FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R1_A11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R1_A11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1309882
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG40390.30834838557976973No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT33830.25826753860271384No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG28360.21650805187032113No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT27930.21322531342517875No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA27460.2096372039618836No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA27270.20818669162565787No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC26970.20589640898951203No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA26450.2019265857535259No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA23500.17940547316475836No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC23220.17726787603768887No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC22530.17200022597455344No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC22380.1708550846564805No Hit
GTATAACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGT21710.16574012010242145No Hit
ATGTTATACAACAAAATGGAATTTGAACCATTTCAGTCTCTTGTCCCTAA20420.15589190476699427No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT19440.14841031482225117No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG19190.14650174595879628No Hit
TTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCGAAAAGCTTGA18910.14436414883172682No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT17550.1339815342145323No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG15990.12207206450657387No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT15610.11917103983412247No Hit
GAACTGGGGAATTGAATCCATCGACAATGTGATGGGAATGATCGGAATAC15600.11909469707958426No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG15600.11909469707958426No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG15420.11772052749789676No Hit
GATTAGAGGTTTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCG15260.11649904342528564No Hit
CATTAAATACGATGACCAAAGATGCAGAGAGAGGCAAGTTAAAAAGAAGG14750.11260556294383768No Hit
ATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACATGGTACCTTCG14740.11252922018929949No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG14290.10909379623508073No Hit
ACTTAGTGTTGTCCCCAGTGATTGTGAAAGAAATCTCTGTGTCTTGTGAA14270.10894111072600433No Hit
CATTATGGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTC14230.10863573970785155No Hit
CATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTT14220.10855939695331336No Hit
GTACTATTGTCTCCCGAAGAAGTCAGTGAAACGCAAGGAACTGAGAAGTT14140.10794865491700778No Hit
GTCTGGGCTCCCAGTAGGGGGCAATGAAAAGAAGGCCAAACTGGCAAATG14090.10756694114431682No Hit
ACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACAT13840.10565837228086195No Hit
CTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGATAA13760.10504763024455639No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG13540.10336808964471608No Hit
ATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGAGCCTA13490.10298637587202511No Hit
ACACTAAGTGGAATGAAAATCAAAATCCTCGAATGTTCCTGGCGATGATT13480.10291003311748692No Hit
CAATAGTACGTTCCCTCTTTGATCTCTAACCCTTAAAAATCGGTCAATAC13470.10283369036294872No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC13320.10168854904487579No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT13210.10084877874495565No Hit
CCCCACTTCTGGAAAAGCACTTTTGCATCTTTTTGGAAATGCCTCAAGAG13180.10061975048134106No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGAA2900.0112.533511
AGCGAAA900.0104.753421
GGTACTG1800.0104.717435
CGAAAGC900.0104.717433
CAGGTAC1850.0101.887223
GCGAAAG1000.094.2528842
GCAGGTA1950.092.951552
GACAGTA3600.088.6070567
AGGCCGG259.3791465E-487.01927145
ATATAAT851.8189894E-1276.787571
ATTATTA406.6566485E-572.51606145
ATTATAT809.822543E-1172.496683
GTAGAAA5750.070.629721
AGGTACT2750.068.542314
TTATAGG10050.063.5014951
TAGATAT808.092684E-963.4345973
AGCGAGC350.003563622762.15662145
GCAGGGT702.335837E-762.140018
CGATCCC350.003567397662.140015
GAAAGCA1550.060.8036654
Produced by FastQC (version 0.11.5)