FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD_S3_R1_B11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R1_B11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACTGGTGC22.7027027027027026No Hit
CCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTTGAGTTAAAGA22.7027027027027026No Hit
CTCCTGCTTGTATTCCCTCATGGTTTGGTGCATTCACTATGAGAGCAAAA11.3513513513513513No Hit
ACCTGCAACGGCCCAGATGGCTCTTAAATTGTTCAACAAAGACTAAAGAT11.3513513513513513No Hit
GAAAGAAATCTCTGTGTCTTGTGAATTAGTCATCATCTTTCTCACAACAT11.3513513513513513No Hit
GTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTG11.3513513513513513No Hit
ACCATCAAGGGAGCAATTTTACAATCCTGGAGCTCTTCTTTCTTCTCTTT11.3513513513513513No Hit
CTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACGGACCAATTCTCTT11.3513513513513513No Hit
AAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCA11.3513513513513513No Hit
CTTCTAATATATGGCACAGCATCACTGAGTCCTGGGATGATGCTGGGCAT11.3513513513513513No Hit
GAGTTGGAACAATAGCAATCGAGTTAATCAGAATGATCAAACGTGGAATC11.3513513513513513No Hit
GTCGGGCAGTATTCCGATCATTCCCATCACATTGTCGATGGATTCAATTC11.3513513513513513No Hit
GACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGATAATCATCATCCA11.3513513513513513No Hit
GTTATCTCTATTTCCTCTTTGTTCATTTATTCCATTACATAATTTAAGAA11.3513513513513513No Hit
CTTCATGTTCTTTGTCCTTGGGATCTTCTCTTCATTTTCAATGTCCTGTA11.3513513513513513No Hit
ACCCAGCTTTGGAGTGTCTGGAGTAAATGAATCAGCTGACATGAGTATTG11.3513513513513513No Hit
TAGTAAGCGGGAGAGACGAGCAGTCAATTGCTGAGGCAATAATTGTGGCC11.3513513513513513No Hit
ATAGAAGTCTTTAGATCGAATGGCCTAACAGCTAAAGAGTCAGGAAGGCT11.3513513513513513No Hit
CCCATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATGTT11.3513513513513513No Hit
ATTGAATCCATCGACAATGTGATGGGAATGATCGGAATACTGCCCGAAAT11.3513513513513513No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTCGGAGAAACCTTGTTTTAAG11.3513513513513513No Hit
CTCCAAAACACATGACAGCTGAATCATATACTCCACACACTCCAAAGCTA11.3513513513513513No Hit
CCATTTACACATGCACATTCAGACTCTTGTGTTCTCAATATATTGTTTCT11.3513513513513513No Hit
ATTGAAGCACCGATTTGAGATAATTGAAGGAAGAGACCGAATCATGGCCT11.3513513513513513No Hit
GGCAATGAGATACCCAATTACAGCAGACAAGAGAATAATGGACATGATTC11.3513513513513513No Hit
GTCCACATATATTACCTAGAGAAAGCCAACAAAATAAAATCTGAGAAGAC11.3513513513513513No Hit
TCTTGAGGCTCTCATGGAATGGCTAAAGACAAGCCCAATCTTGTCACCTC11.3513513513513513No Hit
AGACTGTTGAATACAGATTTTGCCACAAAGGACCTCCACACTTTCCCAAT11.3513513513513513No Hit
CCGCTGATGGCAAAGAGGCCCATCAGGCAATCTGAGGGGGCGTGGTGTCG11.3513513513513513No Hit
ACATTTTCTTATCATTGGTATCAGCTGAAAGTCATCAATCGCTTAACAGT11.3513513513513513No Hit
GTATCAAGGTCTCTAATCGGTTAAAGATTACACTTAAGTTCGCTTTCAGT11.3513513513513513No Hit
CCTTCCGTAGAAGGCCCTCTTTTAAAACCGTATATAAAGCGAAGATAAAT11.3513513513513513No Hit
ACATCCACAGCACTCTGCTGTTCCTGTTGATATTCTTCCCTCATGGCCTC11.3513513513513513No Hit
GAGTAACAGTGATAAAGAACAACATGATAAACAATGACCTTGGACCTGCA11.3513513513513513No Hit
ATATAAACACTGCCTGTTACGCCGGCTACTGGGAGACACCTTGTTTTACG11.3513513513513513No Hit
CTATCGGACTGACGAAAGGAATCCCATAGACTCCTACTGGCCATTACTTG11.3513513513513513No Hit
GAGCTACCCAGCTTTGGAGTGTCTGGAGTAAATGAATCAGCTGACATGAG11.3513513513513513No Hit
AGATGGCACAGCATCACTGAGTCCTGGGATGATGATGTGCATGTACAACT11.3513513513513513No Hit
GAAATACACCAACACAAAATACTGGTGGGATGGGCTCCAATCATCCGACG11.3513513513513513No Hit
CGTTAGCACTTCTACTTCTTTCTTGACTGATGATCCGCTAGTCCTTTTGA11.3513513513513513No Hit
CCTGAATCCCTTTGTCAGTCATAAAGAGATTGATTCTGTAAACAATGCTG11.3513513513513513No Hit
GATAGAGCAGTTAAACTATACAAGAAGCTAAAAAGAGAAATAACGTTCCA11.3513513513513513No Hit
GGCCCGTCCGGCACTCTGAGGGGGCGTGGTGACGTCCTCATGCATGCTTC11.3513513513513513No Hit
ACCTTAAACAGTATGACAGTGATGAGCCAGAGCCCAGATCTCTAGCAAGC11.3513513513513513No Hit
GTTCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATTCTGTTTTGATGC11.3513513513513513No Hit
TTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCAG11.3513513513513513No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA11.3513513513513513No Hit
TCATGTACTCTTATATTCAGTCTTTTGAGGTTGCCCGTTTTCACTTCTTC11.3513513513513513No Hit
GTATTCATCTACTCCCATTTTGCTGACTCTTATCCCTCTCAGCGACATCT11.3513513513513513No Hit
ACCTTGTTTTACGGACCAATTCTCATTCTAGCATGAACGCCACCATCAAG11.3513513513513513No Hit
TTGTTAGCCAATTGATGCCATCATGACTAGCACTTGCTGCCCAAGAGTCT11.3513513513513513No Hit
GCCCCTAACTGCCTTGATCATGCAATCCTCCTGTGAGAATACCATGGCCA11.3513513513513513No Hit
GGTCCTACATTTGCTTATCATTGGGATCAGCTGAAAGTCATCCATGGCTG11.3513513513513513No Hit
GTGGAGCAGATCTTCATCATCTCAGAGAACTCTTCTTTCTTGATCCGTCC11.3513513513513513No Hit
CACTAAGACCACTGTGGACCATATGGCCATAAACAAAAAGTACACATCAG11.3513513513513513No Hit
GTATGCATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG11.3513513513513513No Hit
GATTGCAGCACTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGG11.3513513513513513No Hit
AAGACTGAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGA11.3513513513513513No Hit
AAAATGAGGCGCTGCCTTCTTCAGTCTCTTCAGCAGATTGAGAGCATGAT11.3513513513513513No Hit
GTCAAACCACATGAGAAAGCCATAAATCCCAATTACCACATGGCTTGGAA11.3513513513513513No Hit
GTGTTGAGAATAGAACGATTCCTCTTGGGAATCCAGGAATGTGTAGTTGC11.3513513513513513No Hit
CCTCCAAACACTGAAAATAAGAGTACATGAAGGGTATGAAGAATTCACAA11.3513513513513513No Hit
GTCCTGGGATGATGATGGGCATGTTCAACATGCTAAGTACGGTCTTGGGA11.3513513513513513No Hit
ATAATACCTAGAGAAAGCCAACAAAATAAAATCTGAGAAGACACACATTC11.3513513513513513No Hit
CGCATATAGACTGTTGAATACAGATTTTCCCAGTAAGGTCCTGCACACTT11.3513513513513513No Hit
CAATGTGATGGGAATGATCGGAATACTGCCCGACATGACCCCAAGCACGG11.3513513513513513No Hit
ACTTACTTTTAAAATCAAACCATACATATTTGTTGTCATTCTTACTTATT11.3513513513513513No Hit
GTGAGAGAGGATCAGGGTTGAGGATACTGGTAAGAGGCAATTCTCCAGTA11.3513513513513513No Hit
CCTGCACACTTTCCCAATAGAGCCTTACTCCAAACCCCTCGGTCACTCTC11.3513513513513513No Hit
TGTGAGGGGGCGTGGTGTCGTCCTCAAGAATGGTTCATTTTTGGCGTTGC11.3513513513513513No Hit
ATATAAATAAGACAGGGACATTTGAATTCACAAGCTATTTTTATCGATAT11.3513513513513513No Hit
GAGGAACTATTTTACAGCAGAAGTGTCCCACTGCAGGGCTACTGAAAACA11.3513513513513513No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATGGG50.0145.06
GATGGGA50.0145.07
CAATGTG50.0145.01
ATGGGAA50.0145.08
ATGTGAT50.0145.03
GGTTAGA50.0145.0145
GTGATGG50.0145.05
TGGGAAT50.0145.09
AATGTGA50.0145.02
TGTGATG50.0145.04