FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R1_D09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R1_D09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCCCCCCAGCTCAACCCGATTGATGGACCACTTCCTGAGGATAATGAA11.3157894736842104No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGTGAAACAAATCGTG11.3157894736842104No Hit
CTCTTGAGGACTGCGATGGGCCAAGTGTCGAGGCCCATGTTCCTATATGT11.3157894736842104No Hit
CTATTCAACTGGTGCACTTGCCAGTTGCATGGGCCTCATATACAACAGGA11.3157894736842104No Hit
CTACAGGACATTGAAAATGAAGAGAAGATCCCAAGGACAAAGAACATGAA11.3157894736842104No Hit
GAGAGAGTGGTAGTGAGTATTGACCGATTTTTAAGGGTTAGAGATCAAAG11.3157894736842104No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA11.3157894736842104No Hit
CCAAGTGGGTATGCACAAACAGACTGTGTTCTAGAGGCTATGGCTTTCCT11.3157894736842104No Hit
TGCATGGGCCTCATATACAACAGGATGGGAACAGTGACCACAGAAGCTGC11.3157894736842104No Hit
GAAGCCATTCTTTGGCTGGAAAGAGCCTAACATAGTCAAACCACATGAGA11.3157894736842104No Hit
GCTAATGAGTCAGGAAGGCTAATAGATTTCTTAAAGGATGTAATGGAATC11.3157894736842104No Hit
CAATGCAGCAGTTCGGATAGAATCCATCTACATAGGCTCTAAAGTTTTCA11.3157894736842104No Hit
CGGCTTCTGGTTGCCTTAGGGACAAGAGACTGAAATGGTTCAAATTCCAT11.3157894736842104No Hit
CACTACCACTCTCTCCGTGCTGGAGTATTGATCTCCTACCATTTTGCTGA11.3157894736842104No Hit
ACAAAATGGAATTTGAACCATTTCAGTCTCTTGTCCCTAAGGCAACCAGA11.3157894736842104No Hit
GTCCAGACTCGAAGTCGAACCTGGCATCAATCCGGGCCCTAGACACCATG11.3157894736842104No Hit
CTCCCCTATGGCACCTATATGTGTATCTGTAGTCTTTGATGAACAATTGA11.3157894736842104No Hit
ATGCAATTTTCCTCATTGACTGTGAATGTGAGAGGATCAGGGTTGAGGAT11.3157894736842104No Hit
GGTGAAAGGAGTTGGAACAATAGCAATGGAGTTAATCAGAATGATCAAAC11.3157894736842104No Hit
ACATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC11.3157894736842104No Hit
GCGCCATTCTGTCTTCTCTTTTGTTATTGCCATCGGTGACTCTGATGTCA11.3157894736842104No Hit
TCCCTTGGGTTAAGTGCAACACTTCAATATAAACACTGCCTTTTAAGCCG11.3157894736842104No Hit
GGTCCAAAATGAATTCAATAAGGAATGTGAATTGACTGATTCAAGCTTGA11.3157894736842104No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG11.3157894736842104No Hit
ACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGC11.3157894736842104No Hit
GTGCTCTCTAATGCAAGGTTCAACACTTCCAAGAAGGTCTGGTGCCGCAG11.3157894736842104No Hit
GTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTCCCAATAGAGC11.3157894736842104No Hit
CTCCAAAGCTGGGTAGCTCCATGCTAAAATTAGCCACAAATCCATAGCGA11.3157894736842104No Hit
GCACCCATAATGTTATCAAAAAAAATGGCAAGACTAGTGAAAGGGTACAT11.3157894736842104No Hit
GAAAAGATGTGAATGTGTGTCTTCTCAGATTTTATTTTGTTGGCTTTCTC11.3157894736842104No Hit
TCCTAATGTTAAATACATATTCTACACTGTAGATACCCATTAGAGCACAT11.3157894736842104No Hit
ATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAG11.3157894736842104No Hit
GAGAAGAAATAAATACCTAGAAGAGCATCCCAGTGCTGGGAAGGACCCTA11.3157894736842104No Hit
CTGTTTTCATGCCTGATTAGTGGATTGGTGGTAGTAGCCATCTGTCTGTG11.3157894736842104No Hit
TTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAA11.3157894736842104No Hit
ATACTGAATCTTGGACAAAATAAATACACCAAGACAATATACTGGTGGGA11.3157894736842104No Hit
CCTCAAGAGTTGGTGCATGGGGTTCAGTCGCTGGTTTGCCCTATTGACAA11.3157894736842104No Hit
GGTTTGTCCTTGTTCATTCCTCTCTGGAATCATGTCCATTATTCTCTTGT11.3157894736842104No Hit
ATGACGAGAGGATCACTTGAATCGCTGCATCTGCACTCCCATTCGCTTCT11.3157894736842104No Hit
GTATTCAACACTCAATATTCTTCTCCACAACTTGAGCTGTTTTCGGCTGA11.3157894736842104No Hit
ATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTGGAATGGATCTTG11.3157894736842104No Hit
GTAGCCCTGCAGTGGGACACTTCTGCTGTAAAATAGTTCCTCCTCATGCT11.3157894736842104No Hit
GCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCTGGTACATCTGTT11.3157894736842104No Hit
CACTAATCCACAATATCAGGTGCAAGATCCCAAAGAAATTTGCTGCAATG11.3157894736842104No Hit
GAGTAAGGATGGTGGACATCCTTAGACAGCATCCAACTGAGGAACAAGCC11.3157894736842104No Hit
GCTTCAATCCAATGATCGTCGAGCTTGCGGGAAAGGCAATGAAAGAATAT11.3157894736842104No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT11.3157894736842104No Hit
GTCAATTGCTGAGGCAATAATTGAGGCCATGGTATTCTCACAGGAGGATT11.3157894736842104No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT11.3157894736842104No Hit
GTCAATGAGGAAAATTGCATCCTACTCTGTTCTGGTGGGGCAGCAGCAAA11.3157894736842104No Hit
GTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGA11.3157894736842104No Hit
GCCATATTTTCACCGAGTGCCCACTTCAATTGGCTTGTTCTCTTCATGTT11.3157894736842104No Hit
TTTGAAAGTGAACCCAACAAAACTGAAAGATGAGCTAAACCTCAACCCTA11.3157894736842104No Hit
GGGTACATGTTCGAGAGTAAACAATTTAGCTTAGTACAAAAATTCCAAGA11.3157894736842104No Hit
CATCAGAGTCACAGCTGGCAATAACAAAAGAGAAGAAAGAAGAGCTCCAG11.3157894736842104No Hit
GCGCAAGCTTGCCGACCAAAGTCTCCCACCGAACTTCCCCAGCCTTGAAA11.3157894736842104No Hit
CAAGTACAGTTCATTACACTAAGGTATATAAAACTTATTTCGAAAAGGTC11.3157894736842104No Hit
CAGTCTGTTGAGCCACTGCCTTCTGCTCAGGTCCTGGAATCATTCCTGCA11.3157894736842104No Hit
TCTTGGAAGAGGAATCCAGCGAGACACTTAGAATGACAATTGCAACTGTA11.3157894736842104No Hit
GGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCAT11.3157894736842104No Hit
GCTTGAATCAGTCAATTCACATGCCTTATTGAATTCATTTTGGACCCAGC11.3157894736842104No Hit
GAGAGATGCTAATGTGTCTGCTGACACTGCTGCTCTTCTTACTATGTTTA11.3157894736842104No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG11.3157894736842104No Hit
GGGGTGCTACAAACCCTGGCCATGCAGCTCTCCGTGCCAAGGAGGCGCAG11.3157894736842104No Hit
AATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAGTTG11.3157894736842104No Hit
GCCATATCTATTGTCTTCTTTGCACAAAATGAGAAATCCTCTCAGGACAG11.3157894736842104No Hit
TCATTGGGATCAGCTGAAAGTCATCCATGGCTGCACAGGATGCATTGAGC11.3157894736842104No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATTATTTATT11.3157894736842104No Hit
CTCTTGGAAATGTGCCACCGCACCCAGATTGGAGGAGTAAGGATGGTGGA11.3157894736842104No Hit
ATTTTCACCCCTCCAGAAATTTCGGTCATTGATTCCACGTTTGATCATTC11.3157894736842104No Hit
GTAAAAATACTGTGGTCTGTAAATATGAGACATGCTATTGATGAATGAGA11.3157894736842104No Hit
GGACATACTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGG11.3157894736842104No Hit
GGGCAATAATCAAAACTATTATTGTAACTATACTACAAGAAATGGAAAGT11.3157894736842104No Hit
TAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGCCGC11.3157894736842104No Hit
ACCTAGAGAAAGCCAACAAAAAAAAATCTGAGAAGACACACATAAACATC11.3157894736842104No Hit
CATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTT11.3157894736842104No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGAAA50.0145.01
AGTGAAC50.0145.07
TTGAAAG50.0145.02
AAGTGAA50.0145.06
GTGAACC50.0145.08
AAAGTGA50.0145.05
TGAACCC50.0145.09
CATATCC50.0145.0145
GAAAGTG50.0145.04
TGAAAGT50.0145.03
Produced by FastQC (version 0.11.5)