FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S4_R2_F02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S4_R2_F02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences895279
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT27370.3057147548417868No Hit
GTGTATACTCCTACAGACACATTAGTCTTTGGAGGCAATTTTTTGCATAG26270.29342808219560607No Hit
CTATGTGTACTGCATAACAAACCGCTCCCACCTAACTAAGGAATTTCAAA16080.17960881468235043No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACCTCTTAACAATA15620.17447075157576575No Hit
GTATACTCCTACAGACACATTAGTCTTTGGAGGCAATTTTTTGCATAGCT11560.1291217598089534No Hit
GCATAACAAACCGCTCCCACCTAACTAAGGAATTTCAAAAAGAGTCCCTC10870.12141466514907642No Hit
GTCTTGGCACTGCTCGGCCGCCTCCATCCATGTCTGCTGCTGGCCTTGCT10730.11985090681228978No Hit
ACACTGGGGATCCCACACTTGGCCGTTATGGGAGGCCCGGCTAGAGTCAG10700.11951581574012123No Hit
GCTTCAATATCCCCATGCAGTTAAAAATATACAACATTGAAGATCGAACA10220.11415435858542422No Hit
GTATGGATTTGGAGTTAAATGGGTTGGAATCTGGAAATGGGGATGAAGAA9660.10789932523827767No Hit
GTGTTGGAGCGCTATGTGTACTGCATAACAAACCGCTCCCACCTAACTAA9640.1076759311901653No Hit
CCTACAGACACATTAGTCTTTGGAGGCAATTTTTTGCATAGCTTCAATAT9610.10734084011799674No Hit
CTTCTTGTGCAGTGGCAGAGACTCCAACTTATCTACAAGACAGCGGAGGC9390.10488350558876061No Hit
TTCTAGCACTGGTGAGGCTTGCAGGTGCCAAGGCAGGAGGGGTTCTTGAG9280.10365483832414253No Hit
ATCGGGACTCCAGTGAGGGCTAACTTGGGATCGCTGTTGGCAAGCTCCTC9220.10298465617980541No Hit
GTTTATATGAGAAAGGAGGGGAATGAAAGAGAAAATAGAATCAAAACACC9110.10175598891518733No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2750.094.9464651
GATAATA406.649821E-572.528551
CTAATTA300.001933940172.516396145
GCGATCA1000.072.496159
GCGAAAG3750.069.604082
GATATAG350.003560822762.1673281
TAACCTA502.0101032E-458.0001566
TAGACTA400.006050615654.375155
GGGACCG400.006050615654.375155
AGTTCGT400.006051952554.3721128
AGGCGAT1353.6379788E-1253.703857
GGCTATA1650.052.7303622
CGAAAGC5400.048.3334663
GTATACT8300.048.0422973
GTATTAC5000.047.8688471
TGTATAC8350.047.7572862
GGCGATC1652.910383E-1143.937068
GGGCTAT2000.043.517131
TATAGAC8300.042.8037072
GAATAAG1201.967037E-742.3083231
Produced by FastQC (version 0.11.5)