FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R1_A05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R1_A05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTGCTCCCTCCTCTGTGAAAGCACTTAGTAGTATCAAGGTCTCTAAT14.545454545454546No Hit
TTATAAAAGGAAGGTCTCATTTGAGAAATGATACTGATGTTGTGAACTGT14.545454545454546No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT14.545454545454546No Hit
GTAAAATAGTTCCTCCTCATGCTTGCGATATGTTCAATCGGGGCAACATC14.545454545454546No Hit
GCTTGGAAGCAGGTGCTAGCAGAGCTACAGGACATTGAAAATGAAGAGAA14.545454545454546No Hit
CATTTGAGAAATGATACTGATGTGGTGAACTTTGTAAGTATGGAGTTCTC14.545454545454546No Hit
CAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCG14.545454545454546No Hit
ACAGATTGCTGATTCACAGCATCGGTCTCACAGACATATTGCTACTACCA14.545454545454546No Hit
GCTACTGGGAGAAACCTTGTTTTACGGACCAATTCTCTTTCTAGCATGTA14.545454545454546No Hit
CCCCCTGCACCAACTCTTGAGGCATTTACAAAAAGATGCAAAAGTGCCAG14.545454545454546No Hit
CCCAGCACGTCCCTTGGGTTAAGTGCAACACTTCAATATAAACACTGCCT14.545454545454546No Hit
TCCCAATAGAGCCTTCCTCCACTCCCCTGGGTGACTCTCCGATTGGCCAT14.545454545454546No Hit
CATTCGTTGTATGGAAAGAGACTAACATATTAAAACCAAATTAGCAAGTA14.545454545454546No Hit
GCCCTGAGTCAGTGCTAGTCAACACTTATCAATGGATAATCAGGAACTGG14.545454545454546No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG14.545454545454546No Hit
GCCACAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCA14.545454545454546No Hit
GGAGAAGATGTTGCCCCGATTGAACATATCGCAAGCATGAGGAGGAACTA14.545454545454546No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC14.545454545454546No Hit
AATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTAC14.545454545454546No Hit
GTTCTGGTGGGGCAGCAGCAAAGGGGAGAAGTTTTATTATTTGGACAGTG14.545454545454546No Hit
GACCATGGGATGGCATTCCCAAAGCATTGGTGACAGAATCAATCTCTTGA14.545454545454546No Hit
ATCAGCTCCTTCCCAAACAGAGAATACGTGTTGCTCAACTCCGTCCCTAA14.545454545454546No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)