FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S4_R2_H01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S4_R2_H01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences765444
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2450.047.360961
TTGCGCA1401.4084435E-841.446255145
TATAAGG3052.3646862E-1128.5274432
TTACGCA1804.6968016E-628.1954254
CTATAAG3453.6379788E-1227.3269311
TATATAG2456.4321284E-826.633585
GTATATA9650.025.5516031
GTTACGC2001.064756E-525.375883
TATACAG5150.025.3406855
GTATTAG3151.029548E-925.3249571
GTATTAT4600.025.224861
TAATACT3301.7698767E-924.1675054
CAATTCG2101.5556048E-524.1643499
ATATATG4251.8189894E-1223.883183
CTAATAC2151.8644949E-523.6054693
GCGAAAG5050.022.9725952
CGAAAGC5050.022.9710923
TATATAT5400.022.8263572
CAGCTCG5750.022.6934749
TAGTGCA2003.1013894E-421.7507554
Produced by FastQC (version 0.11.5)