FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S4_R2_H10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S4_R2_H10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences837201
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA20320.24271351802016483No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT20250.24187739861753632No Hit
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC19890.23757735597544674No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG17100.20425202549925286No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT14660.17510729203620157No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC13010.15539876325995788No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA12240.14620144983104416No Hit
GTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAG11740.1402291683836976No Hit
CCCTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGG11030.13174852872846543No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC10890.1300762899232084No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC10860.1297179530363676No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA10460.12494012787849036No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT10080.12040119397850695No Hit
GGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTATGG9810.1171761619969398No Hit
GTACAGTGGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTC9480.11323445624169105No Hit
TAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAGGC8760.10463437095751199No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG8640.1032010234101488No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG8600.10272324089436108No Hit
CCATTACACAAATCATTTGGCTGGGAATGGAACATTTACCATATGTATGA8390.10021488268647553No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2300.059.911521
AGTAGAA9550.057.7158051
CGAAAGC2450.056.2199483
CAATTCG5700.053.416749
GTAGAAA10650.051.732912
GCGAAAG2850.048.329432
AGCTATC2850.045.7857789
ATTAAGA7250.044.996373
TAGAAAC13250.044.3171773
TTAAGAT7400.043.1046334
ACTGATC5850.042.1333548
TAAGATG7900.041.2941365
TGGTGCG1953.6379788E-1240.8941358
CAACCGT907.484957E-540.274536
GATTAGC4600.036.2622341
GTCTAGT5450.033.2681051
CGGTTCG1751.00588295E-733.154041
GGTTCGC1551.4655543E-632.7392922
GACAGTA4950.032.219627
ACAATTC9550.031.8822428
Produced by FastQC (version 0.11.5)