FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S4_R2_H11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S4_R2_H11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences623059
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC22850.3667389444659334No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT20930.33592324322415695No Hit
GTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAG12820.2057590051664449No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC12500.20062305495948218No Hit
CCCTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGG12140.19484511097664908No Hit
GGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTATGG11220.1800792541316312No Hit
GTACAGTGGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTC10740.17237532882118708No Hit
TAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAGGC10410.16707888017025674No Hit
CCATTACACAAATCATTTGGCTGGGAATGGAACATTTACCATATGTATGA9920.15921445641584503No Hit
CTTTTAGACTAGGAAGGAAGGGAAGTACAGTGGTTATGTATTTGGATGTA9250.1484610606700168No Hit
ACCTAAGACATAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATAT8040.12904074894993894No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA7970.12791725984216581No Hit
GATTAGCACACAGTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAAC7720.12390479874297619No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG7710.1237443002990086No Hit
GTGTACACCCAGCCTTTGAACATTTTTATCTGTGGCTGCATTCATGCCAG7460.11973183919981895No Hit
GTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTATGGGAGCTT7440.11941084231188379No Hit
CACACAGGAGCTATCAATCACTCTCGTCTCCCTGGTTTCTGTATGCACTC7320.11748486098427276No Hit
AGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAGAACT7240.11620087343253208No Hit
TTTCAAACATGAATCCGTAGACCATTTCCTCCACCTAAGACATAATAACA6840.10978093567382864No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG6760.10849694812208796No Hit
TACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGGGTTTCA6750.10833644967812037No Hit
GTGTATTCCTTCCCCATCTTGGGAATTCTAGTTAGTCGAAGAGCAATACT6580.10560797613067142No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA1250.063.8269921
AGCTATC2450.062.1392149
GTAGAAA5850.050.8334581
CGAAAGC1600.049.8448223
GTATAGA803.723709E-545.331671
GGTCGTT1505.366019E-1043.500947
TTATACT4100.040.671614
GCGAAAG2005.456968E-1239.8790632
GTATACC1859.640644E-1139.1900334
AGGTCGT1803.1959644E-936.2507826
TATACCC2252.0008883E-1135.445215
CTTGCGT1707.791277E-834.12934145
TTAAGAT6800.034.118384
TAAGATG7100.033.697915
GGATAGC1751.0079566E-733.1435745
CCTTATA4200.032.800962
GCATTAA7650.032.235851
TAGTATG900.0036253832.2255062
GCAGGTA2705.456968E-1232.2255022
CCCTTAT4300.032.0484351
Produced by FastQC (version 0.11.5)