No Hit
TCTCACAGGAGGATTGCATGATCAAGGCAGTTAGGGGCGATCTGAACTAT11.0869565217391304No Hit
TGACCACAATCCATACACCACCAATCTGTGTGATTTGGCAAAATTCCAAG11.0869565217391304No Hit
GGTGAACAGTATATGTAAAACAACAGGGGTAGAGAAGCCAAAATTTCTTC11.0869565217391304No Hit
GAAGATAACAATCCAATTGAAGTGGGCACTCGGTGAAAATATGGCACAAG11.0869565217391304No Hit
CAATAGCGAAGAAAAAACAAAGAATGAATAAGAGACCCTAACTAATAATA11.0869565217391304No Hit
GACCCCACTGATTGTGAAAGAAATCTCAGACTCTTGAGAATAAGTCATCA11.0869565217391304No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTCGAAAAAAGAATAGAGA11.0869565217391304No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT11.0869565217391304No Hit
CAGATATATGTAATCCTCGCCAGGAACATTCGACGATTTTGATTTTCATT11.0869565217391304No Hit
GGGGTAGAGAAGCCTAAATTTCTTCCTGATTTGTAATATTACAAAGAGAA11.0869565217391304No Hit
ATCTACATAGGCTCTAAAGTTTTCAAGTCTGGGGAAGTTCGGTGGGAGAC11.0869565217391304No Hit
CACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGT11.0869565217391304No Hit
AAACAGAACACACCAATACTCAGAAAAGGGAAAGTGCACGACAAACACAG11.0869565217391304No Hit
ACCAGAGCGATAAAAAAAGCTTGTGAATTCAAATGTCCATGACTTATTTA11.0869565217391304No Hit
TGATTGAGGCCGAGTCTTCTGTCAAAGAGAAAGACATGACCAAGGAATTC11.0869565217391304No Hit
CCCAATTACAGCAGACAAGAGAATAATGCACATGATTCCAGAGAGGAATG11.0869565217391304No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA11.0869565217391304No Hit
TTAAACATCAGCAAGGCAATAGAAGAAACCATCACTCATAATGAATTGGA11.0869565217391304No Hit
CGCAGGTGCTGCGGTGAAAGGAGTTGGAACATTAGCAATGGAGTTAATCA11.0869565217391304No Hit
GGAATATTTGAGAATTCATGCCTTGAAACAATGGAAGTTGTTCAACAAAC11.0869565217391304No Hit
GTGCCTGAACAATGAGAAGCAAATTTCTAGATTCAGCCGAAAACCCCTCA11.0869565217391304No Hit
CTATAAGACAAGAAATGGCCAGTAGGAGTATATGGGATTCCTTAAGTCAG11.0869565217391304No Hit
GGCCTGGACAGTGGTGAACAGTATATGTAACACAACAGCGGAAGAGAAGC11.0869565217391304No Hit
AATTATCACTGTTACTCCAAAACTCAAGTCAGCTGAATAATTTACACAAG11.0869565217391304No Hit
ACATTAAACAGTATGACAGAGATGAGCCATAGAACAGATCTCAAGCAATA11.0869565217391304No Hit
ACCATCAAGGCAGCAATTTTACAAACCTTGATCTATAAATTCTAGTCTTT11.0869565217391304No Hit
TCCTTGAAGAATCCCACCCAGGAATATTTGAGAATTCATGCCTTGAAACA11.0869565217391304No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG11.0869565217391304No Hit
TTCACTATGAGAGCAAAATCGTCGGATGATTGGAGCCCATCCCACCAGTA11.0869565217391304No Hit
GCCAGGACCATTCGAGGCTTTTGATTTTCATTCCACTTAGTGGTCTCCCG11.0869565217391304No Hit
GGGGATCGCAACAACTGGGATGCAGATTAGAGGTGTCGGCTACGTTGTTC11.0869565217391304No Hit
GTGGACGACAAACACAGAGACTGGTGCACCCCAGCTCACCCCCATTGATC11.0869565217391304No Hit
CACCAATACTCAGAAAAGCCAAAGTGGACGACAAACACAGAGACTGGTCC11.0869565217391304No Hit
GTCCACATAAATTACCTAGAGAAAGCCAACAAAATAAAATCTGAGAAGAC11.0869565217391304No Hit
CTTGATGGTGGCGTACATGCTAGAAAGAGAATTCGTCCGTAAAACAAGGT11.0869565217391304No Hit
GAATTAGTCATCATCGTTCTCACAACATTTGCCAGTTTGGCCTTCTTTTC11.0869565217391304No Hit
GTCCTATAAAAATAAGACAGGCACATTTGAATTCACAAGCTTTTTTTATC11.0869565217391304No Hit
CTTTGTTGAAACTTTAGCTAGGAGCATTTGCGAAAAGCTTGAACAGTCTG11.0869565217391304No Hit
ATCCCATAGACTCCTACTGGCCATTTCTTGTCTTATAGTGAAAAGCCTAG11.0869565217391304No Hit
GGATGTAATGGAATCAATGAACAAAGAGGAAATAGAGATAACAACCCACT11.0869565217391304No Hit
TTTCTGTTTAATGTCCAATCATAAGTCTGGCGACCTTGAGTTAGTTTATC11.0869565217391304No Hit
AAGTGCTAACGGGCAACCTCCAAACACTGAAAATAAGAGTACATGAAGGG11.0869565217391304No Hit
CCCATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGTGTACATGTT11.0869565217391304No Hit
ACACTAAGTGGAATGAAAATCAAAATCCTCGAATGTTACTGGCGAAGATT11.0869565217391304No Hit
ATGTAATCATCGCCACGAACATTCGAGGATTTTGATTTTCATTCCACATA11.0869565217391304No Hit
AGTCAGTGCTAGTCAACACTTATCAATGGATAATCAGGAACTGGGAAATT11.0869565217391304No Hit
ATATACTGCTATTCCCTCCCCCTCGTGACTCGGGTCATCAATCCTTAATT11.0869565217391304No Hit
CTGCAAAGATGTTGGAGACCTTAAACAGTATGACAGTGATGAGCCAGAGC11.0869565217391304No Hit
GTTAATCTATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGGGCCGG11.0869565217391304No Hit
GCTAATCCTCAACCCTATTGCTGCCTTGCATATGGCTACGGCTTGTTCCT11.0869565217391304No Hit
ATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGAGACAA11.0869565217391304No Hit
TAATCAGAATGATCAAACGTGGAATCAATGACCGAAATTTCTGGAGGGGT11.0869565217391304No Hit
CTTTATCACTGTTACTCCAATACTCATGTCAGCTGATTCATTTACTCCAG11.0869565217391304No Hit
ATTTAAGAGTATCAAGTTTCATAAGAGGAAAGAAAGTGATTCCAAGAGGA11.0869565217391304No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCGC50.0145.03
GATCGCA50.0145.04
CAACAAC50.0145.09
GCAACAA50.0145.08
ATAGATG50.0145.0145
TCGCAAC50.0145.06
GGGATCG50.0145.02
ATCGCAA50.0145.05
GGGGATC50.0145.01
CGCAACA50.0145.07
Produced by FastQC (version 0.11.5)