FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S4_R2_G01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S4_R2_G01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1421798
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG19100.13433694519193304No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA4000.070.70021
CGAAAGC5450.053.2074743
GCGAAAG6100.047.5378232
CAATTCG6550.034.3106969
TATATAC9600.029.4511683
TAGTACG2354.3030013E-827.7641124
TATATTC9750.026.7674523
GTATTAC5550.026.1308231
TAGAAAC14650.025.7320792
ACTGATC10450.025.668158
TAGACAG9100.025.4928115
TATACAT6650.023.983375
TCGTATA3753.092282E-1023.1984582
AGTAGAA12550.023.1117231
ATATTCA10200.022.7435874
GGTCGTT6100.022.5804657
GTTACGC4505.456968E-1222.5540563
GTATATA9650.022.5429121
ATAGACC5150.022.5227743
TATACGT1952.6166718E-422.306214
Produced by FastQC (version 0.11.5)