FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S4_R2_F12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S4_R2_F12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1327136
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTTAGGCAGAACCCAACAGAAGAGCAAGCCGTGGATATATGCAAGGCT44480.3351578135172281No Hit
GGATGTAATCATGGAAGTTGTTTTCCCTAACGAAGTGGGAGCCAGGATAC40300.30366141827212884No Hit
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG40030.30162696211993345No Hit
GCCTAAGGATGTCTACCATCCTAATTCCACCAATCTGTGTGCTGTGGCAC38970.29363983796687No Hit
GATATATGCAAGGCTGCAATGGGACTGAGAATTAGCTCATCCTTCAGTTT36100.2720143225713114No Hit
GTATAGCATTGTAGGGTTCTGGGACCACTGAATTTTAACAGTTTCCCAGT34900.26297229522822074No Hit
CTTCATATCCCTCATGCACTCTTATCTTCAATGTTTGAAGATTGCCCGTA32530.24511429122561668No Hit
GTGCTGGAGTACTCATCTACACCCATTTTGCTGATTCTCACTCCTCTCAT30140.2271055867672944No Hit
CCACTACCCTCTCCGTGCTGGAGTACTCATCTACACCCATTTTGCTGATT30100.22680418585585804No Hit
GATGTATACTCCAGGAGGGGAAGTGAGGAATGATGATGTTGATCAAAGCT29710.22386552696935358No Hit
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA29540.22258457309574906No Hit
ACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT29010.21859101101921732No Hit
GTAGTACATTTCCTCGTTGGTCCCGGATTCTCAAAAAACGGTCAATGCTC27000.20344561521954044No Hit
GCATTGTAGGGTTCTGGGACCACTGAATTTTAACAGTTTCCCAGTTTCTG25120.1892797723820317No Hit
GATATGAAGAGTTCACAATGGTTGGGAGAAGAGCAACAGCCATACTCAGA24950.18799881850842717No Hit
ATCCCAATCATTCCCATCACATTGTCGATAGGTTCAACTCCCCAATTTTG23710.17865539025390012No Hit
ATCTTATCATGTCTGGATCCGCTGCATTAATGAATCAGGGGATAACGCAG23570.17760048706387288No Hit
GCATATATCCACGGCTTGCTCTTCTGTTGGGTTCTGCCTAAGGATGTCTA23450.1766962843295638No Hit
CTCATTGACATCTCGATGCTTGGAGTCATGTCTGGCAATATCCCAATCAT23090.17398367612663662No Hit
GTCGATAGGTTCAACTCCCCAATTTTGAAAAAGCACTTTCGCATCCTTCT22830.1720245702023003No Hit
TTCATATACTGATGACCTCTTTATAGCCAACCTTTGTTCATGGCAGCCAG22070.1662979528850095No Hit
CTCCAGGAGGGGAAGTGAGGAATGATGATGTTGATCAAAGCTTGATTATT21930.16524304969498227No Hit
GTGTGTACATTGAAGTGTTGCATTTGACTCAAGGAACATGCTGGGAACAG21850.16464024787210957No Hit
GTATTGACCAACACTGATTCAGGACCATTAATCTCCCACATCATTGACGA21240.16004388397270514No Hit
CCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTT21050.15861222964338245No Hit
ATGTACTACTGTCTCCCGAGGAGGTCAGTGAAACACAGGGAACAGAGAAA21050.15861222964338245No Hit
ATTCTAGACGGGCCGGCCCCTGCGTGTGGCACGGGCGGCCGGGAGGGCGT21010.1583108287319461No Hit
GTATTGGCCTCTAATGGCCTTAGGTACTAAAGACTGAAATGGTTCAAATT21010.1583108287319461No Hit
GCATTAATGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGC20950.15785872736479156No Hit
GAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGG20570.15499541870614617No Hit
CAATTATTGCTTCGGCAATCGACTGTTCGTCTCTCCCACTCACTATCAGC20330.15318701323752804No Hit
GGCTAAAGCATGGAACCTTTGGCCCTGTCCATTTTAGAAACCAAGTCAAA20310.15303631278180985No Hit
GAAGATAAGAGTGCATGAGGGATATGAAGAGTTCACAATGGTTGGGAGAA19970.15047440503460083No Hit
CTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCT19820.1493441516167145No Hit
TCTCAGTGCCAAGGAGGCACAGGATGTAATCATGGAAGTTGTTTTCCCTA19710.1485152991102645No Hit
CCCATATGTCCTTCCGAGTGAGAGACACAAAAAATTCCAACACACTATTG19550.14730969546451908No Hit
GTGTACATTGAAGTGTTGCATTTGACTCAAGGAACATGCTGGGAACAGAT19550.14730969546451908No Hit
CTCTTATCTTCAATGTTTGAAGATTGCCCGTAAGCACCTCTTCCTCTCTC19210.14474778771731006No Hit
GATAAAAGCAGTCAGAGGTGATCTGAATTTCGTCAATAGGGCGAATCAGC18910.1424872808815374No Hit
AACAAATACAGTTCATTATCCAAAAATCTACAAAACTTATTTTGAAAGAG18770.14143237769151012No Hit
CTTATCTTCAATGTTTGAAGATTGCCCGTAAGCACCTCTTCCTCTCTCTT18630.14037747450148289No Hit
TCCTCATTCTGGGCAAAGAAGACAAGAGATATGGGCCAGCACTAAGCATC18270.1376648662985557No Hit
CCTTTGGCCCTGTCCATTTTAGAAACCAAGTCAAAATACGTCGGAGAGTT18110.13645926265281025No Hit
TTCTCACTCCTCTCATTGACATCTCGATGCTTGGAGTCATGTCTGGCAAT18110.13645926265281025No Hit
TCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAGGCTAATGTGCTAATT18100.13638391242495115No Hit
GTCCATTTTAGAAACCAAGTCAAAATACGTCGGAGAGTTGACATAAATCC18070.1361578617413739No Hit
CCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGG18060.1360825115135148No Hit
GTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAG17770.13389735490560123No Hit
ATGCTATACAATAAAATGGAATTTGAACCATTTCAGTCTTTAGTACCTAA17590.13254105080413764No Hit
CTATCAGCTGAATCAATCTCCTGGTTGCTTTTCTGAGTATGGCTGTTGCT17530.1320889494369831No Hit
GTATACATCTGTTCCCAGCATGTTCCTTGAGTCAAATGCAACACTTCAAT17460.13156149784196947No Hit
GTTCTGGGACCACTGAATTTTAACAGTTTCCCAGTTTCTGATGATCCATT17370.13088334579123767No Hit
AGATAAGAGTGCATGAGGGATATGAAGAGTTCACAATGGTTGGGAGAAGA17020.12824608781616956No Hit
TTATTGTATAGCATTGTAGGGTTCTGGGACCACTGAATTTTAACAGTTTC17020.12824608781616956No Hit
CATCTACACCCATTTTGCTGATTCTCACTCCTCTCATTGACATCTCGATG16960.12779398644901505No Hit
CTAATGGCCTTAGGTACTAAAGACTGAAATGGTTCAAATTCCATTTTATT16830.12681443348684684No Hit
GGATACTAACATCGGAATCGCAACTAACGATAACCAAAGAGAAGAAAGAA16680.1256841800689605No Hit
GCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCT16360.12327297277746968No Hit
GAATTAGCTCATCCTTCAGTTTTGGTGGATTCACATTTAAGAGAACAAGC16320.12297157186603332No Hit
CTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGT16250.12244412027101971No Hit
CTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGG16210.12214271935958333No Hit
ATGCAAGGCTGCAATGGGACTGAGAATTAGCTCATCCTTCAGTTTTGGTG16140.12161526776456973No Hit
GTATTTTCACAAGAGGATTGTATGATAAAAGCAGTCAGAGGTGATCTGAA16110.12138921708099246No Hit
TTTCAGAAGGATGCGAAAGTGCTTTTTCAAAATTGGGGAGTTGAACCTAT15940.12010826320738793No Hit
CTCTTGACTGATGATCCGCTTGTTCTCTTAAATGTGAATCCACCAAAACT15910.11988221252381068No Hit
GTCCTGGGGTTGACCAGAGGGCCCCGGGCGCTCCGTGTGTGGCTGCGATG15810.11912871024521979No Hit
TTATTGGAGATGTGCCACAGCACACAGATTGGTGGAATTAGGATGGTAGA15550.11716960432088346No Hit
GGGTAGTGGTGAGCATTGACCGTTTTTTGAGAATCCGGGACCAACGAGGA15550.11716960432088346No Hit
GTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGC15410.11611470113085622No Hit
GTATCAAATGTCCCAAGCACATCCCTCATTTGTTGGAACAGAGTTCTTAC15400.11603935090299713No Hit
GCTTTTATCATACAATCCTCTTGTGAAAATACCATGGCCACAATTATTGC15330.1155118993079835No Hit
CAATAATTGTGGCCATGGTATTTTCACAAGAGGATTGTATGATAAAAGCA15310.11536119885226531No Hit
GAATGAGGAATCCCCTCAGAACAGCGGACTCCACTCCAGCTGTGCCTTCA15270.11505979794082896No Hit
CTACAATGCTATACAATAAAATGGAATTTGAACCATTTCAGTCTTTAGTA15120.11392954452294266No Hit
TCTGTGTGCTGTGGCACATCTCCAATAAAGATGCTAGTGGATCTGCTGAT15000.11302534178863356No Hit
GTATACTCCAGGAGGGGAAGTGAGGAATGATGATGTTGATCAAAGCTTGA14940.11257324042147902No Hit
TCCTAATTCCACCAATCTGTGTGCTGTGGCACATCTCCAATAAAGATGCT14810.11159368745931088No Hit
GGATATGAAGAGTTCACAATGGTTGGGAGAAGAGCAACAGCCATACTCAG14770.11129228654787453No Hit
GGCCAATACAGTGGGTTTGTAAGAACTCTGTTCCAACAAATGAGGGATGT14750.11114158609215634No Hit
CTTCTGAAAATGTCTTAAAAGTTGATGCATAGGATTCAATCGCTGATTCG14610.11008668290212908No Hit
GTATTTGTTATTGGTCCATTCCTATTCCACCATGTCACAGCCAGAGGTGA14440.10880572902852459No Hit
TCTCTGTTCCCTGTGTTTCACTGACCTCCTCGGGAGACAGTAGTACATTT14440.10880572902852459No Hit
TGTTATAGTTGAATACAGGAGAATTGCCCCTTACAAGTATTCTCATTCCT14400.10850432811708823No Hit
GTACTACTGTCTCCCGAGGAGGTCAGTGAAACACAGGGAACAGAGAAACT14380.10835362766137005No Hit
ATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGC14370.10827827743351096No Hit
GTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCAT14360.10820292720565187No Hit
TAATAAAACTTCTTCCCTTCGCAGCCGCTCCACCAAAGCAAAGTAGAATG14350.10812757697779278No Hit
TGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATC14350.10812757697779278No Hit
GTATCAGCAGATCCACTAGCATCTTTATTGGAGATGTGCCACAGCACACA14310.10782617606635643No Hit
CTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCC14270.10752477515492008No Hit
CTCATGCACTCTTATCTTCAATGTTTGAAGATTGCCCGTAAGCACCTCTT14260.10744942492706099No Hit
GGATTCAATCGCTGATTCGCCCTATTGACGAAATTCAGATCACCTCTGAC14210.10707267378776554No Hit
CCTCTGACTGCTTTTATCATACAATCCTCTTGTGAAAATACCATGGCCAC14080.10609312082559738No Hit
GTGCATGAGGGATATGAAGAGTTCACAATGGTTGGGAGAAGAGCAACAGC14050.10586707014202011No Hit
CTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGT14030.10571636968630194No Hit
CTCCAACATGTATGCAACCATCAAAGGAGAAATTTTGCAATCCTGGAGTT14010.10556566923058375No Hit
GTCCAAACTCATCAATGTATCTTATCATGTCTGGATCCGCTGCATTAATG13950.10511356786342921No Hit
GAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTT13940.10503821763557014No Hit
GGTCAATACCTATCAATGGATCATCAGAAACTGGGAAACTGTTAAAATTC13770.10375726376196562No Hit
TCCATTGATAGGTATTGACCAACACTGATTCAGGACCATTAATCTCCCAC13770.10375726376196562No Hit
CAGTAGTACATTTCCTCGTTGGTCCCGGATTCTCAAAAAACGGTCAATGC13750.10360656330624744No Hit
CTTTATAGCCAACCTTTGTTCATGGCAGCCAGCATATGGGCATATGTTGC13720.10338051262267017No Hit
GTGAAAATACCATGGCCACAATTATTGCTTCGGCAATCGACTGTTCGTCT13700.103229812166952No Hit
CATAAATCCTGGTCATGCAGATCTCAGTGCCAAGGAGGCACAGGATGTAA13690.1031544619390929No Hit
GCATGGAACCTTTGGCCCTGTCCATTTTAGAAACCAAGTCAAAATACGTC13640.10277771079979746No Hit
GTTCTGAGGGGATTCCTCATTCTGGGCAAAGAAGACAAGAGATATGGGCC13640.10277771079979746No Hit
CTCCTGGAGTATACATCTGTTCCCAGCATGTTCCTTGAGTCAAATGCAAC13620.10262701034407927No Hit
TCTTCAAACATTGAAGATAAGAGTGCATGAGGGATATGAAGAGTTCACAA13480.10157210715405203No Hit
ATTGAATCCTATGCATCAACTTTTAAGACATTTTCAGAAGGATGCGAAAG13360.10066790441974297No Hit
GTTCATTGATGCTTAGTGCTGGCCCATATCTCTTGTCTTCTTTGCCCAGA13350.10059255419188387No Hit
GTCAAGAGAGAGGAAGAGGTGCTTACGGGCAATCTTCAAACATTGAAGAT13310.10029115328044752No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACCGT100.0070829117145.0562145
TTACGGC100.0070829117145.0562145
TTTCGCG501.8189894E-12116.04496145
GTCGGCC203.8603836E-4108.792145145
CGCTGGG301.5965776E-596.70414145
CGATTAC259.372989E-487.033714145
GCTTGCG300.001932908272.52811145
ATCTCAC406.651209E-572.5281145
GCCCTGG406.651209E-572.5281145
TATATAT7850.071.120772
TACCGGC754.84215E-967.69289145
GTATATA8200.067.2084961
AGATGGG554.060261E-665.914762
TTAGGGG1450.065.0006944
TAGGGGC1450.064.998245
AGGAATA702.3288703E-762.166943145
CATAAAA350.00356128962.166943145
CTAATTA1650.061.538998145
GAGATGG606.7987858E-660.4286961
ATATGAG9400.060.1579485
Produced by FastQC (version 0.11.5)