FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R1_D01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R1_D01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGGCAGGTGAAGCAGGGCACGTGGAACAGCTGCCCCAGAGCCCGGACC13.8461538461538463No Hit
AAGCAAAGGCCCTTGTGTCCCTGCAGATGCTAGGAAAACTCTCACCTCTT13.8461538461538463No Hit
AAAACAAGGAGGGGAACAAAAGGTCGAGGTGGCAGAGGGGAAATGAATGG13.8461538461538463No Hit
GTGCGCTAGGAAGTGGCCCGAAGGAGGTCAGGAGTGTCATTCATTCCCTC13.8461538461538463No Hit
TAAGAAAACTGCGGCACAGACCAGCTAAGTAACCTGTGCAAAGTCACAGA13.8461538461538463No Hit
CATTAGAGCAGTATAAGAAGCTGAATAGAGCAAGAAGGCAGAAAGCAGAC13.8461538461538463No Hit
CAGTTAAACAACATTCAAGTAAAAACATTATAATTTATTGATCAAAAAAA13.8461538461538463No Hit
CTCCCTAAACACTAAGGTACACACCGCAATTAGGTTAAAGCTTCTCCCCC13.8461538461538463No Hit
ACCGCATAGTTCCTGTATTCTAATATTTTACTTGAAATCTCAGATCGGCT13.8461538461538463No Hit
CTTTGGTTCCTTGACCCCAGTGGGCCTCACTCCTGACACCTAGTTCCCAA13.8461538461538463No Hit
ATTATTAATTTCAATAGCATTGAATAGTAAATAGTTGAAGTTAATTATTG13.8461538461538463No Hit
ATCCGGGCCCTAGACACCATGGCCTCCAACCAGCTAGAAATTCCACCCGC13.8461538461538463No Hit
GTACATTATGCTGTGCTCACGGCAGGGGTAGCTACGGTCCCACCATATGG13.8461538461538463No Hit
GCCCTGGAGTCCCCCAGTGGCAAGCGCACAACCTCTGGCTCCTGGTCCAG13.8461538461538463No Hit
GCTCGTGGTTCCTGACGCAACGCTCCGAAAGCTCGTGTAATTTCCTGATA13.8461538461538463No Hit
GCCCAAAGGGTAGTTTATATTAGAAAGGAGGGGAATGAAAGAGAAAATAG13.8461538461538463No Hit
CGCAAACACTGTTATTAAATAAGAAATTCAATAGAATTGTCTGTAATTAG13.8461538461538463No Hit
TGTGCTCCCAGCGGTGACCCAGCACTCACAACAATTCCGCTGCGGGGTTA13.8461538461538463No Hit
CTTATTGGTGGCGGGTTTTGCTCTGCTCCCACCAGGAGACTGCAGCAGTG13.8461538461538463No Hit
GTCGATGTGCTGTGATGTGCGGCTTAGGGATCGGGAAGGGTGTGAGATTG13.8461538461538463No Hit
GAACAGCTTCAGGATGTGTCTGATAAGGGAGGTGTAATGGTGCACCGGCG13.8461538461538463No Hit
CCTCTATGATTACTTACTTCTGGGTCTGTTCACCCCTCCACTCAGTCTCC13.8461538461538463No Hit
GAGTTTAAAGTGGCTTACCCCAAGTCACACAACGAGTGAGTGGCAGAGCC13.8461538461538463No Hit
GCCTGCCGCCGGGGCCGCCTGAAGCAGTGACCCCATGCCGCGCCCCTCAC13.8461538461538463No Hit
TATACACAGAATGTGGCTAAAAAATGTGTTCAGAGGTATAGGCTGGCACA13.8461538461538463No Hit
GCCATGATGTCATGGGGCATAATCATACGGCTGTTCAGAAACTCTGCACG13.8461538461538463No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)