FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S4_R2_G04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S4_R2_G04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences981054
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT16360.16675942404801367No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2600.089.248971
CGAAAGC4100.056.5853653
TATAGAC8950.040.5027962
ATTACGC907.482339E-540.277783
ATAGACC7900.038.5443043
TTCAACG1701.8299033E-938.3803987
GATTACG951.0288766E-438.1578942
GTATTAC5950.034.1246071
TATTACA6950.033.3812942
CTAATAC3650.031.7808233
TATACAG3500.031.0714265
TCAACGT2303.4893674E-828.3681188
GCGAAAG8450.028.313612
ACAGTCG1555.5022283E-528.0630868
ACCTCTT11050.026.899087
AGGACCG3255.0931703E-1126.769235
TAGGTAC12800.026.629236145
ATTACAT8300.026.2048193
TAGACCT11300.025.6637174
GGACCGT4550.023.90116
Produced by FastQC (version 0.11.5)