FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S4_R2_G06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S4_R2_G06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences885753
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG8940.10093107220636001No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATTCG3550.051.053049
AGCGAAA2300.050.451461
CTAGTAC1157.1449504E-637.8236433
CGAAAGC3400.036.2476583
GTGATCG1406.6118264E-736.2476589
TATACAG4400.036.2476585
AGTAGAA5800.035.011571
GTTGACG900.003629157632.220149
GCCTAAT1402.740128E-531.0817011
GCGAAAG4150.029.6968782
TATATAT5750.027.7373372
TATATAC8000.026.2795493
GTATTAC4200.025.9014171
TATATGT6600.025.263524
TAGAAAC8350.025.1780153
TTTAAAC8150.024.9063683
GTAGAAA8250.024.6044712
ATAGACC3251.4861143E-924.5368773
GTATATA8050.024.324811
TATACTG8050.024.3151975
Produced by FastQC (version 0.11.5)