FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S4_R2_H07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S4_R2_H07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences738922
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC13280.17972126963333074No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA9350.12653568306262367No Hit
GACCTGGAGTGACCAGGACCAGCAGCTGGCGAGTCACTGCTGATGGCAAA9150.12382903743561567No Hit
GCTCTAGGAAGTGGCCCGAAGGAGGTCAGGAGTGTCCTTCATTCCCTCCC8000.1082658250803197No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGCG256.4662527E-6115.990978
ACCGCGA301.5998006E-596.6591349
AGCGAAA3100.067.844841
TTTCGCG350.003562457262.159046145
CGAAAGC3700.056.8237423
GCGAAAG3750.056.0698852
ATACCGC651.0938682E-555.7648857
TATACCG657.335704E-444.6149256
AGTAGAA7400.044.102311
GTAGAAA8400.043.1689261
GCGCCTT1651.3606041E-939.555756145
GATTAGC2700.034.9188651
TAGAAAC10250.033.9531672
GGATAGC2603.6379788E-1233.4611975
TACGGTT1102.4356841E-432.954214
GTATTGG3600.030.2182461
CGCGACA200.006078862628.99773810-14
GATAGCA3153.45608E-1127.6187636
GACAGTA4200.027.6168967
ACCGTAC1100.009654527526.3722881
Produced by FastQC (version 0.11.5)