FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R1_A04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R1_A04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences934225
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA28360.30356712783323075No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG23780.25454253525649606No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT22810.24415959752736227No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG21460.22970911718269155No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA20940.22414300623511468No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT18820.20145040006422435No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA18730.200487034707913No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC18370.19663357328266745No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC18030.19299419304771334No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC17990.19256603066713052No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA16960.1815408493671225No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG16500.17661698199041986No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT16280.17426208889721428No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC15480.16569884128555756No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG15460.1654847600952661No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT15420.16505659771468328No Hit
ATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGAGCCTA14740.15777783724477507No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC14500.15520886296127806No Hit
CTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGATAA14400.15413845700982098No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG14350.15360325403409242No Hit
GTATAACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGT14260.15263988867778105No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG13060.13979501726029597No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG12280.13144585083893068No Hit
TTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCGAAAAGCTTGA12230.1309106478632021No Hit
CATTAAATACGATGACCAAAGATGCAGAGAGAGGCAAGTTAAAAAGAAGG11850.12684310524766518No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT11830.12662902405737375No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT11250.12042066953892264No Hit
ACACTAAGTGGAATGAAAATCAAAATCCTCGAATGTTCCTGGCGATGATT11070.11849393882629987No Hit
GATTAGAGGTTTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCG10940.11710241108940567No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG10640.11389119323503438No Hit
GTATACGAAACCTCTAATCTGCATCCCAGGTGTTGCGATAGCCCTTCTTT10570.11314190906901442No Hit
ATGTTATACAACAAAATGGAATTTGAACCATTTCAGTCTCTTGTCCCTAA10240.10960956942920604No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC10190.10907436645347748No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT10080.1078969199068747No Hit
GTTTAAGGTCTCCAACATCTTTGCAGTCATCAAAGTCTACTTTTTCTGGT10070.10778987931172898No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG9950.10650539216998046No Hit
GTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTTGA9940.10639835157483475No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT9700.10382937729133775No Hit
ATGTAGATGGATTCGAGCCGAACGGCTGCATTGAGGGCAAGCTTTCCCAA9640.10318713372046348No Hit
GTATTCAGTAGCCCTGCAGTGGGACACTTCTGCTGTAAAATAGTTCCTCC9520.10190264657871499No Hit
GCGGAAAAGGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAA9400.10061815943696648No Hit
CATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTT9360.10018999705638365No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCCG100.0070889336145.01268145
AGCGAAA550.0118.697571
GCAGGTA2400.0114.801712
AGTAGAA3500.0109.8423541
CAGGTAC2550.0108.03713
GGTACTG2700.096.664775
GCGAAAG700.093.2224352
AATTATA259.383292E-487.007614145
GGTACTA259.3872834E-486.99835
CGAAAGC850.085.292453
TCCACCT300.001931745272.537411
GATCTTA502.2990644E-672.537411
CCTACTA300.001935852572.498585
AGGGTGA502.3063894E-672.498586
GTACTAA300.001935852572.498586
GCAGGGT502.3063894E-672.498584
GTAGAAA5350.071.828712
AGGTACT3950.067.9100654
GACAGTA3600.066.457037
CAGGGTG554.061867E-665.90785
Produced by FastQC (version 0.11.5)