FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD_S3_R1_D04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R1_D04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGCTCTGCTAGCACCTGCTTCCAAGCCATGAGGTAATTGGGATTTA11.2658227848101267No Hit
GACATGATTCAAGAGAGGAATGAACAAGGACAAACCCTCTGGAGCAAAAC11.2658227848101267No Hit
GAATGCAGAACATCTAGTACATCTCTTCAGAATCGAAAATTCACCTTTCG11.2658227848101267No Hit
CAAAAGGACAAGCGGATCATCAGTCAAGAAAGAAGAAGAAGTGCTAACGG11.2658227848101267No Hit
TAAATACGATGACCAAAGATGCAGAGAGAGTCAAGTTAAAAAGAAGGGCT11.2658227848101267No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC11.2658227848101267No Hit
GTGGTCCTCAGTAAAACTTTAGAACTTAAATTTCATCAATAACAAAATGT11.2658227848101267No Hit
ATTCTCATCACAGTTTCTCCAAGCGAATCTCTGTATATTTTCAGAGTCGC11.2658227848101267No Hit
CTTTGACAGAAGACTCGGCCTCAAACATTCTCTCAATCTGCTGAAGAGAC11.2658227848101267No Hit
ACTTCAATCAAACAACAAAGAATAAAATTGACAAAAAAATTCCTCATATA11.2658227848101267No Hit
GCATAATGCTGGCCCTCTTGTCTTCTTTGCCAAAAATCAGAAATCCTCTC11.2658227848101267No Hit
TTGCATATGTCTACGGCTTGTTCCTCAGTTGGATTCTGTCTAAGGATGTC11.2658227848101267No Hit
CTCCAACATCTTTGCAGTCATCAAAGTCTACTTTTTCTGGTGCCACATTT11.2658227848101267No Hit
CACTAAGAATCACAATAGCATCACTACCTACTACGCGCAACCTTTCTCAC11.2658227848101267No Hit
CAAACAGACTGTGTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCC11.2658227848101267No Hit
CCTGAGCATGGCACCCATAATGTTCTCAAACAAAATGGCAAGACTAGGGA11.2658227848101267No Hit
CAATAGAAGTGGGCACAAGGTGAAAAAATGGCACCAGAAAAAGTATACTT11.2658227848101267No Hit
CAGAATAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA11.2658227848101267No Hit
CTAAACATCAGTAAGGCAAGAGAAGAACCAATCACTCAGAATGAAGAGCA11.2658227848101267No Hit
GAACAGGATACACCATGACACAGAAAACAGAAAACACAAATACTCAGAAA11.2658227848101267No Hit
AGACAATATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTC11.2658227848101267No Hit
CAATCAAATGCATGAAGACATTCTTTCGCTGGAAAGAGCCTAACATAGTC11.2658227848101267No Hit
CAACATGACCAAGAAGATGGTCACGCAAAGAACAATAGGGAAGAAAAAAC11.2658227848101267No Hit
GCCTAAATTTCATCCTGATTAGTATGATTACAAAGAGAACCGGTTCATTG11.2658227848101267No Hit
GGATGGTGGACATCCTAAGACAGAATCAAACTGAGGAACAAGCCGTAGAC11.2658227848101267No Hit
GACAATATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTCA11.2658227848101267No Hit
CAATCGGGGCAACATCTTCTCCTATTTCATCAAGTTCTATCCAGCTTGAA11.2658227848101267No Hit
GGATCTTGTGACCATTGAATTTTCTCAATTTCCCAGTACCTGATTATCCA11.2658227848101267No Hit
GAATTCTTCATACCCTTCATGTACTCTTATTATCAGAGTATGGAGCTTGC11.2658227848101267No Hit
ATAATCAAACTTTGGTCAACATCATCATTTCTCACTTCTCCTCCTGGAGT11.2658227848101267No Hit
CTCCATACTTACAAAGTTCACCACATCAGTATCATTTCTCAAATCAGAAC11.2658227848101267No Hit
GTGCACTATCCCCAAGCCAAGCCCAGTTATACAACAGTAGGTGAACCGTT11.2658227848101267No Hit
AACTATTAGAATAGTCCATAATTTCTAAATTTTCTAATTTGTTCATTCAT11.2658227848101267No Hit
GATGTAATGGAATCAATGAACAAAGAGGAAATAGAGATAACAACCCACTT11.2658227848101267No Hit
GTGTTACACCAAATTCAAAGAACCGGTTCACATTGTAATCATACAAATCA11.2658227848101267No Hit
AAATGACAAAAGAACTCAACGCCAAAATTGAACCATTCTAGAGGACGACA11.2658227848101267No Hit
GCTATGGGGCATTCACCATCCACCTACTAGTGATGAACAACAAAGTCTCT11.2658227848101267No Hit
ATAAGACCCAAAGTGAGGGATCAAGAAGGGAGAATGAACTATTACTGGAC11.2658227848101267No Hit
GCTCTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAA11.2658227848101267No Hit
CTTACAGGACATACTTATGAGGATCTCAAAAATGGAGTTGAGGTCCTCAT11.2658227848101267No Hit
GAATAGAACGATTCCTCTTGGGAATCCAGGAATGTGTAGTACCAACGGCA11.2658227848101267No Hit
CTCTGGAGCAAAACAAACGATGGTAAGAGGCAATACACCAGTATTCAATA11.2658227848101267No Hit
TTCCTAAAAAATCCAGCGCAAAATGCCATAAGCACCACATTCCCTTATAC11.2658227848101267No Hit
GGCATTACCACAGCATTGTTTACAGAATCAATCTCTTTATGACTGACAAA11.2658227848101267No Hit
TCTCTTGTCATCTTTGCCCAAAATGAGAAAACCTCTCAGCACACCAGACT11.2658227848101267No Hit
CCCATAATGTTCTAAAACAAAATGGCAAGACTAGGGAAAGGGTACATGTT11.2658227848101267No Hit
ACTCTGATGTCAGTATTCTTGCCCCCACTTCATTTGGGACACCAACTTCC11.2658227848101267No Hit
ATACAATATACCGAATCATCACATTCCTGAAGTCACCTTAAAATCTTTCA11.2658227848101267No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT11.2658227848101267No Hit
ATTCCGATCATTCCCATCACATTGACGATCGATTCAATTCCCCAGATCAC11.2658227848101267No Hit
GGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATCATTAATC11.2658227848101267No Hit
GGGGTGTAGGAGTCTTTGGGATTCCTTTCGTCAGTCCGAAAGAGGCGAAG11.2658227848101267No Hit
TTCCTGAAGTCAGCTTAAAATGGGAGCTAATGGATGATGATTATCTGGGA11.2658227848101267No Hit
AGATTGCAGCACTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTG11.2658227848101267No Hit
GTGGACCATATGGCCATAAAAAAAAAGTACACATCAGGAAGGCAACAGAA11.2658227848101267No Hit
CACCTATAATGTTCTCAAACAAAATGGAAAGACTAGGGAAAGGGTAAAAG11.2658227848101267No Hit
GGATTGGGTGATGCCCCATTCCTTGATAGGCTCCGCCGAGATCAAAACTC11.2658227848101267No Hit
GTCACTGTTTACACCACAAAAGGATATGCTGCTCCCGCTAGTCCAGATTG11.2658227848101267No Hit
GTTGAATGGGTAGCTATAAACTATTGACCGGCCTAGTGTAGACAGACGCA11.2658227848101267No Hit
GCCATGGAACAGGATCAGGATACCCCATGGACACAGTAAACAGAACACAC11.2658227848101267No Hit
AACCCATACAGGTTTGTTTTCCGTCTACCTTCTTTGGTCCTACATTTGCT11.2658227848101267No Hit
AGATCAAAGAGGGAACGTACTATTGTCTCCCGAAGAAGTCAGTGAAACGC11.2658227848101267No Hit
TCATTTAAAAGAACATAGTACTGAAAGAGAACTACAGAGTAATCTTTAAC11.2658227848101267No Hit
CCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTGTTGATATAT11.2658227848101267No Hit
AGACACAGAGATTTCTTTCACAATCACTGGGGACAACACTAAGTGGAATG11.2658227848101267No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC11.2658227848101267No Hit
ATTTTAAACAATTCGACACTAATTGATGGCCAACCGAATTCTTTTGGTCC11.2658227848101267No Hit
AAACAAACAAGGGTAGATAAACTAACTCAAGGTCGCCAGACTTATGATTG11.2658227848101267No Hit
TTGCTGAGCTTTGGGTATGAATTTCCTTTTTTAACTAGCCATATTAAATT11.2658227848101267No Hit
ATTAGTAGAAACAAGGTCGTATTTAAACAATTATACACTAAAAGATGGCA11.2658227848101267No Hit
GTTCAAATTCCATTTTGTTGTATAACATTGTGGGATCTTGTGACCATTGA11.2658227848101267No Hit
GTCCTGGGATGATGATGGGCATGTTCAAGATGCTTAGTACGGTCTTGGCA11.2658227848101267No Hit
GAATGGCCCAGCATTAAGCATCAATGAACAGAGCAATCTTGCAAAACCAG11.2658227848101267No Hit
TCATTATGTATTCAGTAGCCCTGCAGTGGGACACAACTGCTGTAAAATAG11.2658227848101267No Hit
CTAGAAGAATACTCCAGCACGGAGAGAGTGGTACTGAGTATTGACCGATT11.2658227848101267No Hit
GTCCTATATAAATAAGACAGGGACATTAGAATTCACAAGCTTTTTTTATC11.2658227848101267No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTAGACACTAATTGATGGCCAACC11.2658227848101267No Hit
GGATAATGAACCAACTGGCTAAGCACAAACAGACTGTCATCTACATGCTA11.2658227848101267No Hit
GCTAGAAACATAGTAAGAAGATCAGCAGTGTCAGCAGACCCATTAGCAAC11.2658227848101267No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTGGA50.0145.01
TCTGGAG50.0145.02
TGGAGCA50.0145.04
GAGCAAA50.0145.06
GCAAAAC50.0145.08
CTGGAGC50.0145.03
TCTCCTG50.0145.0145
AGCAAAA50.0145.07
GGAGCAA50.0145.05
CAAAACA50.0145.09
Produced by FastQC (version 0.11.5)