FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R1_C09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R1_C09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGCTCTGCTAGCACCTGCTTCCAAGCCATGAGGTAATTGGGATTTA11.1363636363636365No Hit
CTTGAGGCTCTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCT11.1363636363636365No Hit
GGTCTAGCGGCTTTGGCTTTCCTTGAAGAATCCCACCCAGGAATATTTGA11.1363636363636365No Hit
ATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTTCTCAGTTCATTGAT11.1363636363636365No Hit
CAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAACCTTCTAGAA11.1363636363636365No Hit
CATCCTGTGCCTCCTTGGCACTGAGATCTGCATGGCCAGGGTTTGTATCA11.1363636363636365No Hit
TAACAAAAGAGAAGAAAGAAGAGCTCCAGGATTGTAAAATTGCTCCCTTG11.1363636363636365No Hit
GACAAATCTTCTGGCTTTGCACTTTCCATCATTCTTATAACTTCTGTTCG11.1363636363636365No Hit
TATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCTGG11.1363636363636365No Hit
GAATTCTTCATACCCTTCATGTACTCTTATTTTCAGTGTTTGGAGGTTGC11.1363636363636365No Hit
GTATATGTAACACAACAGGGGTAGAGAAGCCAAAATTTCTTCCTGATTTG11.1363636363636365No Hit
CAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTCTCTATTTTCT11.1363636363636365No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG11.1363636363636365No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT11.1363636363636365No Hit
TAAAAGAACTGAGATATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC11.1363636363636365No Hit
GTAATCCACAATATCAGGTGCAAGATCCCAATGATATTTGCTGCAATGAC11.1363636363636365No Hit
ATCCACTAATCAGGCATGAAAACAGAATGGTGCTGGCTAGCACTACGGCA11.1363636363636365No Hit
CGCTTCTGGTAGGCCTGCAAATTTACAAGAAGGTCATCTTTCAGACCAGC11.1363636363636365No Hit
ATACTCAGAAAAGGGAAAGTGGACGACAAACACAGCACCCCAGCTCAACC11.1363636363636365No Hit
AATCAACAAAGAAGAAAATTGAGAAAATAAGGCCTCTTCTAATAGATGGC11.1363636363636365No Hit
CTTTCCATCATTCTTATAACTTCTGTTCGCATGTCGGATGTCCGGCCTTC11.1363636363636365No Hit
ATCTGATACTAATAGCCCTACCTTTGATTGGGTTTGATCCCACAGCTTCT11.1363636363636365No Hit
CAAATGTCCCTGTCTTATTTATATAGGACTTCTTTTTGCTCATGTTGATT11.1363636363636365No Hit
CTATTGTTCTTTGCGTGACCATCTTCTTGGTCATGTTGTCTCTTACTCTC11.1363636363636365No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCATGTCAAAG11.1363636363636365No Hit
TCAATGTCCTGTAGCTCTGCTAGCACCTGCTTCCAAGCCATGAGGTAATT11.1363636363636365No Hit
TTGTTGGAGATACCTCATTCAACGTCGATGTCCAAGCTGTCGTGAGAGAT11.1363636363636365No Hit
GGATAAGAGTCAGCAAAATGGGAGTAGATGAATACTCCAGCACGGACAGA11.1363636363636365No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG11.1363636363636365No Hit
CTTGTCACCTCTGACTAAGGGAATTTTAGGATTTGTGTTCACGCTCACCG11.1363636363636365No Hit
CCCTAGTCTTGCCATTTTGGTTGAGAACATTATAGGTGCCATGCTAATGA11.1363636363636365No Hit
TGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGT11.1363636363636365No Hit
CCCCTAATCAGGCATGAAAACAGAATGGTGCTGGCTAGCACTACGGCAAA11.1363636363636365No Hit
CATCCCACCAGTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGT11.1363636363636365No Hit
TATAACTTCTGTTCGCATGTCGGATGTCCGTCATTAATTGTTACATCAGA11.1363636363636365No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA11.1363636363636365No Hit
GAATATGGTCAAGAAACGAAAATCCAAAATAACAAGTTTTCTCAAATAAC11.1363636363636365No Hit
GGATACACCATGGACACAGTAAAAAGAACACACAAATACTCAGAAAAGGG11.1363636363636365No Hit
GGATAATCAGGAACTGGGAAATTGTGAAAATTAAATGGTCACAAGATCCC11.1363636363636365No Hit
TCAGTAATCCACAATATCAGGTGCAAGATCCCAATGATATTTGCTGCAAT11.1363636363636365No Hit
CTTCATGTACTCTTATTTTCAGTGTTTGGAGGTTGCCCGTTAGCACTTCT11.1363636363636365No Hit
CAAAAGAAGAAGTTCAACAAACAAGGTTAGATAAACTAACTCAAGGTCCC11.1363636363636365No Hit
CAATAAAGCAGATAAAAGCAAGTATTGTCAGGCATGAAATGGAAACCAAA11.1363636363636365No Hit
GGCTTTGAAAGGGAAGGGTACTCACTGGTCGGGATAGACCCATTCAAATT11.1363636363636365No Hit
GTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACGGAAGTTCGCTTTC11.1363636363636365No Hit
ATATTTTACAGCAGAAGTGACCCACTGCAGGGCTACTGAATACATAATGA11.1363636363636365No Hit
GGTCTTCAATACTTAATTTCAGAGCATCCATCAGCAGGAACTTTGACCGC11.1363636363636365No Hit
AAAATACTGTGTTCTTGAAATAGGAGACATGCTCTTGAGGACTGCGATAG11.1363636363636365No Hit
ATTTTACAGCAGAAGTGTCCCACTGCAGGGCTACTGAATATAAAATCAAG11.1363636363636365No Hit
TAGATAAGTGTTGACTAGAACTGACTCAGGGCCATTGATCTCCAACATCA11.1363636363636365No Hit
GTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATTTCAGCGCA11.1363636363636365No Hit
GGTCTATCCCGCTTGAATCAGTCAATTCACATGCCATAATGAATTCAATT11.1363636363636365No Hit
GTTCCAAGGGGGATGTGTTTGTCATAAGGGACCCATTCATATCATGCTCC11.1363636363636365No Hit
ATAAAAGGAAGGTCTCATTTGAGAAATGATACTGATGTGGTGAACTTTGT11.1363636363636365No Hit
TTCCGAGAGAGATGCTAATGGGTCTGCTGACACTGCTGCTCTTCTTACGA11.1363636363636365No Hit
CTCCTGGTCGTTTAGGCCCACTAAGTGGTTGTCTGTTGCACGGTATAGGT11.1363636363636365No Hit
ACATTAAATACGATGACCAAAGATGCAGAGAGAGGCAAGTTAAAAAGAAG11.1363636363636365No Hit
CTTGAATCGCTGCATCTGCACTCCCATTCGCTTCTGCTAGGCATGCAAAT11.1363636363636365No Hit
CTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCA11.1363636363636365No Hit
GGATACACCATGGACACAGTAAACAGCCCACACCAATACTCAGAAAATGG11.1363636363636365No Hit
CAATGACCTTGGACCTGCAACGGCCCAGATGGCTCTTCAATTGTTCATCA11.1363636363636365No Hit
GCTAATGGGTCTGCTGACACTGCTGCTCTTCTTACTATGTTTCTAGCAGC11.1363636363636365No Hit
GAAAAGAACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATT11.1363636363636365No Hit
GGGCCATTGATCTCCCACATCATTGATGACGAATAAGTTATTGTCAACTT11.1363636363636365No Hit
CCCATTCGCTTCTGGTAGGCCTGCAAATTTTCAAGAAGCTCATCTTTCAG11.1363636363636365No Hit
GTCTCGGGTCAGTGAGTGAGAACTCCATACTTACAAAGTTAACCACATCA11.1363636363636365No Hit
TTTTAAGGGTTAGAGATGAAAGAGGGTACGTACTATTGTCTGCCGAAGAA11.1363636363636365No Hit
CCTCTAATCTGCATCCCAGGTGTTGCGATAGCCCTTCTTTTTAACTTGCA11.1363636363636365No Hit
GCCTTGAAAACTTTAGAGCCTATGTAGATGGATTCGAGCCGAACGGCTGC11.1363636363636365No Hit
AAGATGACCTTCTTGAAAATTTGCAGGCCTAACAGAAGCTAAAGGGAGTG11.1363636363636365No Hit
CTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGAGCC11.1363636363636365No Hit
CTCAATCTGCTGAAGAGACTGAAGAAGGCAGCGCCTCATTTCCATGCCCC11.1363636363636365No Hit
ATTATGTCCTGGAAGAGAATGTAATGGTGAAATTTCTCCAACTATTGCTA11.1363636363636365No Hit
GGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGA11.1363636363636365No Hit
GTCCTATTGTCTCCCGAAGAAGTCAGTGAAACGCAAGGACCTGAGAAGTT11.1363636363636365No Hit
GCCTAACATAGTCAAACCACATGAGAAAGGCATAAATCCCAATTACATCA11.1363636363636365No Hit
GTCTGTGAGACCGATGCTGTGAATCAGCAATCTGTTCACAAGTGGCACCC11.1363636363636365No Hit
TTCCTCAACTGTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCT11.1363636363636365No Hit
CTTTCAGGTAGACTGTTTCCTTTGGCATATCCGCAAGCGAATTGCAGACA11.1363636363636365No Hit
CTTATGAAACTTGATACTCTTAAATCTTCAAATGCAGCAGAGTGGCATGC11.1363636363636365No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC11.1363636363636365No Hit
ATATAAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTAT11.1363636363636365No Hit
GTTCAATTTTGGCGTTCACTTCTTTTGACATTTGGGAAAGCTTGCCCTCA11.1363636363636365No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACAACTGTGGCCCATATGTCCA11.1363636363636365No Hit
CCTTAAACAGTATGACAGTGATGAGCCAGAGCCCAGATCTATAGCAAGAT11.1363636363636365No Hit
CTCTGCTGTTCCTGTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTC11.1363636363636365No Hit
AGCAGGTACTGATCCAAAATGGAATACGTTGTGAGACAATTCTTCACTCC11.1363636363636365No Hit
GACTCGGCCTCAATCATGCTCTCAATCTGCAGAAGAGACTGAAGAAGGCA11.1363636363636365No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCATG50.0145.01
TTCATGT50.0145.02
GTACTCT50.0145.07
ACTCTTA50.0145.09
TCATGTA50.0145.03
ATGTACT50.0145.05
TGTACTC50.0145.06
TACTCTT50.0145.08
ATATTTC50.0145.0145
CATGTAC50.0145.04
Produced by FastQC (version 0.11.5)