FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S4_R2_F05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S4_R2_F05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences613667
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT12440.2027158051516539No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACATCTTAACAATA10530.17159143313881958No Hit
GTTTATATGAGAAAGGAGGGGAATGAAAGAGAAAATAGAATCAAAACACC7920.1290602232155224No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA1850.070.55581
TATAACG604.9442635E-448.3319744
TACGGGT657.335374E-444.614134
GCGAAAG3200.040.783432
CGAAAGC3400.038.3812753
ATAACGT900.00362764632.2213175
TATACAG3850.032.0120855
TATAGAC7350.030.5798652
GTACTAG1306.4718793E-427.890651
ATAGACA8600.026.9759863
TAGACAT8700.026.6659184
TATACGA1100.00966621526.3650442
GTATAGG2507.80401E-826.105651
GTATATA5100.025.5937711
GTATTAC6350.025.1235451
ATTACAT6450.024.7279873
ATACAGC4950.023.4336856
GCTTATA1550.001805422223.3921571
TATAGTA1550.001807135223.3883442
TATTACA8100.023.2728462
Produced by FastQC (version 0.11.5)