FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S4_R2_E06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S4_R2_E06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences627435
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGAGCATGGCCAGCCACCCATTGAGCGCAAGGTTGTGGAGCTGCCC20220.32226445767290635No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG16830.2682349566090511No Hit
CCCCCATTGCTGTGCCAGACGCAACTCTACTCGCCCAACCTGCAGGGCCG14080.22440571533306242No Hit
TTCCCAGGCTGCATCAATAATGCTGACCTCTTTGAAGACCAGGTAAACTG12330.19651437997561502No Hit
CAATAATGCTGACCTCTTTGAAGACCAGGTAAACTGGCACCTCAGGAAGG10700.17053559332839258No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT9340.14886004127917632No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA9110.14519432291791182No Hit
GTTCACGGCCTGTGGCTGGGCACTAGGCCAAGTGCCATCCTTGTTTCGGG8360.13324089347900578No Hit
CTTGAGGCCCACCCCCATTGCTGTGCCAGACGCAACTCTACTCGCCCAAC8190.13053144947285375No Hit
CCTCCAATGCAGTGTTCACGGCCTGTGGCTGGGCACTAGGCCAAGTGCCA7710.1228812546319539No Hit
GTCTTGAGGGCAGCGTCGAGCACTGTGACTCGTGTGTTACACAATCTCTC7500.11953429438906023No Hit
GACAAGGACTCTGGCACCTTCCCAGGCTGCATCAATAATGCTGACCTCTT7420.11825926191557691No Hit
GTTGTGGAGCTGCCCAGCGTCCAAAAGGTCGAAGTGTATCCAGTAGAGCT7170.11427478543594156No Hit
ACCCAATCCTCCTCACTTGAGGCCCACCCCCATTGCTGTGCCAGACGCAA7100.11315913202164368No Hit
CCCTTAGGCAGGGGTGTGTTCCTAAAGTCTGGATGTGCACTGGCATGCAT7010.11172472048897496No Hit
GCCTAAGGGAGGGCCACCTGCTCAGCCTCAAACCGAGCCCTAGTCTACCG6820.10869651836445209No Hit
GTATATATGTATGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAA6810.10853713930526668No Hit
CAACCCACACTGCTCAATTCAGCCGTACGGATTCTGTTGACCTCGTTCTG6710.10694334871341254No Hit
CTTTGAAGACCAGGTAAACTGGCACCTCAGGAAGGGTCTAGTGGAAGGTG6630.10566831623992923No Hit
CCTCAGGAAGGGTCTAGTGGAAGGTGAGGATTATGTGCTGCTCCCAGCGG6530.1040745256480751No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGTA100.0070923353144.985649
AGCGAAA2050.0109.701981
GATACGG259.3620695E-487.053831
GCGAAAG2650.084.809692
CGAAAGC2800.080.266313
ATAAGCC300.001935552872.4986043
CGTTACA350.00356615662.1416632
GTATAGG1550.060.8440741
ATAAGGT400.006050300354.3739552
CGGACTA400.00605220854.3696175
TAGGGAG907.48361E-540.2737965
GTTAGGC1157.108214E-637.849491
TATACAG6350.037.673445
TATAGAC2201.6370905E-1136.2493022
TATATAG1209.564659E-636.2464145
ATATATG4200.034.5231443
GATCCAT850.002742540334.1142736
ATAGACC2404.3655746E-1133.2285273
CTTATAA1102.4262916E-432.9749371
CTCCATA1551.4567977E-632.762191
Produced by FastQC (version 0.11.5)