FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S4_R2_H03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S4_R2_H03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1224971
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3800.040.0834271
GTATATA10050.037.528951
GTATTAC8100.027.7591291
CGAAAGC5900.027.0369643
TATAAGG5000.026.1029622
TATACGC1550.001807526723.3897512
GCGAAAG6900.022.067722
GTCGTGG1650.00259701321.975779145
TAACGTA3055.336351E-721.395874
ATATATG8650.020.9561353
GGTATGA9100.020.7132538
TATATAT13050.020.002272
TATATGT7650.019.9042194
TACTATA4402.2610038E-919.7749712
TATACTG5505.456968E-1219.7749715
AAGGTAT8850.019.6632486
TTACATC10100.019.3833874
GTACTAT3751.8006358E-719.3418451
TATATAC11000.019.1158053
AACGTAG3151.6823868E-518.4147875
Produced by FastQC (version 0.11.5)