FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R1_B07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R1_B07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3099
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTACACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA230.7421748951274605No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA210.6776379477250726No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA170.5485640529202969No Hit
CTTACACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACC130.41949015811552115No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG120.3872216844143272No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG110.35495321071313324No Hit
CACCCATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATG110.35495321071313324No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA100.32268473701193934No Hit
GTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTG100.32268473701193934No Hit
GTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCC90.29041626331074544No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT90.29041626331074544No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC90.29041626331074544No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG90.29041626331074544No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG80.2581477896095515No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTT80.2581477896095515No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT80.2581477896095515No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT80.2581477896095515No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC80.2581477896095515No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT70.22587931590835753No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG70.22587931590835753No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG70.22587931590835753No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC70.22587931590835753No Hit
TTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCGAAAAGCTTGA70.22587931590835753No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG70.22587931590835753No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC70.22587931590835753No Hit
CATTATGGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTC60.1936108422071636No Hit
CTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGATAA60.1936108422071636No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG60.1936108422071636No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA60.1936108422071636No Hit
ATCCGGGCCCTAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGG60.1936108422071636No Hit
CAGTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATT60.1936108422071636No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG60.1936108422071636No Hit
TTTCTGAACCACTCGGGTTGATTTCTGGTGATATATGTAATCATCGCCAG60.1936108422071636No Hit
CAATTGAAGAGCCATCTGGGCCGTTGCAGGTCCAAGGTCATTGTTTATCA60.1936108422071636No Hit
ATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGAGCCTA50.16134236850596967No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG50.16134236850596967No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGA50.16134236850596967No Hit
ATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCT50.16134236850596967No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC50.16134236850596967No Hit
GAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAA50.16134236850596967No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC50.16134236850596967No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG50.16134236850596967No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTC50.16134236850596967No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA50.16134236850596967No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC50.16134236850596967No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG50.16134236850596967No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT50.16134236850596967No Hit
AATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA50.16134236850596967No Hit
TTCCTGAAGTCTGCTTAAAATGGGAGCTAATGGATGATGATTATCGGGGA50.16134236850596967No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT50.16134236850596967No Hit
CAGTAGGGGGCAATGAAAAGAAGGCCAAACTGGCAAATGTTGTGAGAAAG50.16134236850596967No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA50.16134236850596967No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT50.16134236850596967No Hit
CCCTTCATTATGTATTCAGTAGCCCTGCAGTGGGACACTTCTGCTGTAAA40.12907389480477574No Hit
GTTCAAGCTTTTCGCAAATGCTCCTAGCTAAAGTTTCAACAAAGTATACG40.12907389480477574No Hit
GGATACACCATGGACACAGTAAACGAGGAATCGTTCTATTCTCAACACAA40.12907389480477574No Hit
GTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTCCCAATAGAGC40.12907389480477574No Hit
TAAGGACACTGTTCCAGCAAATGCGGGATGTGCTTGGGACATTTGACACT40.12907389480477574No Hit
GTTTTATTATTTGGACAGTGTCAAATGTCCCAAGCACATCCCGCATTTGC40.12907389480477574No Hit
GGATAGAACTTGATGAAATAGGAGAAGATGTTGCCCCGATTGAACATATC40.12907389480477574No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA40.12907389480477574No Hit
ACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTG40.12907389480477574No Hit
GGCCCTAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCC40.12907389480477574No Hit
GGCTAATAGATTTCTTAAAGGATGTAATGGAATCAATGAACAAAGAGGAA40.12907389480477574No Hit
GAATAGAACGATTCCTCTTGGGAATCCAGGAATGTGTAGTTGCAACGGCA40.12907389480477574No Hit
ATGTAGATGGATTCGAGCCGAACGGCTGCATTGAGGGCAAGCTTTCCCAA40.12907389480477574No Hit
GTCTGGGCTCCCAGTAGGGGGCAATGAAAAGAAGGCCAAACTGGCAAATG40.12907389480477574No Hit
GTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTTGA40.12907389480477574No Hit
ATGAAGGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGG40.12907389480477574No Hit
CTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCA40.12907389480477574No Hit
GTTAAAGATTACACTGAAGTTCGCTTTCAGTACTATGTTCTTTTCCATGA40.12907389480477574No Hit
GGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTC40.12907389480477574No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC40.12907389480477574No Hit
TATATTACCTAGAGAAAGCCAACAAAATAAAATCTGAGAAGACACACATT40.12907389480477574No Hit
GAGTAACAGTGATAAAGAACAACATGATAAACAATGACCTTGGACCTGCA40.12907389480477574No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA40.12907389480477574No Hit
GTGGTGAACAGTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCT40.12907389480477574No Hit
GTTTAAGGTCTCCAACATCTTTGCAGTCATCAAAGTCTACTTTTTCTGGT40.12907389480477574No Hit
GTGTAGTTGCAACGGCATCATATTCCATGCTTTTGGCTGGACCATGGGCT40.12907389480477574No Hit
GAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATCATTAATCAGGCA40.12907389480477574No Hit
CAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGG40.12907389480477574No Hit
GTTCCAGGTTGTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGAT40.12907389480477574No Hit
ATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATA40.12907389480477574No Hit
CCACATATATTACCTAGAGAAAGCCAACAAAATAAAATCTGAGAAGACAC40.12907389480477574No Hit
GCCGTAGACATATGCAAGGCAGCAATAGGGTTGAGGATTAGCTCATCTTT40.12907389480477574No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC40.12907389480477574No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTT100.006750369145.05
TTCGTAT100.006750369145.01
GTATACT100.006750369145.04
CGTATAC100.006750369145.03
TCGTATA100.006750369145.02
ATACTTT100.006750369145.06
TACTTTG100.006750369145.07
Produced by FastQC (version 0.11.5)