FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R1_B05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R1_B05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAAGACAAGAAATGGCCAGTAGGAGTCTATGGGATTCCTTTCGTCAG19.090909090909092No Hit
GAGCATGGATTGTCTTCCCGTTTGTTTGTGCCATCTTCAATTTCTTTCAT19.090909090909092No Hit
CGTGCTAGATCCGTTCAGACACTCTCACCCTTCCTGACTAAGACGATAGT19.090909090909092No Hit
CTGTGACATATTTTAAAAATCGTCTAATATTTAATAAAGTTCTATCCATC19.090909090909092No Hit
ATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACATGGTAACTTCG19.090909090909092No Hit
CCGGACTTAGCATGTTGAACATGACCCTCATCATACAAGGCATCATTGAT19.090909090909092No Hit
ACTGAATACATAATGAAGGGAGTGTACATAAATACGGCCTTGCTCAATGC19.090909090909092No Hit
GCCTTGCTCAATGCATCCTGTGCAGCCATGGATGACTTTCATCTGATAGC19.090909090909092No Hit
GTAAAATTGCTCCCTTGATGGTGGCGTACATGCTAGAAAGAGAATTGGTC19.090909090909092No Hit
GCTAGATGAAATATCCATTGTAAACATTTACACACCTAATAATAGACCAT19.090909090909092No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCCGCACTTCT19.090909090909092No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)