FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S4_R2_E10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S4_R2_E10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences475028
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT15280.3216652492063626No Hit
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC11620.2446171594095506No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC10760.22651296344636523No Hit
GTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAG7370.15514874912636728No Hit
CCCTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGG7240.1524120683412346No Hit
TAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAGGC6650.1399917478548633No Hit
GGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTATGG6460.13599198363043863No Hit
ACCTAAGACATAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATAT6040.12715039955539464No Hit
GTACAGTGGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTC5980.12588731611610263No Hit
CACACAGGAGCTATCAATCACTCTCGTCTCCCTGGTTTCTGTATGCACTC5620.11830881548035063No Hit
CCATTACACAAATCATTTGGCTGGGAATGGAACATTTACCATATGTATGA5200.10946723140530665No Hit
GTGTACACCCAGCCTTTGAACATTTTTATCTGTGGCTGCATTCATGCCAG5090.1071515784332713No Hit
CTTTTAGACTAGGAAGGAAGGGAAGTACAGTGGTTATGTATTTGGATGTA5030.1058884949939793No Hit
GATTAGCACACAGTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAAC4880.10273078639574931No Hit
TTTCAAACATGAATCCGTAGACCATTTCCTCCACCTAAGACATAATAACA4830.10167821686300597No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC1400.056.9581223
TCGTTAC400.006043970654.386291
AGGCGAT450.00964016348.3281066
GCATTAA5700.041.9824031
AGCGAAA1903.6379788E-1241.9824031
GTACTAA1401.4091711E-841.4371721
ATTAAGA6150.038.898233
CGTTACA750.001482622738.6665532
CATTAAG5650.038.4954642
TAAGATG5650.038.491415
CTATGAC1354.9682603E-737.5885283
TTAAGAT6000.037.454284
AGCTATC2451.8189894E-1235.5063639
GTCTATA1251.2618955E-534.8072281
TTAGTAC3750.032.8631133
TCAATAC900.003628249532.218743
AGATGTA7200.032.218747
GCGAAAG2506.91216E-1131.8999062
GTGTACG2506.91216E-1131.8965479
GTCTAGT3450.031.5282881
Produced by FastQC (version 0.11.5)