FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R1_D07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R1_D07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2945
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA371.2563667232597622No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC301.0186757215619695No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG250.8488964346349746No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG220.7470288624787775No Hit
CTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCC180.6112054329371817No Hit
GGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCA160.5432937181663837No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG130.4414261460101867No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG120.40747028862478774No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG120.40747028862478774No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG110.37351443123938877No Hit
CATCACCTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATCTTCATGAT110.37351443123938877No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG110.37351443123938877No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT100.3395585738539898No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA100.3395585738539898No Hit
GCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAG100.3395585738539898No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA100.3395585738539898No Hit
AACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAG90.30560271646859083No Hit
CCTATTCCAGCGCAAAATGCCATAAGCACCACATTCCCTTATACTGGAGA90.30560271646859083No Hit
ATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGC90.30560271646859083No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA90.30560271646859083No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT80.27164685908319186No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTC80.27164685908319186No Hit
ACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTCATATAGCCCCCC70.23769100169779284No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG70.23769100169779284No Hit
CTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCA70.23769100169779284No Hit
GTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAA70.23769100169779284No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT70.23769100169779284No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG70.23769100169779284No Hit
CATTTGGAAGTTTGTTTCATGTATTCGATTTCCATTTCATCGACGAACGG70.23769100169779284No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG70.23769100169779284No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT60.20373514431239387No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA60.20373514431239387No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG60.20373514431239387No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC60.20373514431239387No Hit
GTCCTGGGATGATGATGGGCATGTTCAACATGCTAAGTACGGTCTTGGGA60.20373514431239387No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG60.20373514431239387No Hit
AAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAG60.20373514431239387No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG60.20373514431239387No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC60.20373514431239387No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT60.20373514431239387No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT60.20373514431239387No Hit
ATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG60.20373514431239387No Hit
TTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATCTTCATG60.20373514431239387No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC60.20373514431239387No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT60.20373514431239387No Hit
GCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAGAACACAC60.20373514431239387No Hit
CTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGG50.1697792869269949No Hit
ATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTGTATG50.1697792869269949No Hit
GATTTGAGATAATTGAAGGAAGAGACCGAATCATGGCCTGGACAGTGGTG50.1697792869269949No Hit
GAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACT50.1697792869269949No Hit
GTCTGGGCTCCCAGTAGGGGGCAATGAAAAGAAGGCCAAACTGGCAAATG50.1697792869269949No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC50.1697792869269949No Hit
ATCCAATGATCGTCGAGCTTGCGGAAAAGGCAATGAAAGAATATGGGGAA50.1697792869269949No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC50.1697792869269949No Hit
GAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATCATTAATCAGGCA50.1697792869269949No Hit
ATCTGAGGGGGCGTGGTGTCGTCCTCAAGAATGGTTCAATTTTGGCGTTC40.13582342954159593No Hit
CAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTG40.13582342954159593No Hit
GCCATAAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGG40.13582342954159593No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT40.13582342954159593No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC40.13582342954159593No Hit
GACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATGGCCTC40.13582342954159593No Hit
CTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTA40.13582342954159593No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA40.13582342954159593No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT40.13582342954159593No Hit
AAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCA40.13582342954159593No Hit
GGCCAACACCATAGAAGTCTTTAGATCGAATGGCCTAACAGCTAATGAGT40.13582342954159593No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC40.13582342954159593No Hit
TCCTAAAAATTCCAGCGCAAAATGCCATAAGCACCACATTCCCTTATACT40.13582342954159593No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTT40.13582342954159593No Hit
ATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCT40.13582342954159593No Hit
GTTCATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACAGC40.13582342954159593No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC40.13582342954159593No Hit
GCCACAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCA40.13582342954159593No Hit
GGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTG40.13582342954159593No Hit
GAACAGGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAA40.13582342954159593No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA40.13582342954159593No Hit
TCTGAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTT40.13582342954159593No Hit
TCCTTGATCGGCTCCGCCGAGATCAAAAGTCCTTAAAAGGAAGAGGCAAC40.13582342954159593No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT40.13582342954159593No Hit
CACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACTGGTGC40.13582342954159593No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT40.13582342954159593No Hit
GTGGTGAACAGTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCT40.13582342954159593No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA40.13582342954159593No Hit
GGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCCAATG40.13582342954159593No Hit
CTCTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAAT40.13582342954159593No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT40.13582342954159593No Hit
CAAGCCAAATTCATCACCTTTGCCGTCTGAGTTCTTCAATGGTGGAACAG40.13582342954159593No Hit
GTTCCAGGTTGTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGAT40.13582342954159593No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA40.13582342954159593No Hit
ATCCAATGATCGTCGAGCTTGCGGAAAAGGAAAGAATATGGGGAAGATCC40.13582342954159593No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC40.13582342954159593No Hit
GTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTG40.13582342954159593No Hit
CCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGG30.10186757215619693No Hit
ACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACAT30.10186757215619693No Hit
CTATTGAAGCACCGATTTGAGATAATTGAAGGAAGAGACCGAATCATGGC30.10186757215619693No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA30.10186757215619693No Hit
ATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCG30.10186757215619693No Hit
AAGATGCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATT30.10186757215619693No Hit
GTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATTTCAGA30.10186757215619693No Hit
CCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAA30.10186757215619693No Hit
CAGTAATCCACAATATCAGGTGCAAGATCCCAATGATATTTGCTGCAATG30.10186757215619693No Hit
CGCATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTT30.10186757215619693No Hit
GGAACAGGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGA30.10186757215619693No Hit
GAAATAAATACCTAGAAGAGCATCCCAGTGCTGGGAAGGACCCTAAGAAA30.10186757215619693No Hit
GTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTCCCAATAGAGC30.10186757215619693No Hit
GAACAGGAACAGGATACACCATGGACACAGTAAACAGAACACACCAATAC30.10186757215619693No Hit
GTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAAACCCCTCA30.10186757215619693No Hit
GTCTTGGGAGTCTCGATACTGAATCTTGGACAAAAGAAATACACCAAGAC30.10186757215619693No Hit
GTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGA30.10186757215619693No Hit
TTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCA30.10186757215619693No Hit
CTTCAGGAATGTGAAGATTCCGTATATTGTATAAGTTTGGTCCTCCATCT30.10186757215619693No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATT30.10186757215619693No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA30.10186757215619693No Hit
GTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGA30.10186757215619693No Hit
CAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAG30.10186757215619693No Hit
GTGTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTT30.10186757215619693No Hit
ATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTC30.10186757215619693No Hit
ACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATC30.10186757215619693No Hit
CTTCTGGTAGGCCTGCAAATTTTCAAGAAGGTCATCTTTCAGACCAGCAC30.10186757215619693No Hit
CCCATATATAGAAGAGTAGACGGAAAGTGGATGAGAGAACTCATCCTTTA30.10186757215619693No Hit
GTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTCAGT30.10186757215619693No Hit
ATTAGTATCAGATGGAGGACCAAACTTATACAATATACGGAATCTTCACA30.10186757215619693No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA30.10186757215619693No Hit
GACTGAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATG30.10186757215619693No Hit
GTGCTCTCTAATGCAAGGTTCAACACTTCCCAGAAGGTCTGGTGCCGCAG30.10186757215619693No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC30.10186757215619693No Hit
CTTTCCCAATAGAGCCTTCCTCCACTCCCCTGGGTGACTCTCCGATTGGC30.10186757215619693No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG30.10186757215619693No Hit
ATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGA30.10186757215619693No Hit
ACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGCGGAAAAG30.10186757215619693No Hit
GGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATC30.10186757215619693No Hit
CCATAAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGA30.10186757215619693No Hit
GACCGAATCATGGCCTGGACAGTGGTGAACAGTATATGTAACACAACAGG30.10186757215619693No Hit
TCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATAT30.10186757215619693No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT30.10186757215619693No Hit
ATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCC30.10186757215619693No Hit
CTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAA30.10186757215619693No Hit
CTACAGGACCTGCAAGTTAGTGGGAATCAACATGAGCAAAAAGAAGTCCT30.10186757215619693No Hit
TGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATAA30.10186757215619693No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTA30.10186757215619693No Hit
ATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCA30.10186757215619693No Hit
GTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTTGAGTTAA30.10186757215619693No Hit
ACTGAATACATAATGAAGGGAGTGTACATAAATACGGCCTTGCTCAATGC30.10186757215619693No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG30.10186757215619693No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA30.10186757215619693No Hit
GCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGA30.10186757215619693No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT30.10186757215619693No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG30.10186757215619693No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCG30.10186757215619693No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC30.10186757215619693No Hit
TAATTGTAGAATCTGGTGACCCGAATGCACTATTGAAGCACCGATTTGAG30.10186757215619693No Hit
ATCAGGCACTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCC30.10186757215619693No Hit
GGATGATGGCAATGAGATAATGGACATGATTCCAGAGAGGAATGAACAAG30.10186757215619693No Hit
AGGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGC30.10186757215619693No Hit
CCCCAGATGTGCCTTCATCTGGATCTTCAGTCAATGCACCTGCATCCTTT30.10186757215619693No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT30.10186757215619693No Hit
CAATTGAAGAGCCATCTGGGCCGTTGCAGGTCCAAGGTCATTGTTTATCA30.10186757215619693No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT30.10186757215619693No Hit
CAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCA30.10186757215619693No Hit
ATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACT30.10186757215619693No Hit
GTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAG30.10186757215619693No Hit
GCGGAAAAGGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAA30.10186757215619693No Hit
GTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCTAGAAAA30.10186757215619693No Hit
AAACTAGGCTTTTCACTATAAGACAAGAAATGGCCAGTAGGAGTCTATGG30.10186757215619693No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATCTG254.910643E-429.0140-144
ATCTGTT200.00588174929.0140-144
Produced by FastQC (version 0.11.5)