FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD-S4_R2_D05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD-S4_R2_D05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1331342
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG14160.10635884693790175No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA4100.047.7598721
CGAAAGC4600.045.7080733
GTCGCAT800.002033515636.2621271
TATACAG9350.031.7925225
GCGAAAG6600.029.6600992
TATTACG1050.007708077327.6199862
TATATAC17950.027.4661123
GTATATA18250.024.2409821
GCTTTCG1801.5172083E-424.174751
CAGCTCG11200.023.9490059
ATATACT10000.023.9258124
GTATAAG5200.022.3151531
GATACCG2303.1422085E-522.0659665
TATATGT11200.022.0096744
TACCGAT2003.1046502E-421.7491027
CTCGCGC3055.3257463E-721.4006145
ATATAAC4109.385985E-1021.2202323
TATAGAC9300.021.04912
TATACCG2104.314179E-420.7149895
GTACGGC1800.004299476720.1456241
Produced by FastQC (version 0.11.5)