FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R1_C05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R1_C05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACTGGTGC17.6923076923076925No Hit
GTGCTAGTCAACACTTATCAATGGATAATCAGGAACTGGGAAATTGTGAA17.6923076923076925No Hit
CACTCCAGGAGGAGAAGTGATAAATGAGGATGTTGACCAAATTTTGATTA17.6923076923076925No Hit
CCTTTGGCATATCCGCAAGCGATTTGCAGACAATGGATTGGGTGATGCCC17.6923076923076925No Hit
CCCTATTGCTGCCTTGCATATGTCTACGGCTTGTTCCTCAGTTGGATTCT17.6923076923076925No Hit
AGAATACCATGGCCACAATTATTGCCTCAGCAATTGACTGCTCGTCTCTC17.6923076923076925No Hit
CTGGTGTTCTCAATATATTGTTTATCCAACTCTTGAGAGTGTCTGTTATT17.6923076923076925No Hit
TGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAAACCCCTCAA17.6923076923076925No Hit
GTGATGCCCCATTCCTTGATCGGCTCAGCCGAGATCAAAAGTCCTTAAAA17.6923076923076925No Hit
AGGAGAAACAAGGAAGTTTTTCATGAAGTAAAATTTAAATTCAGTATTTT17.6923076923076925No Hit
CTGGAGTATTCATCTACTCCCATTTTGCTCACTCTTATCCCTCTCAGCGA17.6923076923076925No Hit
GGAGAGTTGATACAAACCCTGGCCATGCAGATCTCAGTGCCAAGGAGGCA17.6923076923076925No Hit
GACTGGTGCACCCCAGCTCAACCCGATTGATGGACCACTACCTGAGGATA17.6923076923076925No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)